High-performance glucose-sensitive nanocapsules

• Project/Area Number: 19390010
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: ANZAI Jun-ichi Tohoku University, 大学院・薬学研究科, 教授 (40159520)
• Co-Investigator(Kenkyū-buntansha): SATO Katsuhiko 東北大学, 大学院・薬学研究科, 助教 (80400266) ; 星 友典 東北大学, 大学院・薬学研究科, 講師 (50302170) ; 江川 祐哉 東北大学, 大学院・薬学研究科, 助教 (90400267)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2007: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: グルコース / ナノカプセル / インスリン / ミクロカプセル / 糖応答性 / 高分子電解質 / 累積膜 / グルコースセンサー / フェニルボロン酸 / フエニルボロン酸 / グルコース応答性 / 交互累積法 / センサー

Research Abstract

The development of nanocapsules for glucose determination and for release insulin in response to enhanced glucose level have been studied. CaCO_3 nanoparticles containing lectin complexes or insulin were coated with polyelectrolyte thin films followed by dissolution of the nanoparticle, resulting in nanocapsules. The nanocapsules thus prepared were successfully used for glucose determination and for insulin release in response to enhanced glucose level.

Research Products

• [Journal Article] Sugar-dependent solubility and fluorescence property of copolymers consisting of phenylboronic acid and 2-hydroxyethyl methacrylate moieties (2010)
  • Author(s): Katsuhiko Sato, Tatsuya Nakajima, Yu Yasukawa, Jun-ichi Anzai
  • Journal Title: Polymer Bulletin (印刷中)
  • Peer Reviewed
• [Journal Article] Layer-by-layer polyelectrolyte films containing insulin for pH-triggered release (2010)
  • Author(s): Kentaro Yoshida, Katsuhiko Sato, Jun-ichi Anzai
  • Journal Title: Journal of Materials Chemistry 20 Pages: 1546-1552
  • Peer Reviewed
• [Journal Article] Layer-by-layer polyelectrolyte films containing insulin for pH-triggered release (2010)
  • Author(s): Yoshida, Kentaro
  • Journal Title: Journal of Materials Chemistry 20 Pages: 1546-1552
  • Peer Reviewed
• [Journal Article] Layer-by-layer thin films containing proteins for biosensor applications (2009)
  • Author(s): Katsuhiko Sato, Jun-ichi Anzai, Jiadong Huang, Qiang Chen
  • Journal Title: Bottom-up Nanofabrication 5 Pages: 219-233
• [Journal Article] Preparation of dendrimer-loaded microcapsules by a layer-by-layer deposition of polyelectrolytes (2009)
  • Author(s): Shigeru Tomita, Katsuhiko Sato, Jun-ichi Anzai
  • Journal Title: Materials Science and Engineering C 29 Pages: 2024-2028
  • Peer Reviewed
• [Journal Article] Sugar response of boronic acid-substituted azobenzene dye-modified polymer (2009)
  • Author(s): Yuya Egawa, Ryota Gotoh, Toshinobu Seki, Jun-ichi Anzai
  • Journal Title: Materials Science and Engineering C 29 Pages: 115-118
  • Peer Reviewed
• [Journal Article] Preparation of insulin-containing microcapsules by a layer-by-layer deposition of concanavalin A and glucose (2009)
  • Author(s): Katsuhiko Sato, Daisuke Kodama, Yoshihiro Endo, Jun-ichi Anzai
  • Journal Title: Journal of Nanoscience and Nanotechnology 9 Pages: 386-390
  • Peer Reviewed
• [Journal Article] Preparation of dendrimer-loaded microcapsules by a layer-by-layer deposition of polyelectrolytes (2009)
  • Author(s): Tomita, Shigeru
  • Journal Title: Materials Science & Engineering C 29 Pages: 2024-2028
  • Peer Reviewed
• [Journal Article] Layer-by-layer assembled thin films composed of carboxyl-terminated poly (amidoamine) dendrimer as a pH-sensitive nano-device (2008)
  • Author(s): Shigeru Tomita, Katsuhiko Sato, Jun-ichi Anzai
  • Journal Title: Journal of Colloid and Interface Science 326 Pages: 35-40
  • Peer Reviewed
• [Journal Article] Preparation of layer-by-layer thin films containing insulin and its pH-sensitive decomposition (2008)
  • Author(s): Kentaro Yoshida, Hiroshi Sato, Shigehiro Takahashi, Jun-ichi Anzai
  • Journal Title: Polymer Journal 40 Pages: 90-91
  • NAID: 10021116024
  • Peer Reviewed
• [Journal Article] 交互累積法によるナノ薄膜およびナノカプセルを用いたバイオセンサ (2008)
  • Author(s): 王保珍, 江川祐哉, 佐藤勝彦, 安齋順一
  • Journal Title: マテリアルインテグレーション 21 Pages: 282-287
• [Journal Article] Layer-by-layer assembled thin films composed of carboxyl-terminated poly(amidoamine) dendrimer as a pH-sensitive nano-device (2008)
  • Author(s): Tomita, Shigeru
  • Journal Title: Journal of Colloid & Interface Science 325 Pages: 35-40
  • Peer Reviewed
• [Journal Article] Preparation of layer-by-layer thin films containing insulin and its pH-sensitive decomposition (2008)
  • Author(s): Yoshida, Kentaro
  • Journal Title: Polymer Journal 40 Pages: 90-91
  • Peer Reviewed
• [Journal Article] Effects of hydrogen peroxide on the electrochemical decomposition of layer-by-layer thin films composed of 2-iminobiotin-labeled poly(ethyleneimine)and avidin (2007)
  • Author(s): Sato, Katsuhiko
  • Journal Title: Journal of Colloid and Interface Science 315 Pages: 396-399
  • Peer Reviewed
• [Presentation] Sugar-sensitive films and microcapsules for sensing and release (2009)
  • Author(s): Jun-ichi Anzai
  • Organizer: 8^<th> Asian Conference on Chemical Sensors
  • Place of Presentation: Daegu
  • Year and Date: 2009-11-15
• [Presentation] pH-or glucose-sensitive thin films and microcapsules containing insulin (2009)
  • Author(s): Kentaro Yoshida, Jun-ichi Anzai
  • Organizer: 8^<th> Asian Conference on Chemical Sensors
  • Place of Presentation: Daegu
  • Year and Date: 2009-11-15
• [Presentation] Sugar-sensitive films and microcapsules for sensing and release (2009)
  • Author(s): Anzai, Jun-ichi
  • Organizer: 8^<th> Asian Conference on Chemical Sensors
  • Place of Presentation: Daegu, Korea
  • Year and Date: 2009-11-12
• [Presentation] 刺激応答性インスリン放出システムの開発 (2009)
  • Author(s): 吉田健太郎, 安齋順一
  • Organizer: 第48回日本薬学会東北支部大会
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-18
• [Presentation] 糖存在下におけるフェロセンボロン酸の電気化学応答 (2009)
  • Author(s): 安齋順一
  • Organizer: 第48回日本薬学会東北支部大会
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-18
• [Presentation] グルコースセンサ機能をもつインスリンカプセル (2009)
  • Author(s): 佐藤勝彦, 安齋順一
  • Organizer: 第48回化学センサ研究発表会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-10
• [Presentation] pH応答性インスリン放出カプセルの開発 (2009)
  • Author(s): 吉田健太郎, 安齋順一
  • Organizer: みちのく分析科学シンポジウム2009
  • Place of Presentation: 仙台
  • Year and Date: 2009-07-25
• [Presentation] グルコースオキシダーゼを固定化した微粒子およびカプセルの糖応答性の評価 (2009)
  • Author(s): 保科直, 安齋順一
  • Organizer: みちのく分析科学シンポジウム2009
  • Place of Presentation: 仙台
  • Year and Date: 2009-07-25
• [Presentation] Insulin-containing thin films and nano-particles for glucose-triggered controlled release (2008)
  • Author(s): Kentaro Yoshida, Jun-ichi Anzai
  • Organizer: The 3^<rd> International Symposium on Environmental Economy and Technology
  • Place of Presentation: 深谷
  • Year and Date: 2008-08-26
• [Presentation] Sugar-sensitive thin films composed of phenylboronic acid-modified dendrimers (2008)
  • Author(s): Satoshi Niina, Jun-ichi Anzai
  • Organizer: The 3^<rd> International Symposium on Environmental Economy and Technology
  • Place of Presentation: 深谷
  • Year and Date: 2008-08-26
• [Presentation] フェニルボロン酸修飾デンドリマーとポリビニルアルコールを用いた糖応答性薄膜 (2008)
  • Author(s): 新名聖, 安齋順一
  • Organizer: みちのく分析科学シンポジウム2008
  • Place of Presentation: 仙台
  • Year and Date: 2008-07-19
• [Presentation] コンカナバリンAを用いたミクロカプセルの調製と糖応答性 (2008)
  • Author(s): 佐藤勝彦, 安齋順一
  • Organizer: 第69回分析化学討論会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-05-15
• [Presentation] コンカナバリンAを用いたカプセルの調製と糖応答性 (2008)
  • Author(s): 佐藤勝彦
  • Organizer: 第69回分析化学討論会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-05-15
• [Presentation] pH応答性インスリン含有ミクロカプセルの調製 (2008)
  • Author(s): 吉田健太郎, 安齋順一
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-28
• [Presentation] 糖類の刺激でインスリンを放出するマイクロカプセルの調製 (2008)
  • Author(s): 児玉大祐, 安齋順一
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-28
• [Presentation] 交互累積方により作製したマイクロカプセルの透過性 (2008)
  • Author(s): 遠藤好弘, 安齋順一
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-28
• [Presentation] Insulin nano-films sensitive to pH and glucose (2007)
  • Author(s): Shigehiro Takahashi, Jun-ichi Anzai
  • Organizer: 7^<th> East Asia Conference on Chemical Sensors
  • Place of Presentation: シンガポール
  • Year and Date: 2007-12-03
• [Presentation] インスリンを含有する交互累積膜の調製と性質 (2007)
  • Author(s): 吉田健太郎, 安齋順一
  • Organizer: 2007高分子学会東北支部研究発表会
  • Place of Presentation: 秋田
  • Year and Date: 2007-11-15
• [Presentation] レクチンを構成成分とするミクロカプセルの糖応答性 (2007)
  • Author(s): 児玉大祐, 安齋順一
  • Organizer: みちのく分析科学シンポジウム2007
  • Place of Presentation: 仙台
  • Year and Date: 2007-07-27
• [Presentation] インスリン累積膜の調製とpH応答性 (2007)
  • Author(s): 吉田健太郎, 安齋順一
  • Organizer: みちのく分析科学シンポジウム2007
  • Place of Presentation: 仙台
  • Year and Date: 2007-07-27
• [Presentation] 交互累積膜によるナノ薄膜・ナノカプセルの機能開発 (2007)
  • Author(s): 安齋順一
  • Organizer: みちのく分析科学シンポジウム2007
  • Place of Presentation: 仙台
  • Year and Date: 2007-07-27