How heat treatment reinforces human dentin in pulpless teeth

• Project/Area Number: 19390482
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: HAYASHI Mikako Osaka University, 歯学部・附属病院, 講師 (40271027)
• Co-Investigator(Kenkyū-buntansha): 岩見 行晃 大阪大学, 大学院・歯学研究科, 助教 (90303982) ; 菅田 淳 広島大学, 工学(系)研究科, 教授 (60162913)
• Co-Investigator(Renkei-kenkyūsha): 菅田 淳 広島大学, 大学院・工学研究科, 教授 (60162913)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2009: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2008: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000); Fiscal Year 2007: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: 保存修復学 / 象牙質 / 機械的強度 / 破折 / コラーゲン / 顕微赤外分光分析 / 分子間架橋 / 高速液体クロマトグラフィー / 歯学 / ナノインデンテーション / エックス線回折 / 顕微ラマン分光分析 / 機械材料・材料力学 / 破面観察

Research Abstract

The strengthening effects of human dentin by heating were investigated with an aim of preventing tooth fracture. We found that a heat treatment with temperature at 110 to 140℃ was significantly effective to improve the mechanical strengths of dentin with showing anisotolopy. The strengthening mechanism of dentin by heating was found to be the compaction of dentin structure promoted by shrinking its collagen network.

Research Products

• [Journal Article] Effects of rehydration on dentin strengthened by heating or UV irradiation. (2010)
  • Author(s): Hayashi M, Okamura K, Koytchev EV, Furuya Y, Sugeta A, Ota T, Ebisu S
  • Journal Title: Journal of Dental Research 89 Pages: 154-158
  • Peer Reviewed
• [Journal Article] Effects of rehydration on dentin strengthened by heating or UV irradiation (2010)
  • Author(s): Hayashi, et al.
  • Journal Title: Journal of Dental Research 89 Pages: 154-158
  • Peer Reviewed
• [Journal Article] Effects of light penetration and smear layer removal on adhesion of post-cores to root canal dentin by self-etching adhesives. (2009)
  • Author(s): Wu H, Hayashi M, Okamura K, Koytchev EV, Imazato S, Tanaka S, Tanaka Y, Sano H, Ebisu S
  • Journal Title: Dental Materials 25 Pages: 1484-1492
  • Peer Reviewed
• [Journal Article] eaching of posterior composites in dental schools in Japan. (2009)
  • Author(s): Hayashi M, Seow LL, Lynch CD, Wilson NHF
  • Journal Title: Journal of Oral Rehabilitation 36 Pages: 292-298
  • Peer Reviewed
• [Journal Article] 加熱による象牙質の強化 (2009)
  • Author(s): 林美加子
  • Journal Title: The Journal of Dental Engineering 169 Pages: 17-20
  • Peer Reviewed
• [Journal Article] 恵比須繁之. 新規う蝕検知液Caries CheckRによるう蝕除去の客観性 (2009)
  • Author(s): 岩見行晃, 山本洋子, 永山智崇, 成田寛子
  • Journal Title: 日本歯科保存学雑誌 52 Pages: 384-392
  • Peer Reviewed
• [Journal Article] Teaching of posterior composites in dental schools in Japan (2009)
  • Author(s): Hayashi, et al.
  • Journal Title: Journal of Oral Rehabilitation 36 Pages: 292-298
  • Peer Reviewed
• [Journal Article] Development of a system for measuring fluoride distribution in teeth using a nuclear reaction. (2009)
  • Author(s): Yamamoto, Iwami, et al.
  • Journal Title: International Journal of PIXE 19 Pages: 91-102
  • Peer Reviewed
• [Journal Article] 新規う蝕検知液Caries Check[○!R]によるう蝕除去の客観性 (2009)
  • Author(s): 岩見行晃, 他
  • Journal Title: 日本歯科保存学雑誌 52 Pages: 384-392
  • Peer Reviewed
• [Journal Article] Effects of light penetration and smear layer removal on edhesion of post-cores to root canal dentin by self-etching adhesives. (2009)
  • Author(s): Wu, Hayashi, et al.
  • Journal Title: Dental Materials 25 Pages: 1484-1492
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 林美加子
  • Journal Title: エンド難症例 メカニズムと臨床対応-支台築造と歯根破折-(恵比須繁之編)(医歯薬出版) Pages: 153-161
• [Journal Article] Teaching pf posterior composites in dental schools in Japan (2009)
  • Author(s): Hayashi, et al
  • Journal Title: Journal of Oral Rehabilitation
  • Peer Reviewed
• [Journal Article] Heat treatment strengthens human dentin. (2008)
  • Author(s): Hayashi M, Koytchev EV, Okamura K, Sugeta A, Hongo C, Okuyama K, Ebisu S
  • Journal Title: Journal of Dental Research 87 Pages: 762-766
  • Peer Reviewed
• [Journal Article] The root canal bonding of chemical-cured total-etch resin cement. (2008)
  • Author(s): Hayashi M, Okamura K, Wu H, Takahashi Y, Koytchev EV, Imazato S, Ebisu S
  • Journal Title: Journal of Endodontics 34 Pages: 583-586
  • Peer Reviewed
• [Journal Article] Static and fatigue fracture resistances of pulpless teeth restored with post-cores. (2008)
  • Author(s): Hayashi M, Sugeta A, Takahashi Y, Ebisu S
  • Journal Title: Dental Materials 24 Pages: 1178-1186
  • Peer Reviewed
• [Journal Article] Relationship between the color of carious dentin with varying lesion activity, and bacterial detection. (2008)
  • Author(s): Iwami Y, Hayashi N, Ebisu S
  • Journal Title: Journal of Dentistry 36 Pages: 143-151
• [Journal Article] Heat treatment strengthens human dentin (2008)
  • Author(s): Hayashi M., et al
  • Journal Title: Journal of Dental Research 87 Pages: 762-766
  • Peer Reviewed
• [Journal Article] The root canal bonding of chemical-cured total-etch resin cement (2008)
  • Author(s): Hayashi M., et al
  • Journal Title: Journal of Endodontics 54 Pages: 583-586
  • Peer Reviewed
• [Journal Article] Static and fatigue fracture resistances of pulpless teethrestored with post-cores (2008)
  • Author(s): Hayashi M., et al
  • Journal Title: Dental Materials 24 Pages: 1178-1186
  • Peer Reviewed
• [Journal Article] Key factors in achieving firm adhesion in post-core restorations (2008)
  • Author(s): Hayashi M., et al
  • Journal Title: Dentistry in Japan 44 Pages: 22-28
  • NAID: 10023922374
• [Journal Article] Relationship between the color of carious dentin with varying lesion activity, and bacterial detection (2008)
  • Author(s): Iwami Y, et al
  • Journal Title: Journal of Dentistry 36(in press) Pages: 143-151
  • Peer Reviewed
• [Journal Article] Heat treatment strengthens human dentin (2008)
  • Author(s): Hayashi M.
  • Journal Title: Journal of Dental Research (in press)
  • Peer Reviewed
• [Journal Article] The root canal bonding of chemical-cured total-etch resin cement (2008)
  • Author(s): Hayashi M.
  • Journal Title: Journal of Endodontics (in press)
  • Peer Reviewed
• [Journal Article] Static and fatigue fracture resistances of pulpless teeth restored with post-cores (2008)
  • Author(s): Hayashi M.
  • Journal Title: Dental Materials (in press)
  • Peer Reviewed
• [Journal Article] Relationship between the color of carious dentin with varying lesion activity, and bacterial detection (2008)
  • Author(s): Iwami Y.
  • Journal Title: Journal of Dentistry 36 Pages: 143-151
  • Peer Reviewed
• [Journal Article] Key factors in achieving firm adhesion in post-core restorations (2008)
  • Author(s): Hayashi M.
  • Journal Title: Dentistry in Japan (in press)
  • NAID: 10023922374
• [Journal Article] Evaluating the objectivity of caries removal with a caries detector dye using color evaluation and PCR. (2007)
  • Author(s): Iwami Y, Hayashi N, Yamamoto H, Hayashi M, Takeshige F, Ebisu S
  • Journal Title: Journal of Dentistry 35 Pages: 749-754
  • Peer Reviewed
• [Journal Article] Evaluating the objectivity of caries removal with a caries detector dye using color evaluation and PCR (2007)
  • Author(s): Iwami Y.
  • Journal Title: Journal of Dentistry 35 Pages: 749-754
  • Peer Reviewed
• [Presentation] 失活歯強化法の臨床応用を目指した加熱および紫外線による象牙質強化メカニズムの解明 (2009)
  • Author(s): 林美加子
  • Organizer: 大阪大学歯学会第109回例会 平成21年度弓倉学術奨励賞受賞講演
  • Place of Presentation: 吹田市
  • Year and Date: 2009-11-12
• [Presentation] 歯内療法学実習に使用する人工歯と顎模型の開発. (2009)
  • Author(s): 辻本恭久, 林美加子, 他
  • Organizer: 第28回日本歯科医学教育学会
  • Place of Presentation: 広島市
  • Year and Date: 2009-11-06
• [Presentation] 紫外線照射による象牙質強化メカニズムの解析 (2009)
  • Author(s): 古谷優, 林美加子, 恵比須繁之
  • Organizer: 第131回日本歯科保存学会
  • Place of Presentation: 仙台国際センター(仙台市)
  • Year and Date: 2009-10-30
• [Presentation] 紫外線照射による象牙質強化メカニズムの解析 (2009)
  • Author(s): 古谷優, 林美加子, 他
  • Organizer: 第131回日本歯科保存学会
  • Place of Presentation: 仙台市
  • Year and Date: 2009-10-30
• [Presentation] 赤外線サーモグラフィを用いた歯根破折診断法の開発-チップ形態の差異による摩擦熱発生条件についての検討- (2009)
  • Author(s): 徳川真美, 林美加子, 他
  • Organizer: 第131回日本歯科保存学会
  • Place of Presentation: 仙台市
  • Year and Date: 2009-10-30
• [Presentation] 歯内療法の使用器具薬剤に関するアンケート結果 第2報:大学診療室. (2009)
  • Author(s): 山内由美, 林美加子, 他
  • Organizer: 第131回日本歯科保存学会
  • Place of Presentation: 仙台市
  • Year and Date: 2009-10-29
• [Presentation] A study on diagnotic method of root fracture by infrared thermography-condition of frictional heat generation- (2009)
  • Author(s): Tokugawa, Hayashi, et al.
  • Organizer: The 44th meeting of the LADR-Continental European Division
  • Place of Presentation: Munich, Germany
  • Year and Date: 2009-09-12
• [Presentation] 骨質・骨構造と骨粗鬆症-歯・象牙質の強度とコラーゲン (2009)
  • Author(s): 林美加子
  • Organizer: 第27回日本骨代謝学会シンポジウム
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2009-07-24
• [Presentation] 骨質・骨構造と骨粗鬆症-歯・象牙質の強度とコラーゲン (2009)
  • Author(s): 林美加子
  • Organizer: 第27回日本骨代謝学会シンポジウム
  • Place of Presentation: 大阪市
  • Year and Date: 2009-07-24
• [Presentation] 根完成歯の自家移植の予後に影響を及ぼす因子について (2009)
  • Author(s): 三宅直子, 林美加子, 他
  • Organizer: 第130回日本歯科保存学会
  • Place of Presentation: 札幌市
  • Year and Date: 2009-06-11
• [Presentation] 定期的メインテナンスはう蝕病変を抑制するか? (2009)
  • Author(s): 伊藤中, 林美加子, 他
  • Organizer: 第130回日本歯科保存学会
  • Place of Presentation: 札幌市
  • Year and Date: 2009-06-11
• [Presentation] 歯内療法の使用器具薬剤に関するアンケート結果 第1報:大学基礎実習. (2009)
  • Author(s): 小澤寿子, 林美加子, 他
  • Organizer: 第130回日本歯科保存学会
  • Place of Presentation: 札幌市
  • Year and Date: 2009-06-11
• [Presentation] Questionnaire conducted Japanese dental schools for endodontic instruments and medicine for pre-clinical practice. (2009)
  • Author(s): 林美加子
  • Organizer: 日本補綴歯科学会第118回学術大会シンポジウム
  • Place of Presentation: 京都市
  • Year and Date: 2009-06-06
• [Presentation] Questionnaire conducted Japanese dental schools for endodontic instruments and medicine for pre-clinical practice. (2009)
  • Author(s): Ozawa, Hayashi, et al.
  • Organizer: The 15th Scientific Meeting and the 12^<th> Biennial General Meeting of the Asian Pacific Endodontic Confederation.
  • Place of Presentation: Tokyo
  • Year and Date: 2009-04-25
• [Presentation] 赤外線サーモグラフィーを用いた歯根破折診断法の開発 一摩擦熱発生条件についての検討- (2008)
  • Author(s): 徳川真美, 岩見行晃, 林美加子, 他
  • Organizer: 日本歯科保存学会平成20年度秋季大会
  • Place of Presentation: 富山国際会議場
  • Year and Date: 2008-11-08
• [Presentation] Effects of heating on the flexural strength of human dentin. (2008)
  • Author(s): Hayashi, M., Koytchev, E.V., Okamura, K., Ebisu, S.
  • Organizer: The 86th General Session of the IADR
  • Place of Presentation: Metro Toronto Convention Centre (Toronto, Canada)
  • Year and Date: 2008-07-04
• [Presentation] Effects of heating on the flexural strength of human dentin (2008)
  • Author(s): Hayashi M, et al
  • Organizer: International Association for Dental Research86^<th> General Session and Exhibition
  • Place of Presentation: Toronto
  • Year and Date: 2008-07-02
• [Presentation] 各種う蝕検知液によるう蝕除去の客観性 (2008)
  • Author(s): 岩見 行晃, 他
  • Organizer: 日本歯科保存学会平成20年度春季大会
  • Place of Presentation: 朱鷺メッセ(新潟コンベンシヨンセンター)
  • Year and Date: 2008-06-05
• [Presentation] A comparison of bond strength of fiber po sts luted with different resin cements. (2008)
  • Author(s): 呉紅霞, 林美加子, 岡村健司, Koytchev EV, 今里聡, 恵比須繁之
  • Organizer: International Symposium for Adhesive De ntistry
  • Place of Presentation: 金沢アートホール(金沢市)
  • Year and Date: 2008-02-16
• [Presentation] A comparison of bond strength of fiber posts luted with different resin cements (2008)
  • Author(s): 呉 紅霞, 林 美加子, 他
  • Organizer: International Symposium for Adhesive Dentistry
  • Place of Presentation: 金沢アートホール
  • Year and Date: 2008-02-16
• [Presentation] 加熱による象牙質強化メカニズムの解析 (2007)
  • Author(s): 岡村健司, 林美加子, Koytchev, E.V., 恵比須繁之
  • Organizer: 第127回日本歯科保存学会秋季大会
  • Place of Presentation: 岡山コンベンションセンター(岡山市)
  • Year and Date: 2007-11-08
• [Presentation] 加熱による象牙質強化メカニズムの解析 (2007)
  • Author(s): 岡村 健司 林 美加子, 他
  • Organizer: 日本歯科保存学会平成19年度秋季大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2007-11-08
• [Presentation] The effect of light penetration on the adhesive properties of post-core materials to root canal dentin (2007)
  • Author(s): 呉 紅霞, 林 美加子, 他
  • Organizer: 日本歯科保存学会平成19年度秋季大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2007-11-08
• [Presentation] 象牙質接着システムおよびレジンセメントの根管象牙質への接着強さ (2007)
  • Author(s): 岡村 健司, 林 美加子, 他
  • Organizer: 第25回日本歯科理工学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2007-05-12
• [Book] エンド難症例メカニズムと臨床対応,-支台築造と歯根破折- (2009)
  • Author(s): 林美加子, 恵比須繁之(編)
  • Publisher: 医歯薬出版
• [Patent(Industrial Property Rights)] 歯科装置 (2009)
  • Inventor(s): 林美加子, 恵比須繁之
  • Industrial Property Rights Holder: 大阪大学
  • Industrial Property Number: 2009-207783
  • Filing Date: 2009-09-09
• [Patent(Industrial Property Rights)] 歯科装置 (2009)
  • Inventor(s): 林美加子 恵比須繁之
  • Industrial Property Rights Holder: 林美加子 恵比須繁之
  • Industrial Property Number: 2009-207783
  • Filing Date: 2009-09-09
• [Patent(Industrial Property Rights)] 歯科装置 (2007)
  • Inventor(s): 林美加子, 恵比須繁之, 菅田淳
  • Industrial Property Rights Holder: 大阪大学,広島大学
  • Filing Date: 2007-04-13
• [Patent(Industrial Property Rights)] 歯科装置 (2007)
  • Inventor(s): 林美加子, 他
  • Industrial Property Rights Holder: 大阪大学
  • Filing Date: 2007-04-13
  • Overseas