The role of brain molecular chaperone 14-3-3 protein, which regulates the progress of HIV-1 encephalopathy and prion disease

• Project/Area Number: 19500308
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: The University of Tokushima
• Principal Investigator: YANO Mihiro The University of Tokushima, 疾患酵素学研究センター, 准教授 (40304555)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: エイズ脳症 / 14-3-3蛋白質 / ストレス蛋白質 / 神経細胞死 / HIV-1, gp120 / 分子シャペロン / ウイルス / 血液脳関門

Research Abstract

エイズ脳症発症のメカニズムを明らかにするため、ヒト脳微小血管内皮細胞(HBMEG)培養系の簡易血液脳関門(BBB)モデルを用いて、H-V-1gp120によるBBB破綻とその制御機構について解析を行った。その結果、14-3-3tが、gp120によるZO-1等のタイトジャンクション(TJ)構成蛋白質のプロテアソームによる分解を防ぐ事で、TJの崩壊に抑制的に機能している事を明らかにした。

Research Products

• [Journal Article] HIV-1 immunodeficiency virus typel gp120-mediated disruption of tight junction proteins by induction of proteasome-mediated degradation of zonula occludens-1 and -2 in human brain microvascular endothelial cells (2008)
  • Author(s): Shinichi Nakamuta, Hiroshi Endo, Youichiro Higashi, Aoi Kousaka, Hiroshi Yamada, Mihiro Yano and Hiroshi Kido
  • Journal Title: Journal of NeuroVirology 14 Pages: 186-195
  • Peer Reviewed
• [Journal Article] HIV-1 gp120-mediated disruption of tight junction proteins by induction of proteasome-mediated degradation of zonula occludens-1 and -2 in human brain microvascular endothelial cells (2008)
  • Author(s): Shinichi Nakamuta
  • Journal Title: Journal of Neurovirology 14, 3 Pages: 186-195
  • Peer Reviewed
• [Journal Article] HIV-1 gp120-mediated disruption of tight junction proteins by induction of proteasome-mediated degradation of zonula occludens-1 and-2 in human brainmicrovascular endothelial cells (2008)
  • Author(s): Shinichi Nakamuta
  • Journal Title: Journal of Neurovirology
  • Peer Reviewed
• [Journal Article] Gatekeeper role of 14-3-3τ protein in HIV-1 gp120-mediated apoptosis of human endothelial cells by inactivation of Bad (2007)
  • Author(s): Mihiro Yano
  • Journal Title: AIDS 21 Pages: 911-920
  • Peer Reviewed
• [Presentation] Ibuprofen Increases Cisplatin-induced Apoptosis in Lung Adenocarcinoma By Suooressin2 Hsp70 (2008)
  • Author(s): 矢野仁康、遠藤弘史、木戸博
  • Organizer: 31回日本分子生物学会年会、第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] The Biological Functions of 14-3-3 as a Stress Protein (2008)
  • Author(s): Mihiro Yano
  • Organizer: Gordon Research Conferences, The Biology of 14-3-3 Proteins
  • Place of Presentation: Ventura,USA
  • Year and Date: 2008-02-27
• [Presentation] The biological functions of 14-3-3 as a stress protein (2008)
  • Author(s): Mihiro Yano
  • Organizer: Gordon Research Conferences
  • Place of Presentation: 米国, Ventura
  • Year and Date: 2008-02-26
• [Presentation] Diclofenac exerts anti-tumor activity via 14-3-3 proteins-dependent activation of mitochondrial apoptosis pathway (2008)
  • Author(s): Hiroshi Endo, Mihiro Yano, Hiroshi Kido
  • Organizer: Gordon Research Conferences
  • Place of Presentation: 米国, Ventura
  • Year and Date: 2008-02-25
• [Presentation] Diclofenac exerts anti-tumor activity via 14-3-3 proteins-dependent activation of mitochondrial apoptosis pathway (2007)
  • Author(s): 遠藤弘史、矢野仁康、木戸博
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Book] The Molecular Chaperones in the Ubigitin-Proteasome System. The Ubiquitin Proteasome System in the Central Nervous System, From Physiology to Pathology. Mario DiNapoli, Cezary Wojcik Nova BiomedicaI Books (2007)
  • Author(s): Mihiro Yano et.al.
  • Publisher: Nova Science Publishers