Development of alveolar bone regenerative medicine using regulated autologous cells

• Project/Area Number: 19390497
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 補綴理工系歯学
• Research Institution: Hiroshima University
• Principal Investigator: NISHIMURA Masahiro Hiroshima University, 大学院・医歯薬学総合研究科, 准教授 (00294570)
• Co-Investigator(Kenkyū-buntansha): KATO Yukio 広島大学, 大学院・医歯薬学総合研究科, 教授 (10112062) ; SADAMORI Shinsuke 広島大学, 大学院・医歯薬学総合研究科, 准教授 (40187167)
• Research Collaborator: SAKAI Yuuhiro 株式会社ジーシー, 研究員 ; SUEHIRO Fumio 広島大学, 大学院・医歯薬学総合研究科 ; TSUBOI Masahiro 広島大学, 大学院・医歯薬学総合研究科 ; KAMADA Kohichi 広島大学, 大学院・医歯薬学総合研究科
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2009: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2008: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2007: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: 再生医療 / 骨再生 / 幹細胞 / 間葉系幹細胞 / 歯槽骨再生 / 骨増生

Research Abstract

We have studied basic area how to augment alveolar ridge by minimum intervention using autologous alveolar bone derived mesenchymal stem cells (MSC). We successfully collected MSC from alveolar bone and expanded them in vitro. We have found MSC specific gene and osteogenesis marker. We have found that β-tricalcium phosphates as the suitable scaffold combined with MSC for bone augmentation. Taken together、we have found several basic findings for future bone tissue engineering medicine.

Research Products

• [Journal Article] Identification of mesenchymal stem cell (MSC)-transcription factors by microarray and knockdown analyses (2009)
  • Author(s): Kubo H, Shimizu M, Taya Y, Kawamoto T, Michida M, Kaneko E, Igarashi A, Nishimura M, Segoshi K, Shimazu Y, Tsuji K, Aoba T, Kato Y
  • Journal Title: and signature molecule-marked MSC in bone marrow by immunohistochemistry: Genes Cells 14 Pages: 407-424
• [Journal Article] Identification of mesenchymal stem cell (MSC)-transcription factors by microarray and knockdown analyses, and signature molecule-marked MSC in bone marrow by immunohistochemistry (2009)
  • Author(s): Kubo H
  • Journal Title: Genes Cells 14 Pages: 407-424
  • Peer Reviewed
• [Journal Article] Enhancement of osteogenesis by concanavalin A in human bone marrow mesenchymal stem cell cultures (2008)
  • Author(s): Sekiya K, Nishimura M, Suehiro F, Nishimura H, Hamada T, Kato Y
  • Journal Title: Int J Art Org 31 Pages: 708-715
  • NAID: 120001092432
• [Journal Article] Enhancement of osteogenesis by concanavalin A in human bone marrow mesenchymal stem cell cultures (2008)
  • Author(s): Sekiya K
  • Journal Title: Int J Art Org 31 Pages: 708-715
  • Peer Reviewed
• [Presentation] 顎骨骨髄由来間葉系幹細胞採取法のクリティカルパラメータ (2010)
  • Author(s): 西村正宏
  • Organizer: 第9回日本再生医療学会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-19
• [Presentation] 骨増生に向けた顎骨骨髄由来間質細胞の確実な培養法の開発 (2010)
  • Author(s): 西村正宏
  • Organizer: H22年度日本口腔インプラント学会九州支部学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2010-02-28
• [Presentation] Alveolar ridge augmentation using expaned alveolar bone marrow derived stromal cells (2010)
  • Author(s): Nishimura M
  • Organizer: 2nd International Workshop on BioDental Education & Research Hiroshima University Graduate School of Biomedical Sciences 2010
  • Place of Presentation: Hiroshima
  • Year and Date: 2010-02-12
• [Presentation] Alveolar ridge augmentation using expaned alveolar bone marrow derived stromal cells (2010)
  • Author(s): Nishimura M
  • Organizer: 2^<nd> International Workshop on BioDental Education & Research Hiroshima University Graduate School of Biomedical Sciences 2010
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 2010-02-12
• [Presentation] 間葉系幹細胞の骨分化に及ぼすリン酸カルシウム系Scaffoldの影響 (2009)
  • Author(s): 西村正宏
  • Organizer: H21年度日本補綴歯科学会九州支部学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-10-11
• [Presentation] 間葉系幹細胞の骨分化に及ぼすリン酸カルシウム系Scaffoldの影響 (2009)
  • Author(s): 西村正宏
  • Organizer: H21年度日本補綴歯科学会九州支部学術大会(福岡)
  • Place of Presentation: 福岡
  • Year and Date: 2009-10-11
• [Presentation] Interface、implant、regenerated bone and recipient alveolar bone (2009)
  • Author(s): Nishimura M
  • Organizer: The 3rd International Symposium for Interface Oral Health Science、2009
  • Place of Presentation: Sendai
  • Year and Date: 2009-01-16
• [Presentation] Interface, implant, regenerated bone and recipient alveolar bone (2009)
  • Author(s): Nishimura M
  • Organizer: The 3rd International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2009-01-16
• [Presentation] Alveolar ridge augmentation using alveolar bone mesenchymal stem cells (2008)
  • Author(s): Nishimura M
  • Organizer: Academy of Prosthetic and Regenerative Sciences
  • Place of Presentation: Nagoya
  • Year and Date: 2008-11-28
• [Presentation] Alveolar ridge augmentation using alveolar bone mesenchymal stem cells (2008)
  • Author(s): Nishimura M
  • Organizer: Academy of Prosthetic and Regenerative Sciences
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2008-11-28
• [Presentation] 自己間葉系幹細胞を用いた低侵襲な顎堤増生法の開発 (2008)
  • Author(s): 坪井将洋
  • Organizer: 第38回日本口腔インプラント学会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-13
• [Presentation] 顎骨由来の間葉系幹細胞を用いた骨増生法の有効性 (2008)
  • Author(s): 坪井将洋
  • Organizer: 平成20年度日本補綴歯科学会九州・中国・四国支部合同学術大会
  • Place of Presentation: 別府
  • Year and Date: 2008-08-30
• [Presentation] Strict selection of transcription factors involved in mesenchymal stem ce11s osteogenesis (2008)
  • Author(s): Suehiro F
  • Organizer: 86th IADR
  • Place of Presentation: Totonto, Canada
  • Year and Date: 2008-07-02
• [Presentation] 間葉系幹細胞の骨分化に関わる転写調節関連遺伝子の探索 (2008)
  • Author(s): 末廣史雄
  • Organizer: 第117回日本補綴歯科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-06-08
• [Presentation] 歯肉線維芽細胞と間葉系幹細胞の遊走に及ぼすトロンビンの作用機序の解析 (2008)
  • Author(s): 坪井将洋
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-03-13
• [Book] Interface、implant、regenerated bone and recipient alveolar bone (2010)
  • Author(s): Nishimura M, Sakai Y Suehiro F, Tsuboi M, Kamada K, Hori T, Sakai M, Takeda M, Tsuji K, Hamada T
  • Total Pages: 435
  • Publisher: Springer
• [Book] Interface Oral Health Science 2009 (2010)
  • Author(s): Nishimura M
  • Total Pages: 4
  • Publisher: Springer
• [Remarks]
  • URL: http://www.dh.nagasaki-u.ac.jp/pros2/Nishimura%20Top.html
• [Remarks]
  • URL: http://prc.hiroshima-u.ac.jp/project/shousai.phpjprojec_id=25&pageMode=top
• [Patent(Industrial Property Rights)] 誘導性間葉系幹細胞およびその作製方法 (2009)
  • Inventor(s): 加藤幸夫, 河本健, 西村正宏, 辻紘一郎
  • Industrial Property Rights Holder: 国立大学法人広島大学、株式会社ツーセル
  • Industrial Property Number: 2009-115378
  • Filing Date: 2009-05-12
• [Patent(Industrial Property Rights)] 間葉系幹細胞の骨化及び/又は軟骨化促進剤と骨化及び/又は軟骨化促進方法 (2009)
  • Inventor(s): 加藤幸夫, 西村正宏, 西村春樹, 辻紘一郎, 関谷健祐
  • Industrial Property Rights Holder: (株)ツーセル、加藤幸夫
  • Filing Date: 2009-10-09
• [Patent(Industrial Property Rights)] 誘導性間葉系幹細胞およびその作製方法 (2009)
  • Inventor(s): 加藤幸夫
  • Industrial Property Rights Holder: 広島大学
  • Industrial Property Number: 2009-115378
  • Filing Date: 2009-05-12