Physiological role of dietary fiber in aging rat

• Project/Area Number: 19500679
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Tokyo Gakugei University
• Principal Investigator: MINAMI Michiko Tokyo Gakugei University, 教育学部, 教授 (70272432)
• Co-Investigator(Kenkyū-buntansha): 中井 雄治 東京大学, 大学院・農学生命科学研究科, 特任准教授 (10321788)
• Co-Investigator(Renkei-kenkyūsha): NAKAI Yuji 東京大学, 大学院・農学生命研究科, 特任准教授 (10321788)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 食物繊維 / DNAマイクロアレイ / 遺伝子発現 / 老年期 / 遺伝子 / 栄養学 / マイクロアレイ / 発現変化 / 栄誉学

Research Abstract

To determine the physiological role of dietary fiber, 10% and 2% cellulose diet were served 40 weeks male Wister rat for a week. The composition of 12 fecal microflora was analysed by T-RFLP profile derived from the digestion of the fecal 16S rRNA gene by HhaI digestion. The fecal microflora was divided into 2 clusters. Serum PAI-1 is significantly different from 10% and 2% cellulose diet group. The effect of dietary fiber on the gene expression profiles in liver by using DNA microarray analysis.
A gene ontology analysis revealed that lipid metabolic-related genes had been markedly up-regulated.

Research Products

• [Journal Article] The Hsp90 inhibitor geldanamycin abrogates colocalization of eIF4E with eIF4E-Trnsporter into Stress Granules and Association of eIF4E wit eIF4G J.B.C. (2009)
  • Author(s): Suzuki Y., Minami, M., Suzuki M., Abe K., Zenno S., Tujimoto M., Matsumoto, K. Minami, Y.
  • Journal Title: 284(51) Pages: 35597-35604
  • Peer Reviewed
• [Journal Article] The Hsp90 Inhibitor Geldanamycin Abrogates Colocalization of eIF4E-Transport into Stress Granules and Association of eIF4E with eIF4G (2009)
  • Author(s): Yukari Suzuki, Michiko Minami, Yasufumi, Minami
  • Journal Title: Journal of Biological Chemistry 284 Pages: 35597-35604
  • Peer Reviewed
• [Journal Article] Applocation of Aspergillus saitoi Protease Preparation to Soybean Curd to Modify Its Functional and Rheological Properties Biosci. (2008)
  • Author(s): Mizuho Nishida, Tomiko Asakura, Tomomi Watanabe, Etsuko Kunizaki, Mami Matsumoto, Wakako Eto, Tomoko Tamura, Michik Minami, Akio Obata, Keiko Abe, Junko Funaki
  • Journal Title: BIotechnol. Biochem 72(2) Pages: 581-590
  • Peer Reviewed
• [Journal Article] Up-regulation of Genes Related to the Ubiquitin-Proteasome System in the Brown Adipose Tissue of 24h-Fasted Rats Biosci. (2008)
  • Author(s): Nakai. Y., Hashida. H., Kadota K., Minami. M., Shimizu. K., Matsumoto I., Katoh. H. Abe K.
  • Journal Title: Biotechnol. Biochem 72 Pages: 139-148
  • Peer Reviewed
• [Journal Article] Up-regulation of Genes Related to the Ubiquitin-Proteasome System in the Brown Adipose Tissue of 24h-Fasted Rats (2008)
  • Author(s): Nkai Y., Hashida H., Kadota K., Minami M., Shimizu K., Matsumoto L, Kato H., Abe K.
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 139-148
  • NAID: 10027523175
  • Peer Reviewed
• [Journal Article] Applicatiom of aspergillus saitoi Protease preparation of Soybean Curd of Modify Its Functional and Rheological Properties (2008)
  • Author(s): Nishinoaki M., Asakura T., Kunizaki., Matsumoto M., Eto W., Tamura T., Minami M., Obata A., Abe K and Funaki J
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 587-590
  • Peer Reviewed
• [Presentation] A Possible Role of Hsp90 in Translational Control (2010)
  • Author(s): Ysufumi Minami, Michiko Minami
  • Organizer: The complex life of RNA: From synthesis to decay
  • Place of Presentation: European molecular BiologyLaboratory(Heiderberg)
  • Year and Date: 2010-03-20
• [Presentation] A Possible Role of Hsp90 in Translational Control (2010)
  • Author(s): 南道子, 南康文
  • Organizer: The complex life of RNA : From synthesis to decay
  • Place of Presentation: EMBL Heiderberg(Germany)
  • Year and Date: 2010-03-20
• [Presentation] The Hsp90 inhibitor geldanamycin affects the formation of processing bodies and stress granules (2009)
  • Author(s): 松本健、南道子、南康文
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜国際会議場
  • Year and Date: 2009-12-10
• [Presentation] P body及びストレス顆粒に関わるHsp90の機能 (2009)
  • Author(s): 南道子、松本健、南康文
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-23
• [Presentation] 食餌性亜鉛の欠乏がラット小腸の遺伝子発現に与える影響 (2009)
  • Author(s): 中井雄治
  • Organizer: 日本農芸化学会21年度大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-03-28
• [Presentation] 食餌性亜鉛の欠乏がラット肝臓の遺伝子発現に与える影響 (2008)
  • Author(s): 成川正隆 中井雄治 南道子、三坂巧、阿部啓子
  • Organizer: 平成20年度日本農芸化学会大会
  • Place of Presentation: 名城大学
  • Year and Date: 2008-03-27