Olfactory ensheathing cell differentiation for olfactory nervous glia, and establishment of the spinal cord injury treatment model

• Project/Area Number: 19500275
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Keio University
• Principal Investigator: MATSUZAKI Yumi (2008) Keio University, 医学部, 准教授 (50338183); 佐藤 悦子 (2007) Keio University, 医学部, 助教 (60244589)
• Co-Investigator(Kenkyū-buntansha): 松崎 有未 慶應義塾大学, 医学部, 准教授 (50338183)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 嗅粘膜 / OEC / 神経堤 / 嗅神経幹細胞

Research Abstract

Olfactory ensheathing cell (OEC)は、脊髄損傷の移植療法として注目されているが、基礎的研究は不十分である。我々はOEC の基礎的性質を明らかにするために本研究を行った。その結果、OEC の治療効果は軸策伸展能の単独効果ではないこと、EC が神経堤由来である可能性が高いこと、嗅粘膜由来sphere がOEC の供給源になりうること等を明らかにした。

Research Products

• [Journal Article] Bidirectional signaling through ephrinA2-EphA2 enhances osteoclastogenesis and suppresses osteoblastogenesis (2009)
  • Author(s): Irie N, Takada Y, Watanabe Y, Matsuzaki Y, Naruse C, Asano M, Iwakura Y, Suda T, Matsuo K
  • Journal Title: J Biol Chem
  • Peer Reviewed
• [Journal Article] Development of mesenchymal stem cells partially originate from the neural crest (2009)
  • Author(s): Morikawa S, Mabuchi Y, Niibe K, Suzuki S, Nagoshi N, Sunabori , Shimmura S, Nagai Y, Nakagawa T, Okano H, Matsuzaki Y
  • Journal Title: BBRC 379(4) Pages: 1114-1119
  • Peer Reviewed
• [Journal Article] Neovascularization promoted by mononuclear cell transplantation after transient cerebral ischemia in mice (2009)
  • Author(s): Funabiki T, Namiki J, Suzuki S, Matsuzaki Y, Aikawa N
  • Journal Title: Inflammation and Regeneration 29(1) Pages: 66-72
  • NAID: 10024797192
  • Peer Reviewed
• [Journal Article] Prospective isolation and identification of human mesenchymal stem cells by flow cytometry (2009)
  • Author(s): Mabuchi Y, Morikawa S, Suzuki S, Sunabori T, Okano H, and Matsuzaki Y
  • Journal Title: Inflammation and Regeneration 29(1) Pages: 3-78
  • NAID: 10024797216
  • Peer Reviewed
• [Journal Article] Evaluation of human fetal neural stem/progenitor cells as a source for cell replacement therapy for neurological disorders : Properties and tumorigenicity after long-term in vitro maintenance (2009)
  • Author(s): Ogawa D, Okada Y, Nakamura M, Kanemura Y, Okano HJ, Matsuzaki Y, Shimazaki T, Ito M, Ikeda E, Tamiya T, Nagao S, Okano H
  • Journal Title: J Neurosci Res. 87(2) Pages: 307-317
  • Peer Reviewed
• [Journal Article] Fbxw7 acts as acritical fail-safe against premature loss of hematopoietic stem cells and development of T-ALL (2008)
  • Author(s): Matsuoka S, Oike Y, Onoyama I, Iwama A, Arai F, Takubo K, Mashimo Y, Oguro H, Nitta E, Ito K, Miyamoto K, Yoshiwara H, Hosokawa K, Nakamura Y, Gomei Y, Iwasaki H, Hayashi Y, Matsuzaki Y, Nakayama K, Ikeda Y, Hata A, Chiba S, Nakayama KI, Suda T
  • Journal Title: Genes Dev. 22(8) Pages: 986-91
  • Peer Reviewed
• [Journal Article] Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad (2008)
  • Author(s): Nagoshi N, Shibata S, Kubota Y, Nakamura M, Nagai Y, Satoh E, Morikawa S, Okada Y, Mabuchi Y, Katoh H, Okada S, Fukuda K, Suda T, Matsuzaki Y, Toyama Y, Okano H
  • Journal Title: Cell Stem Cell 2(4) Pages: 392-403
  • Peer Reviewed
• [Journal Article] The role of graft and host accommodation in a hamster-to-rat cardiac transplantation model (2008)
  • Author(s): Komori K, Fuchimoto Y, Morikawa Y, Obara H, Kawachi S, Tanabe M, Hoshino K, Shimazu M, Matsuzaki Y, Kitajima M
  • Journal Title: Transplantation 85(1) Pages: 112-117
  • Peer Reviewed
• [Journal Article] Stage/Cell cycle phase specific nestin gene expression coordinated with the morphological alteration of embryonic cortical neural progenitor cells (2008)
  • Author(s): Sunabori T., Tokunaga A., Nagai T., Sawamoto K., Okabe M., Miyawaki A., Matsuzaki Y., Miyata T., Okano H.
  • Journal Title: J. Cell Sci 121(Pt 8) Pages: 1204-1212
  • Peer Reviewed
• [Journal Article] Isolation and characterization of dendritic cells from common marmosets for preclinical studies on cell therapy (2008)
  • Author(s): Ohta S., Ueda Y., Yaguchi M., Matsuzaki Y., Nakamura M., Toyama Y., Tanioka N., Tamaoki N., Nomura T., Okano H., Kawakami Y., Toda M.
  • Journal Title: Immunology 123(4) Pages: 566-574
  • Peer Reviewed
• [Journal Article] bxw7 acts as a critical fail-safe against premature loss of hematopoietic stem cells and development of T-ALL. (2008)
  • Author(s): Matsuoka S, et al. (24名中18番目)
  • Journal Title: Genes Dev. 22 Pages: 986-991
  • Peer Reviewed
• [Journal Article] Ontogeny and multipotency of neural crest-derived stemcells in mouse bone marrow, dorsal root ganglia, and whisker pad. (2008)
  • Author(s): Nagoshi N, et al. (16名中14番目)
  • Journal Title: Cell Stem Cell 2 Pages: 392-403
  • Peer Reviewed
• [Journal Article] The role of graft and host accommodation in a hamster-to-rat cardiac transplantation model. (2008)
  • Author(s): Komori K, et al. 10名中9番目
  • Journal Title: Transplantation 85 Pages: 112-117
  • Peer Reviewed
• [Journal Article] Stage/Cell cycle phase specific nestin gene expressioncoordinated with the morphological alteration of embryonic cortical neural progenitor cells. (2008)
  • Author(s): Sunabori T, et al. 9名中7番目
  • Journal Title: J. Cell Sci. 121 Pages: 1204-1212
  • Peer Reviewed
• [Journal Article] Isolation and characterization of dendritic cells from common marmosets for preolinical studies on cell therapy. (2008)
  • Author(s): Ohta S, et al. 12名中4番目
  • Journal Title: Immunology 123 Pages: 566-574
  • Peer Reviewed
• [Journal Article] Side population in human uterine myometrium displays phenotypic and functional characteristics of myometrial stem cells (2007)
  • Author(s): Ono M., Maruyama T., Masuda H., Kajitani T., Nagashima T., Arase Toru., Ito M., Ohta K., Uchida H., Asada H., Yoshimura Y., Okano H., Matsuzaki Y.
  • Journal Title: Proc Natl Acad Sci USA 104(47) Pages: 18700-18705
  • Peer Reviewed
• [Journal Article] Identification of a neuron-specific human gene, KIAA1110, that is a guanine nucleotide exchange factor for ARF1 (2007)
  • Author(s): Hattori Y, Ohta S, Hamada K, Yamada-Okabe H, Kanemura Y, Matsuzaki Y, Okano H, Kawakami Y, Toda M
  • Journal Title: Biochem Biophys Res Commun 364(4) Pages: 737-742
  • Peer Reviewed
• [Journal Article] Angiotensin II type 1receptor blockade prevents decrease in adult stem-like cells in kidney after ureteral obstruction (2007)
  • Author(s): Marumo T., Hishikawa K., Matsuzaki Y., Imai N., Takase O., Shimosawa T., Okano H., Fujita T.
  • Journal Title: Eur. J. Pharmacol 573(1-3) Pages: 216-220
  • Peer Reviewed
• [Journal Article] Administration of Granulocyte Colony-Stimulating Factor after Myocardial Infarction Enhances the Recruitment of Hematopoietic Stem Cell-Derived Myofibroblasts and Contributes to Cardiac Repair (2007)
  • Author(s): Fujita J, Mori M, Kawada H, Ieda Y, Tsuma M, Matsuzaki Y, Kawaguchi H, Yagi T, Yuasa S, Endo J, Hotta T, Ogawa S, Okano H, Yozu R, Ando K, Fukuda K
  • Journal Title: Stem Cells 25(11) Pages: 2750-2759
  • Peer Reviewed
• [Journal Article] Inhibition of Histone Deacetylase Activates Side Population Cells in Kidney and Partially Reverses Chronic Renal Injury (2007)
  • Author(s): Imai N, Hishikawa K, Marumo T, Hirahashi J, Inowa T, Matsuzaki Y, Okano H, Kitamura T, Salant D, Fujita T
  • Journal Title: Stem Cells 25(10) Pages: 2469-2475
  • Peer Reviewed
• [Journal Article] Two distinct stem cell lineages in murine bone marrow (2007)
  • Author(s): Koide Y, Morikawa S, Mabuchi Y, Muguruma Y, Hiratsu E, Hasegawa K, Kobayashi M, Ando K, Kinjo K, Okano H, Matsuzaki Y
  • Journal Title: Stem Cells 25(5) Pages: 1213-1221
  • Peer Reviewed
• [Presentation] Prospective identification of murine and human mesenchymal stem cell (2008)
  • Author(s): Yumi Matsuzaki
  • Organizer: 内藤記念財団シンポジウム
  • Place of Presentation: 湘南
  • Year and Date: 2008-11-13
• [Presentation] ヒト骨髄間葉系幹細胞の分離と同定 (2008)
  • Author(s): 松崎有未
  • Organizer: 第18回日本サイトメトリー学会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-28
• [Presentation] ヒト胃癌幹細胞株におけるSide population (SP)細胞 (2008)
  • Author(s): 松崎有未・福田和正
  • Organizer: 第7回日本再生医療学会、癌幹細胞シンポジウム、名古屋
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-03-13
• [Presentation] 細胞表面抗原によるヒト間葉系幹細胞の分離とその性状解析析 (2008)
  • Author(s): 松崎有未
  • Organizer: 特定領域研究~幹細胞の可塑性と未分化性維持機構~成果公開シンポジウム
  • Place of Presentation: 学術総合センター
  • Year and Date: 2008-02-27
• [Presentation] フローサイトメトリーを用いたヒト間葉系幹細胞分離技術 (2008)
  • Author(s): 松崎有未
  • Organizer: 慶應義塾先端科学技術シンポジウム
  • Place of Presentation: 東京慶應義塾大学三田キャンパス西校舎ホール
  • Year and Date: 2008-02-04
• [Presentation] Hideyuki Okano, Prospective identification of murine and human mesenchymal stem cells (2007)
  • Author(s): Yumi Matsuzaki
  • Organizer: 慶應義塾大学21世紀COEシンポジウム
  • Place of Presentation: 慶應義塾大学信濃町キャンパス
  • Year and Date: 2007-11-29
• [Presentation] ヒトMSC の新規分離方法 (2007)
  • Author(s): 松崎有未
  • Organizer: 第10回日本組織工学会シンポジウム
  • Place of Presentation: 大手町サンケイプラザ
  • Year and Date: 2007-11-09
• [Presentation] 間葉系幹細胞研究の展望 (2007)
  • Author(s): 松崎有未
  • Organizer: 第28回日本炎症再生医学会、間葉系幹細胞ワークショップ
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2007-08-02
• [Presentation] ヒト胃癌細胞株におけるSP細胞の機能 (2007)
  • Author(s): 松崎有未
  • Organizer: 第17回日本フローサイトメトリー学会学術集会、癌幹細胞シンポジウム
  • Place of Presentation: サンルートプラザ東京
  • Year and Date: 2007-07-05
• [Presentation] ソーティングの原理とSP 細胞分離 (2007)
  • Author(s): 松崎有未
  • Organizer: 第17回日本フローサイトメトリー学会学術集会
  • Place of Presentation: サンルートプラザ東京
  • Year and Date: 2007-07-05
• [Presentation] 間葉系幹細胞の分離・同定と発生系譜に関する考察 (2007)
  • Author(s): 松崎有未
  • Organizer: 第5回幹細胞シンポジウム
  • Place of Presentation: 兵庫県立淡路夢舞台国際会議場
  • Year and Date: 2007-05-18
• [Patent(Industrial Property Rights)] ヒト間葉系幹細胞濃縮方法 (2007)
  • Inventor(s): 松崎有未、馬渕洋、森川暁、岡野栄之
  • Industrial Property Rights Holder: 学校法人慶應義塾
  • Industrial Property Number: 2007-231298
  • Filing Date: 2007-09-06