Selection of malaria protective sera useful for novel vaccine candidate discovery

• Project/Area Number: 19406009
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Ehime University
• Principal Investigator: TSUBOI Takafumi Ehime University, 無細胞生命科学工学研究センター, 教授 (00188616)
• Co-Investigator(Kenkyū-buntansha): TAKEO Satoru 愛媛大学, 無細胞生命科学工学研究センター, 講師 (40302666)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2007: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 感染症 / マラリア / ワクチン / ゲノム / タイ / バイオテクノロジー / 国際研究者交流

Research Abstract

The individuals live in malaria endemic area who repeatedly exposed to the malaria parasite gradually develop immunity to the clinical manifestations of infection. This resistance to clinical disease is partly mediated by antibodies against the asexual blood stages of the malaria parasite. To identify such individuals harboring protective antibodies we have screened the human serum specimen useful for the future vaccine candidate discovery. After three-years surveillance in the endemic village in western Thailand, we have successfully obtained both asymptomatic sera and symptomatic patient sera as negative controls.

Research Products

• [Journal Article] Wheat germ cell-free technology for accelerating the malaria vaccine research. (2009)
  • Author(s): Takeo S, Arumugam TU, Torii M, Tsuboi T.
  • Journal Title: Expert Opin Drug Discov. 4(in press) Pages: 1191-1199
  • Peer Reviewed
• [Journal Article] Malaria ookinete surface protein-based vaccination via the intranasal route completely blocks parasite transmission in both passive and active vaccination regimens in a rodent model of malaria infection. (2009)
  • Author(s): Arakawa T, Tachibana M, Miyata T, Harakuni T, Kohama H, Matsumoto Y, Tsuji N, Hisaeda H, Stowers A, Torii M, Tsuboi T.
  • Journal Title: Infect Immun 77 Pages: 5496-5500
  • Peer Reviewed
• [Journal Article] Evidence for the transmission of Plasmodium vivax in the Republic of the Congo, west central Africa. (2009)
  • Author(s): Culleton R, Ndounga M, Zeyrek FY, Coban C, Casimiro PN, Takeo S, Tsuboi T, Yadava A, Carter R, Tanabe K.
  • Journal Title: J Infect Dis. 200 Pages: 1465-1469
  • NAID: 120006983581
  • Peer Reviewed
• [Journal Article] Sterile protection against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: comparison of heterologous prime boost strategies. (2009)
  • Author(s): Jiang G, Shi M, Conteh S, Richie N, Banania G, Geneshan H, Valencia A, Singh P, Aguiar J, Limbach K, Kamrud KI, Rayner J, Smith J, Bruder JT, King CR, Tsuboi T, Takeo S, Endo Y, Doolan DL, Richie TL, Weiss WR.
  • Journal Title: PLoS ONE 4(8)
  • Peer Reviewed
• [Journal Article] Preerythrocytic, live-attenuated Plasmodium falciparum vaccine candidates by design. (2009)
  • Author(s): VanBuskirk KM, O'Neill MT, De La Vega P, Maier AG, Krzych U, Williams J, Dowler MG, Sacci, Jr. JB, Kangwanrangsan N, Tsuboi T, Kneteman NM, Heppner, Jr. DG, Murdock BA, Mikolajczak SA, Aly ASI, Cowman AF Kappe SHI.
  • Journal Title: Proc Natl Acad Sci U S A. 106 Pages: 13004-13009
  • Peer Reviewed
• [Journal Article] Enzymatic characterization of the Plasmodium vivax chitinase, a potential malaria transmission-blocking target. (2009)
  • Author(s): Takeo S, Hisamori D, Matsuda S, Vinetz J, Sattabongkot J, Tsuboi T.
  • Journal Title: Parasitol Int. 58 Pages: 243-248
  • NAID: 10027669617
  • Peer Reviewed
• [Journal Article] Single amino acid substitution in Plasmodium yoelii erythrocyte ligand determines its localization and controls parasite virulence. (2009)
  • Author(s): Otsuki H, Kaneko O, Thongkukiatkul A, Tachibana M, Iriko H, Takeo S, Tsuboi T, Torii M.
  • Journal Title: Proc Natl Acad Sci U S A. 106 Pages: 7167-7172
  • NAID: 120006984096
  • Peer Reviewed
• [Journal Article] A small-scale systematic analysis of alternative splicing in Plasmodium falciparum. (2009)
  • Author(s): Iriko H, Jin L, Kaneko O, Takeo S, Han ET, Tachibana M, Otsuki H, Torii M, Tsuboi T.
  • Journal Title: Parasitol Int. 58 Pages: 196-199
  • NAID: 10027669225
  • Peer Reviewed
• [Journal Article] Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast. (2009)
  • Author(s): Maeda T, Saito T, Harb OS, Roos DS, Takeo S, Suzuki H, Tsuboi T, Takeuchi T, Asai T.
  • Journal Title: Parasitol. Int. 58 Pages: 101-105
  • NAID: 10025330344
  • Peer Reviewed
• [Journal Article] Rhoptry neck protein RON2 forms a complex with microneme protein AMA1 in Plasmodium falciparum merozoites. (2009)
  • Author(s): Cao J, Kaneko O, Thongkukiatkul A, Tachibana M, Otsuki H, Gao Q, Tsuboi T, Torii M.
  • Journal Title: Parasitol Int. 58 Pages: 29-35
  • NAID: 10025329950
  • Peer Reviewed
• [Journal Article] Single amino acid substitution in Plasmodium yoelii erythrocyte ligand determines its localization and controls parasite virulence (2009)
  • Author(s): Otsuki H
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 7167-7172
  • Peer Reviewed
• [Journal Article] A small-scale systematic analysis of alternative splicing in Plasmodium falciparum (2009)
  • Author(s): Iriko H
  • Journal Title: Parasitol Int 58 Pages: 196-199
  • Peer Reviewed
• [Journal Article] Wheat germ cell-free technology for accelerating the malaria vaccine research (2009)
  • Author(s): Takeo S
  • Journal Title: Expert Opin Drug Discov 4 Pages: 1191-1199
  • Peer Reviewed
• [Journal Article] Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates. (2008)
  • Author(s): Tsuboi T, Takeo S, Iriko H, Jin L, Tsuchimochi M, Matsuda S, Han ET, Otsuki H, Kaneko O, Sattabongkot J, Udomsangpetch R, Sawasaki T, Torii M, Endo Y.
  • Journal Title: Infect Immun. 76 Pages: 1702-1708
  • Peer Reviewed
• [Journal Article] Diversity and evolution of the rhoph1/clag multigene family of Plasmodium falciparum. (2008)
  • Author(s): Iriko H, Kaneko O, Otsuki H, Tsuboi T, Su XZ, Tanabe K, Torii M.
  • Journal Title: Mol Biochem Parasitol. 158 Pages: 11-21
  • Peer Reviewed
• [Journal Article] Diversity and evolution of the rhophl/clag multigene family of Plasmodium falciparum. (2008)
  • Author(s): Iriko H
  • Journal Title: Mol. Biochem. Parasitol. 158 Pages: 11-21
  • Peer Reviewed
• [Journal Article] Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates. (2008)
  • Author(s): Tsuboi T
  • Journal Title: Infect. Immun 76 Pages: 1702-1708
  • Peer Reviewed
• [Journal Article] Disruption of the Plasmodium berghei 2-Cys peroxiredoxin TPx-1 gene hinders the sporozoite development in the vector mosquito. (2008)
  • Author(s): Yano K
  • Journal Title: Mol. Biochem. Parasitol. 159 Pages: 142-145
  • Peer Reviewed
• [Journal Article] Detection of four Plasmodium species by genus-and species-specific loop-mediated isothermal amplification for clinical diagnosis. (2007)
  • Author(s): Han ET, Watanabe R, Sattabongkot J, Khuntirat B, Sirichaisinthop J, Iriko H, Jin L, Takeo S, Tsuboi T.
  • Journal Title: J Clin Microbiol. 45 Pages: 2521-2528
  • Peer Reviewed
• [Journal Article] Cell-free production of functional Plasmodium falciparum dihydrofolate reductase-thymidylate synthase. (2007)
  • Author(s): Mudeppa DG, Pang CKT, Tsuboi T, Endo Y, Buckner FS, Varani G, Rathod PK.
  • Journal Title: Mol Biochem Parasitol. 151 Pages: 216-219
  • Peer Reviewed
• [Journal Article] The Plasmodium falciparum RhopH2 promoter and first 24 amino acids are sufficient to target proteins to the rhoptries. (2007)
  • Author(s): Ghoneim A, Kaneko O, Tsuboi T, Torii M.
  • Journal Title: Parasitol Int. 56 Pages: 31-43
  • NAID: 10018566061
  • Peer Reviewed
• [Journal Article] Cell-free production of functional Plasmodium dihydrofolate reductase-thymidylate synthase (2007)
  • Author(s): Mudeppa DG
  • Journal Title: Mol. Biochem. Parasitol. 151 Pages: 216-219
  • Peer Reviewed
• [Journal Article] The Plasmodium falciparum RhopH2 promoter and first 24 amino acids are sufficient to target proteins to the rhoptries (2007)
  • Author(s): Ghoneim A
  • Journal Title: Parasitol. Int 56 Pages: 31-43
  • Peer Reviewed
• [Journal Article] Detection of four Plasmodium species by genus-and species-specific loop-mediated isothermal amplification for clinical diagnosis (2007)
  • Author(s): Han ET
  • Journal Title: J. Clin. Microbiol 45 Pages: 2521-2528
  • Peer Reviewed
• [Presentation] Wheat germ cell-free protein synthesis system : a breakthrough for the post-genome malaria vaccine candidate discovery (2009)
  • Author(s): Tsuboi T
  • Organizer: Vivax malaria research III : 2009 and beyond
  • Place of Presentation: Gamboa, Panama
  • Year and Date: 2009-05-25
• [Presentation] Identificationof novel blood-stage vaccine candidates against Plasmodium falciparum by high-throughput immunoscreening. (2009)
  • Author(s): Takeo S.
  • Organizer: ASTMH 58th annual meeting
  • Place of Presentation: Washington DC, USA
• [Presentation] Immunization with N-terminal region of a gametocyte protein Pfs230 successfully induce transmission-blocking antibodies against Plasmodium falciparum. (2009)
  • Author(s): Tachibana M.
  • Organizer: ASTMH 58th annual meeting
  • Place of Presentation: Washington DC, USA
• [Presentation] Discovering novel pre-erythrocytic antigens for malaria vaccines. (2009)
  • Author(s): Aguiar JC.
  • Organizer: ASTMH 58th annual meeting
  • Place of Presentation: Washington DC, USA
• [Presentation] Immunization with N-terminal region of a gametocyte protein Pfs230 successfully induce transmission-blocking antibodies against Plasmodium falciparum. (2009)
  • Author(s): Tachibana M.
  • Organizer: The 9th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji, Japan.
• [Presentation] Wheat germ cell-free protein synthesis system: a breakthrough for the post-genome malaria vaccine candidate discovery. (2009)
  • Author(s): Tsuboi T.
  • Organizer: Vivax malaria research III: 2009 and beyond, Gamboa, Panama
• [Presentation] fs230: Prefertilization Transmission-blocking Vaccine Candidate. (2009)
  • Author(s): Tsuboi T.
  • Organizer: Malaria Transmission Blocking Strategies. Bangkok
  • Place of Presentation: Thailand
• [Presentation] Wheat germ eel1-free system : A breakthrough in malaria vaccine candidate discovery. (2008)
  • Author(s): Tsuboi T
  • Organizer: 17th International Congress for Tropical Medicine and Malaria
  • Place of Presentation: Jeju Island, Korea
  • Year and Date: 2008-10-02
• [Presentation] Wheat germ cell-free protein synthesis system: a key tool for novel malaria vaccine candidate discovery. (2008)
  • Author(s): Tsuboi T.
  • Organizer: Symposium on Capacity Building for Malaria Vaccine Development
  • Place of Presentation: Pune, India
  • Year and Date: 2008-06-21
• [Presentation] Genome-wide malaria vaccine candidate discovery using wheat germ cell-free system. (2008)
  • Author(s): Tsuboi T.
  • Organizer: JSPS presents Sweden-Japan joint Seminar, Malaria Research - Diversity & Control
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2008-06-11
• [Presentation] Immunization with recombinant proteins of a gametocyte protein Pfs230 expressed using wheat germ cell-free system successfully induce transmission-blocking antibodies against Plasmodium falciparum. (2008)
  • Author(s): Tachibana M.
  • Organizer: ASTMH 57th annual meeting
  • Place of Presentation: New Orleans, USA
• [Presentation] Novel antigens at Plasmodium falciparum schizont-merozoite stages as potential vaccine candidates. (2008)
  • Author(s): Takeo S.
  • Organizer: ASTMH 57th annual meeting
  • Place of Presentation: New Orleans, USA
• [Presentation] A complex formation of rhoptry neck protein 2 with a microneme protein, AMA1, in Plasmodium falciparum. (2008)
  • Author(s): Cao J.
  • Organizer: ASTMH 57th annual meeting
  • Place of Presentation: New Orleans, USA
• [Presentation] Wheat germ cell-free system: A breakthrough in malaria vaccine candidate discovery. (2008)
  • Author(s): Tsuboi T, Takeo S, Otsuki H, Torii M.
  • Organizer: 17th International Congress for Tropical Medicine and Malaria
  • Place of Presentation: Jeju Island, Korea
• [Presentation] Erythrocyte- Binding-Like molecule and Virulence of Plasmodium yoelii. (2008)
  • Author(s): Otsuki H.
  • Organizer: 19th Annual Molecular Parasitology Meeting
  • Place of Presentation: Woods Hole, USA
• [Presentation] Novel antigens at Plasmodium falciparum schizont-merozoite stages as potential vaccine candidates. (2008)
  • Author(s): Tsuboi T.
  • Organizer: The 8th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji, Japan.
• [Presentation] Expression of malaria vaccine candidates using a wheat germ cell-free protein synthesis system without codon optimization. (2008)
  • Author(s): Tsuboi T.
  • Organizer: hird molecular Approaches to Malaria Meeting
  • Place of Presentation: Lorne, Australia
• [Presentation] Functional production of malaria proteins with wheat germ cell-free system. Keystone Symposia (2008)
  • Author(s): Takeo S.
  • Organizer: Structural Genomics and its Applications to Chemistry
  • Place of Presentation: Biology and Medicine, Steamboat Springs, USA
• [Presentation] Novel sporozoite antigen discovery of Plasmodium falciparum screened using human immunesera (2007)
  • Author(s): Tsuboi T
  • Organizer: The 7th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji,Hyogo,Japan
  • Year and Date: 2007-09-05
• [Presentation] Novel sporozoite antigen discoverly of Plasmodium falciparum screened using human antisera. (2007)
  • Author(s): Tsuboi T.
  • Organizer: ASTMH 56th annual meeting
  • Place of Presentation: Philadelphia, USA
• [Presentation] Chitinase: active recombinant protein from Plasmodium vivax. (2007)
  • Author(s): Takeo S.
  • Organizer: ASTMH 56th annual meeting
  • Place of Presentation: Philadelphia, USA
• [Presentation] Plasmodium falciparum rhoptry neck protein (PfRON2) expressed at both erythrocytic and pre-erythrocytic invasive parasites. (2007)
  • Author(s): Cao J.
  • Organizer: The 7th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji, Japan.
• [Presentation] Novel sporozoite antigen discovery of Plasmodium falciparum screened using human immunesera (2007)
  • Author(s): Tsuboi T.
  • Organizer: The 7th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji, Japan.
• [Presentation] Transmission-blocking activity of DNA vaccine encoding Plasmodium vivax gametocyte protein Pvs230 (2007)
  • Author(s): Tachibana M.
  • Organizer: ASTMH 56th annual meeting
  • Place of Presentation: Philadelphia, USA
• [Remarks]
  • URL: http://www.ehime-u.ac.jp/~cellfree/proteomedical/malaria.html
• [Remarks]
  • URL: http://www.ehime-u.ac.jp/~cellfree/proteomedical/malaria.html
• [Remarks]
  • URL: http://www.ehime-u.ac.jp/~cellfree/proteomedical/malaria.html