Development of efficient drug analysis system in forensic practice

• Project/Area Number: 19390185
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Kyushu University
• Principal Investigator: KUDO Keiko Kyushu University, 大学院・医学研究院, 講師 (10186405)
• Co-Investigator(Kenkyū-buntansha): IKEDA Noriaki 九州大学, 大学院・医学研究院, 教授 (60176097) ; TSUJI Akiko 九州大学, 大学院・医学研究院, 助教 (10171993)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2007: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
• Keywords: GC / MS / データベース / LC / NAGINATA / 薬毒物スクリーニング / フィンランド / GC/MS / スクリーニング / 農薬 / 酸性薬物 / 法医鑑定 / MSデータベース / 乱用薬物 / NAGNATA^<TM>

Research Abstract

A novel screening procedure using calibration locking databases was developed by GC/MS. The method does not require standard substances and semi-quantitative data can be obtained simultaneously. We also developed a rapid and quantitative screening method for 43 hypnotics by LC/MS. The attempt for developing the most efficient drug analysis system in Japan was made based on a retrospective study on the kinds of compounds causing poisonings and on the analysis of the frequency of their use employing two series of reports dealing with fatal poisoning cases and a review of the drug analysis system accompanied by forensic autopsy in Finland.

Research Products

• [Journal Article] Pattern of poisoning in Japan : selection of drugs and poisons for systematic toxicological analysis. (2010)
  • Author(s): K.Kudo, T.Ishida, W.Hikiji, Y.Usumoto, T.Umehara, K.Nagamatsu, A.Tsuji, N.Ikeda
  • Journal Title: Forensic Toxicol 28 Pages: 25-32
  • Peer Reviewed
• [Journal Article] Pattern of poisoning in Japan : selection of drugs and poisons for systematic toxicological analysis (2010)
  • Author(s): K.Kudo, et al.
  • Journal Title: Forensic Toxicology 28 Pages: 25-32
  • Peer Reviewed
• [Journal Article] Construction of calibration-locking databases for rapid and reliable drug screening by gas chromatography-mass spectrometry. (2009)
  • Author(s): K.Kudo, T.Ishida, W.Hikiji, M.Hayashida, K.Uekusa, Y.Usumoto, A.Tsuji, N.Ikeda
  • Journal Title: Forensic Toxicol 27 Pages: 21-31
  • Peer Reviewed
• [Journal Article] Rapid and quantitative screening method for 43 benzodiazepines and their metabolites, zolpidem and zopiclone in human plasma by liquid chromatography/mass spectrometry with a small particle column. (2009)
  • Author(s): T.Ishida, K.Kudo, M.Hayashida, N.Ikeda
  • Journal Title: J Chromatogr B 877 Pages: 2652-2657
  • NAID: 10028022601
  • Peer Reviewed
• [Journal Article] Construction of calibration-locking Databases for rapid and reliable drug screening by gas chromatography-mass spectrometory (2009)
  • Author(s): Kudo K, Ishida T, Hikiji W, Hayashida M, Uekusa K, Usumoto Y, Tsuji A, Ikeda N.
  • Journal Title: Forensic Toxicol. 27 Pages: 21-31
  • Peer Reviewed
• [Journal Article] Rapid and quantitative screening method for 43 benzodiazepines and their metabolites, zolpidem and zopiclone in human plasma by liquid chromatography/mass spectrometry with a small particle column (2009)
  • Author(s): T.Ishida, et al.
  • Journal Title: J Chromatography B 277 Pages: 2652-2657
  • Peer Reviewed
• [Journal Article] Construction of calibration-locking databases for rapid and reliable drugscreening by gas chromatography-mass spectrometry (2009)
  • Author(s): K. Kudo, et al.
  • Journal Title: Forensic Toxicology 27 Pages: 21-31
  • Peer Reviewed
• [Journal Article] フィンランドにおける法医解剖に伴う薬物分析システムの調査研究 (2008)
  • Author(s): 工藤恵子, 石田知己, 井上裕匡, 影浦光義
  • Journal Title: 日本法医学雑誌 62 Pages: 124-135
  • NAID: 40016352763
  • Peer Reviewed
• [Journal Article] ガスクロマトブラフィー質量分析による標準物質を必要としない乱用薬物一斉分析法の開発 (2008)
  • Author(s): 工藤恵子, 石田知己, 池田典昭
  • Journal Title: J Mass Spectrom Soc Jpn 56 Pages: 123-130
  • Peer Reviewed
• [Journal Article] フィンランドにおける法医解剖に伴う薬物分析システムの調査研究 (2008)
  • Author(s): 工藤 恵子, et al.
  • Journal Title: 日本法医学雑誌 62 Pages: 124-135
  • NAID: 40016352763
  • Peer Reviewed
• [Journal Article] Rapid diagnosis of drug intoxication using novel NAGINATA gas chromatography/mass spectrometry software. (2007)
  • Author(s): T.Ishida, K.Kudo, S.Naka, K.Toubou, T.Noguchi, N.Ikeda
  • Journal Title: Rapid Commun Mass Spectrom 21 Pages: 3129-3138
  • Peer Reviewed
• [Journal Article] Rapid diagnosis of drug intoxication using novel NAGINATA gas chromatography/mass spectrometry software (2007)
  • Author(s): Ishida T, Kudo K, Naka S, Toubou K, Noguchi T, Ikeda N.
  • Journal Title: Rapid Commun Mass Spectrom 21 Pages: 3129-3138
  • Peer Reviewed
• [Journal Article] Simultaneonus determination of 13 amphetamine related drugs in human whole blood using an enhanced polymer column and gas chromatography-mass spectrometry (2007)
  • Author(s): Kudo K, Ishida T, Hara K, Kashimura S, TsujiA, Ikeda N.
  • Journal Title: J Chromatography B 855 Pages: 115-120
  • Peer Reviewed
• [Journal Article] Rapid diagnosis of drug intoxication using novel NAGINATATM gas chromatography/mass spectrometry software (2007)
  • Author(s): T. Ishida, et. al.
  • Journal Title: Rapid Communication in Maes Spectrometry 21 Pages: 3129-3138
  • Peer Reviewed
• [Journal Article] Simultaneous determination of 13 amphetamine related drugs in human whole blood using enhanced polymer column and gas chromatography-mass spectrometry (2007)
  • Author(s): K. Kudo, et. al.
  • Journal Title: Journal of Chromatography B 855 Pages: 115-120
  • Peer Reviewed
• [Presentation] NAGINATAデータベース法による非ステロイド性抗炎症薬のスクリーニング (2010)
  • Author(s): 永松久実
  • Organizer: 日本法中毒学会第29年会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-24
• [Presentation] NAGINATAデータベース法による非ステロイド性抗精神薬のスクリーニング (2010)
  • Author(s): 永松久実, 他
  • Organizer: 日本法中毒学会第29年会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-24
• [Presentation] 我が国における中毒発生状況:体系的薬毒物分析のための中毒起因物質の選定 (2010)
  • Author(s): 工藤恵子
  • Organizer: 第94次日本法医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-24
• [Presentation] Construction of calibration locking databases for rapid and reliable drug screening by gas chromatography-mass spectrometry (2009)
  • Author(s): 工藤恵子
  • Organizer: 第47回国際法中毒学会
  • Place of Presentation: ジュネーブ
  • Year and Date: 2009-08-24
• [Presentation] Construction of calibration locking databases for rapid and reliable drug screening by gas chromatography-mass spectrometry (2009)
  • Author(s): Kudo K, Hikiji W, Hayashida M, Uekusa K, Ikeda N.
  • Organizer: The International Association of Forensic Toxicologists 47^<th> International meeting
  • Place of Presentation: Geneve
  • Year and Date: 2009-08-24
• [Presentation] GC/MSによる標準品を必要としない薬毒物スクリーニングのためのデータベースの構築 (2009)
  • Author(s): 工藤恵子
  • Organizer: 日本法中毒学会第28年会
  • Place of Presentation: 金沢
  • Year and Date: 2009-06-13
• [Presentation] 標準物質を必要としない簡易薬毒物スクリーニング法の開発と臨床応用の可能性 (2009)
  • Author(s): 工藤恵子
  • Organizer: 第1回 日本中毒学会九州地方会
  • Place of Presentation: 飯塚
  • Year and Date: 2009-03-07
• [Presentation] UPLC-MSによる血漿中ベンゾジアゼピン系薬物およびその代謝物、ゾルピデム、ゾピクロン計43種の迅速高感度定量法の確立 (2008)
  • Author(s): 石田知己
  • Organizer: 第33回 日本医用マススペクトル学会年会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-26
• [Presentation] UPLC-MSによる血漿中ベンゾジアゼピン系薬物およびその代謝物、ゾルピデム、ゾピクロン計43種の迅速高感度定量法の確立 (2008)
  • Author(s): 石田 知己, 他
  • Organizer: 第33回 日本医用マススペクトル学会年会
  • Place of Presentation: 飯塚
  • Year and Date: 2008-09-26
• [Presentation] GC/MSによる薬毒物スクリーニングにおけるアセチル化の有用性 (2008)
  • Author(s): 工藤恵子
  • Organizer: 日本法中毒学会第27年会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-14
• [Presentation] GC/MSによる薬毒物スクリーニングにおけるアセチル化の有用性 (2008)
  • Author(s): 工藤 恵子, 他
  • Organizer: 日本法中毒学会 第27年会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-14
• [Presentation] 標準物質を必要としない乱用薬物一斉分析法の開発に向けて (2007)
  • Author(s): 工藤 恵子, 他
  • Organizer: BMSコンファレンス
  • Place of Presentation: 三重県鳥羽市
  • Year and Date: 2007-07-10
• [Remarks] ガスクロマトグラフィー・質量分析用薬毒物データベース