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The role of PPAR in metabolic syndrome with novel genetically modified rabbits

Research Project

Project/Area Number 19500362
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionSaga University

Principal Investigator

KITAJIMA Shuji  Saga University, 総合分析実験センター, 准教授 (70284643)

Co-Investigator(Kenkyū-buntansha) FAN Jianglin  山梨大学, 医学工学総合研究部, 教授 (60272192)
NISHIJIMA Kazutoshi  佐賀大学, 総合分析実験センター, 助教 (70435874)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords遺伝子組換え / ウサギ / 肥満 / 病態モデル / PPAR
Research Abstract

We generated transgenic (Tg) rabbits as a novel human disease model for metabolic syndrome. PPAR-gamma has been believed to be associated with pathologic status of it. Tg rabbits was designed to specifically overexpress human PPAR-gamma in their adipose tissue. As the results, we confirmed specific expression of mRNA of transgene in the adipose tissue of Tg rabbits, however, we could not find phenotypic differences (pathologic status) between Tg rabbits and wild type one. It is thought that more detailed further examination such as manifestation protein activity is required in future.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (43 results)

All 2010 2009 2008 2007 Other

All Journal Article (19 results) (of which Peer Reviewed: 7 results) Presentation (21 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Age is an important factor influence on the number of recovered eggs in superovulated rabbits : Analysis of our past 5 years data of 509 rabbits superovulated by FSH or PMSG.2010

    • Author(s)
      Kitajima, S.
    • Journal Title

      Scand.J.Lab.Anim.Sci. (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ウサギ精子・胚の凍結保存とバイオリソース2010

    • Author(s)
      北嶋修司
    • Journal Title

      アニテックス (印刷中)

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Human C-reactive protein does not promote atherosclerosis in transgenic rabbits.2009

    • Author(s)
      Koike, T., Kitajima, S., Yu, Y., Nishijima, K., Zhang, J., Ozaki, Y., Morimoto, M., Watanabe, T., Bhakdi, S., Asada, Y., Chen, Y.E., Fan, J.
    • Journal Title

      Circulation 120

      Pages: 2088-2094

    • Related Report
      2009 Final Research Report
  • [Journal Article] Expression of human apoAII in transgenic rabbits leads to dyslipidemia: a new model for combined hyperlipidemia.2009

    • Author(s)
      Koike, T., Kitajima, S., Yu, Y., Li, Y., Nishijima, K., Liu, E., Sun, H., Ahmed Bilal Waqar, A.B., Shibata, N., Inoue, T., Wang, Y., Zhang, B, Kobayashi, J, Morimoto, M., Saku, K., Watanabe, T., Fan, J.
    • Journal Title

      Arterioscler. Thromb. Vasc. Biol. 29

      Pages: 2047-2053

    • Related Report
      2009 Final Research Report
  • [Journal Article] Expression of human apoAII in transgenic rabbits leads to dyslipidemia : a new model for combined hyperlipidemia.2009

    • Author(s)
      Koike, T., Kitajima, S.(equally contributed)
    • Journal Title

      Arterioscler. Thromb. Vasc.Biol. 29

      Pages: 2047-2053

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human C-reactive protein does not promote atherosclerosis in transgenic rabbits.2009

    • Author(s)
      Koike, T., Kitajima, S.(equally contributed)
    • Journal Title

      Circulation 120

      Pages: 2088-2094

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      Kitajima, S.
    • Journal Title

      Rabbit Biotechnology : rabbit genomics, transgenesis, cloning and models.(Springer Heidelberg)

      Pages: 3-12

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Temporal and quantitative analysis of expression of metalloproteinase (MMPs) and their endogenous inhibitors in atherosclerotic lesions.2008

    • Author(s)
      Yu, Y., Koike, T., Kitajima, S., Liu, E., Morimoto, M., Shiomi, M., Hatakeyama, K., Asada, Y., Wang, K-Y., Sasaguri, Y., Watanabe, T., Fan, J.
    • Journal Title

      Histol. Histopathol. 23

      Pages: 1503-1516

    • Related Report
      2009 Final Research Report
  • [Journal Article] Matrix metalloproteinase 12 is a factor that accelerates the initiation of atherosclerosis and stimulates the progression of fatty streaks to fibrous plaques in transgenic rabbits.2008

    • Author(s)
      Yamada, S., Wang, K-Y., Tanimoto, A., Fan, J., Shimajiri, S., Kitajima, S., Morimoto, M., Tsutsui, M., Watanabe, T., Yasumoto, K., Sasaguri, Y.
    • Journal Title

      Am. J. Pathol. 172

      Pages: 1419-1429

    • Related Report
      2009 Final Research Report
  • [Journal Article] 凍結精子による遺伝子組換えウサギの系統保存2008

    • Author(s)
      北嶋修司, 森本正敏
    • Journal Title

      関西実験動物研究会会報 30

      Pages: 16-20

    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Journal Article] Increased expression of vascular endothelial growth factor in kidney leads to progressive impairment of glomerular functions.2007

    • Author(s)
      Liu, E., Morimoto, M., Kitajima, S., Yu, Y., Koike, T., Shiiki, H., Nagata, M., Watanabe, T., Fan, J.
    • Journal Title

      J. Am. Soc. Nephrol. 18

      Pages: 2094-2104

    • Related Report
      2009 Final Research Report
  • [Journal Article] Lp(a) enhances coronary atherosclerosis in transgenic Watanabe heritable hyperlipidemic rabbits.2007

    • Author(s)
      Kitajima, S., Jin, Y., Koike, T., Yu, Y., Liu, E., Morimoto, M., Shiomi, M., Marcovina, S., Watanabe, N. Fan, J.
    • Journal Title

      Atherosclerosis 193

      Pages: 269-276

    • Related Report
      2009 Final Research Report
  • [Journal Article] Numbers of publications related to laboratory animals.2007

    • Author(s)
      Zhao, S., Liu, E., Chu, Y., Zheng, H., Kitajima, S., Morimoto, M.
    • Journal Title

      Scand. J. Lab. Anim. Sci. 34

      Pages: 81-86

    • Related Report
      2009 Final Research Report
  • [Journal Article] Re-establishment of Complement C6-deficeint Rabbit Colony by Cryopreserved Sperm Transported from Abroad.2007

    • Author(s)
      Liu, E., Kitajima, S., Wiese, E., Reifenberg, K., Morimoto, M., Watanabe, T., Fan, J.
    • Journal Title

      Exp. Anim. 55(2)

      Pages: 167-171

    • Related Report
      2009 Final Research Report
  • [Journal Article] ヒト疾患モデルとしての遺伝子組換えウサギの開発とその系統保存.2007

    • Author(s)
      北嶋修司, 詫广茂信, 友田清光, 森本正敏
    • Journal Title

      九州実験動物雑誌 23

      Pages: 31-45

    • Related Report
      2009 Final Research Report
  • [Journal Article] Re-establishment of Complement C6-deficeint Rabbit Colony by Cryopreserved Sperm Transported from Abroad2007

    • Author(s)
      Liu, E., Kitajima, S., Wiese, E., Reifenberg, K., Morimoto, M., Watanabe.T.and Fan.J.
    • Journal Title

      Exp.Anim. 55

      Pages: 167-171

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lp(a) enhances coronary atherosclerosis in transgenic Watanabe heritable hyperlipidemic rabbits2007

    • Author(s)
      Kitajima, S., Jin, Y., Koike, T., Yu, Y., Liu, E., Morimoto, M., Shiomi, M., Marcovina, S., Watanabe, N.and Fan.J.
    • Journal Title

      Atherosclerosis 193

      Pages: 269-276

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased expression of vascular endothelial growth factor in kidney leads to progressive impairment of glonlerular functions2007

    • Author(s)
      Liu, E., Morimoto, M., Kitajima, S., Yu, Y., Koike, T., Shiiki, H., Nagata, M., Watanabe.T., Fan.J.
    • Journal Title

      J.Am.Soc.Nephrol. 18

      Pages: 2094-2104

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ヒト疾患モデルとしての遺伝子組換えウサギの開発とその系統保存2007

    • Author(s)
      北嶋 修司, 詫广 茂信, 友田 清光, 森本 正敏
    • Journal Title

      九州実験動物雑誌 23

      Pages: 31-45

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] ウサギの血清分離方法の検討2009

    • Author(s)
      漢那宗士
    • Organizer
      第57回日本実験動物学会総会
    • Place of Presentation
      新潟
    • Year and Date
      2009-10-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Technology suitable for rabbit semen conservation2009

    • Author(s)
      Kitajima, S.
    • Organizer
      3rd International Meeting on Rabbit Biotechnology
    • Place of Presentation
      西安(中国)
    • Year and Date
      2009-06-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] ウサギ採卵成績に影響を及ぼす要因の検討 : 過去5年間の採卵成績の解析2009

    • Author(s)
      北嶋修司
    • Organizer
      第56回日本実験動物学会総会
    • Place of Presentation
      埼玉
    • Year and Date
      2009-05-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] Transgenic Rabbits Expressing human C-reactive Protein2009

    • Author(s)
      Koike, T.
    • Organizer
      Arteriosclerosis, Thrombosis and Vascular Biology Annual Conference
    • Place of Presentation
      Washington DC(USA)
    • Year and Date
      2009-04-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] ウサギの血清分離方法の検討.2009

    • Author(s)
      漢那宗士, 西島和俊, 常吉梨沙, 森本正敏, 北嶋修司
    • Organizer
      第43回日本実験動物技術者協会総会
    • Place of Presentation
      新潟
    • Related Report
      2009 Final Research Report
  • [Presentation] Transgenic rabbits expressing Human C-reactive protein.2009

    • Author(s)
      Koike, T., Kitajima, S., Yu, Y., Nishijima, K., Zhang, J., Ozaki, Y., Morimoto, M., Watanabe, T., Asada, Y., Chen, E., Fan, J.
    • Organizer
      第41回日本動脈硬化学会総会・学術集会
    • Place of Presentation
      下関
    • Related Report
      2009 Final Research Report
  • [Presentation] Immunoreactivity of neuropeptides in hypothalamus of the transgenic rabbits using immunohisotchemical staining.2009

    • Author(s)
      Morimoto, M., Kitajima, S., Nishijima, K., Koike, T., Fan, J.
    • Organizer
      3rd International Meeting on Rabbit Biotechnology
    • Place of Presentation
      Xian, China
    • Related Report
      2009 Final Research Report
  • [Presentation] Comparison of two rabbit semen cryopreservation protocol-a pilot study.2009

    • Author(s)
      Szikra, D., Nagy, S., Bernder, B., Hiripi, L., Kitajima, S., Pribenszky, C., Bosze, Z.
    • Organizer
      3rd International Meeting on Rabbit Biotechnology
    • Place of Presentation
      Xian, China
    • Related Report
      2009 Final Research Report
  • [Presentation] Transgenic rabbits expressing human C-reactive protein.2009

    • Author(s)
      Koike, T., Yu, Y., Kitajima, S., Zhang, J., Bhakdi, S., Chen, E.Y., Fan, J.
    • Organizer
      3rd International Meeting on Rabbit Biotechnology
    • Place of Presentation
      Xian, China
    • Related Report
      2009 Final Research Report
  • [Presentation] Technology suitable for rabbit semen conservation.2009

    • Author(s)
      Kitajima, S., Maeda, T., Liu, E., Nishijima, K., Morimoto, M., Watanabe, T.
    • Organizer
      3rd International Meeting on Rabbit Biotechnology
    • Place of Presentation
      Xian, China
    • Related Report
      2009 Final Research Report
  • [Presentation] ウサギ採卵成績に影響を及ぼす要因の検討:過去5年間の採卵成績の解析.2009

    • Author(s)
      北嶋修司, 西島和俊, 森本正敏, 渡辺照男, 範江林
    • Organizer
      第56回日本実験動物学会総会
    • Place of Presentation
      埼玉
    • Related Report
      2009 Final Research Report
  • [Presentation] Transgenic Rabbits Expressing human C-reactive Protein.Arteriosclerosis2009

    • Author(s)
      Koike, T., Fan, J., Yu, Y., Kitajima, S., Zhang., J., Bhakdi., S., Chen, E.Y.
    • Organizer
      Thrombosis and Vascular Biology Annual Conference 2009
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] 細胞外基質分解酵素MMP-12は、初期動脈硬化巣の形成から進展に重要な促進因子である.2008

    • Author(s)
      山田壮亮, 王克〓, 谷本昭英, 範江林, 島尻正平, 北嶋修司, 森本正敏, 渡邊照男, 笹栗靖之
    • Organizer
      第3回フォーラム「医療に貢献する実験用ウサギの新しい展開」
    • Place of Presentation
      神戸
    • Year and Date
      2008-07-26
    • Related Report
      2009 Final Research Report
  • [Presentation] 凍結融解後のウサギ精子受精能に及ぼす冷却速度の影響について2007

    • Author(s)
      前田達弘, 劉恩岐, 詫广茂信, 北嶋修司, 森本正敏
    • Organizer
      第25回九州実験動物研究会
    • Place of Presentation
      熊本
    • Year and Date
      2007-11-10
    • Related Report
      2009 Final Research Report
  • [Presentation] 凍結融解後のウサギ精子受精能に及ぼす冷却速度の影響について2007

    • Author(s)
      前田 達弘, 劉 恩岐, 詫广 茂信, 北嶋 修司, 森本 正敏
    • Organizer
      第25回 九州実験動物研究会
    • Place of Presentation
      熊本
    • Year and Date
      2007-11-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] 凍結精子による遺伝子組換えウサギの系統保存(招待講演).「トランスレーショナルリサーチにおけるウサギの重要性」2007

    • Author(s)
      北嶋修司
    • Organizer
      第95回関西実験動物研究会
    • Place of Presentation
      神戸大学
    • Year and Date
      2007-09-14
    • Related Report
      2009 Final Research Report
  • [Presentation] 凍結精子による遺伝子組換えウサギの系統保存2007

    • Author(s)
      北嶋 修司
    • Organizer
      第95回 関西実験動物研究会
    • Place of Presentation
      神戸
    • Year and Date
      2007-09-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] 動脈硬化病変のC反応性蛋白-その由来と病態生理学的重要性.2007

    • Author(s)
      小池智也, 北嶋修司, 森本正敏, 塩見雅志, 渡辺照男, 浅田祐士郎, 畠山金太, 範江林
    • Organizer
      第24回日本疾患モデル動物学会総会
    • Place of Presentation
      筑波
    • Related Report
      2009 Final Research Report
  • [Presentation] Rabbit semen conservation.2007

    • Author(s)
      Kitajima, S.
    • Organizer
      2nd International Meeting on Rabbit Biotechnology
    • Place of Presentation
      Paris, France
    • Related Report
      2009 Final Research Report
  • [Presentation] Rabbit semen conservation2007

    • Author(s)
      Kitajima, S.
    • Organizer
      2nd International Meeting on Rabbit Biotechnology
    • Place of Presentation
      France
    • Related Report
      2007 Annual Research Report
  • [Presentation] 動脈硬化病変のC反応性蛋白-その由来と病態生理学的重要性2007

    • Author(s)
      小池 智也, 北嶋 修司, 森本 正敏, 塩見 雅志, 渡辺 照男, 浅田 祐士郎, 畠山 金太, 範 江林
    • Organizer
      第24回 日本疾患モデル動物学会総会
    • Place of Presentation
      筑波
    • Related Report
      2007 Annual Research Report
  • [Book] Improvement of rabbit production. Rabbit Biotechnology: rabbit genomics, transgenesis, cloning and models. Houdebine, L.-M. and Fan, J.2009

    • Author(s)
      Kitajima, S.
    • Publisher
      Springer
    • Related Report
      2009 Final Research Report
  • [Book] Rabbit transgenesis. Rabbit Biotechnology: rabbit genomics, transgenesis, cloning and models. Houdebine, L.-M. and Fan, J.2009

    • Author(s)
      Kitajima, S., Liu, E., Fan, J.
    • Publisher
      Springer Heidelberg
    • Related Report
      2009 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.animal.med.saga-u.ac.jp/

    • Related Report
      2009 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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