| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Severe burn scar in children results in the undesirable functional disturbance. Giant nevus in children results in the mental disturbance. This study was aimed to develop the autologous cultured dermal substitute (auto-CDS) for the treatment of these skin diseases. The results in this study proved the following evidences. 1) The cryopreservation for auto-CDS was investigated in detail. The suitable matrix for auto-CDS that is able to keep the physical properties was developed. 2) The cryopreserved and thaued CDS is able to maintain the potency to produce various growth factors including VEGF, HGF, and bFGF that are essential for angiogenesis. 3) As well as developing auto-CDS, the proper covering material for aluto-CDS was developed. This covering material is composed of a hyaluronic acid spongy sheet containing EGF. The results in culture system revealed that EGF released from covering material functions to stimulate fibroblasts in auto-CDS, and thereby fibroblasts can release more amounts of VEGF and HGF. The use of auto-CDS in conjunction with the EGF-incorporated covering material is able to provide a successful treatment for severe burn scar and giant nevus in children.
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