| Project/Area Number |
19500295
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
|
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| Research Institution | Kawasaki Medical School (2008)
The University of Tokushima (2007) |
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Principal Investigator |
TOIDA Kazunori Kawasaki Medical School, 解剖学, 教授 (40253405)
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| Project Period (FY) |
2007 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 三次元構造 / 神経回路 / シナプス / 嗅球 / GFP / 介在ニューロン / 電子顕微鏡 / tyrosine hydroxylase (TH) / glutamic acid decarboxylase (GAD) / EPSC / green fluorescence protein (GFP) / デジタル画像解析 / 遠心性入力 |
|---|
| Research Abstract |
We have reported neuronal heterogeneity underlying olfactory information processing in the olfactory bulb glomeruli. By means of combined electrophysiology, light and electron microscopy, bulbar neurons were identified both chemically (by GFP transgenic mice) and morphologically (by Neurolucida). Patch clamp to TH-and GAD-GFP neurons exhibited two types of response to olfactory input, ON-direct and ON-indirect driven types. These finding were confirmed by electron microscopy revealing heterogeneous synaptic contact with special focus on input to then, and suggested elaborate architecture of synaptic circuit based on neuronal heterogeneity chemically, physiologically and morphologically.
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