Molecular analysis on biogenesis of iron-sulfur centers of photosystem I in oxygen-evolving organisms

• Project/Area Number: 19570039
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理・分子
• Research Institution: Osaka University
• Principal Investigator: NAKAI Masato Osaka University, 蛋白質研究所, 准教授 (90222158)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 鉄硫黄クラスター / 膜蛋白質複合体 / 生合成 / 葉緑体 / 光化学系 / オルガネラ / コファクター / シロイヌナズナ / 足場蛋白質 / グルタレドキシン / 光合成

Research Abstract

鉄硫黄蛋白質はポリペプチド鎖に無機の鉄と硫黄が鉄硫黄クラスターと呼ばれる特徴的な配位構造を形成して組み込まれている生物界に広く分布する蛋白質の総称で、呼吸鎖や光化学系の電子伝達鎖におけるキーコンポーネントとして細胞内で様々な代謝活動に関与している。本研究では、酸素発生型の光合成を営む植物葉緑体やらん藻を材料に、鉄硫黄クラスター生合成の分子メカニズムに焦点を当て研究を進めた。その結果、光化学系Iの鉄硫黄クラスター活性中心の生合成に関与する因子のキャラクタリゼーションに成功した

Research Products

• [Journal Article] Arabidopsis cytosolic Nbp35 homodimer can assemble both [2Fe-2S] and [4Fe-4S] clusters in two distinct domains (2009)
  • Author(s): Kohbushi Hirokazu, Nakai Yumi, Kikuchi Shingo, Yabe Toshiki, Hori Hiroshi, Nakai Masato
  • Journal Title: Biochemical and Biophysical Research Communications 378(4) Pages: 810-815
  • Peer Reviewed
• [Journal Article] Arabidopsis cytosolic Nbp35 homodimer can assemble both [2Fe-2S] and [4Fe-4S] clusters in two distinct domains. (2009)
  • Author(s): Hirokazu Kohbushi
  • Journal Title: Biochemical and Biophysical Research Communications. 378 Pages: 810-815
  • Peer Reviewed
• [Journal Article] Non-identical contributions of two membrane-bound cpSRP components, cpFtsY and Alb3, to thylakoid biogenesis (2008)
  • Author(s): Asakura Yukari, Kikuchi Sshingo, Nakai Masato
  • Journal Title: The Plant Journal 56 Pages: 1007-1017
  • Peer Reviewed
• [Journal Article] Chloroplast outer envelope protein CHUP1 is essential for chloroplast anchorage to the plasma membrane and chloroplast movement (2008)
  • Author(s): Oikawa Kazusato, Yamasato Akihiro, Kong Sam Geun, Kasahara Masahiro, Nakai Masato, Takahashi Fumio, Ogura Yasunobu, Oikawa Kazusato, Yamasato Akihiro, Kong Sam Geun, Kasahara Masahiro, Nakai Masato, Takahashi Fumio, Ogura Yasunobu, Kagawa Takatoshi, Wada Masamitsu
  • Journal Title: Plant Physiology 148(2) Pages: 829-842
  • Peer Reviewed
• [Journal Article] Thio modification of yeast cytosolic tRNA requires a ubiquitin-related system that resembles bacterial sulfur transfer systems (2008)
  • Author(s): Nakai Yumi, Nakai Masato, Hayashi Hideyuki
  • Journal Title: Journal Biological Chemistry 283(41) Pages: 27469-27476
  • Peer Reviewed
• [Journal Article] Structural analysis of Arabidopsis CnfU protein : an iron-sulfur cluster biosynthetic scaffold in chloroplasts (2008)
  • Author(s): Yabe Toshiki, Yamashita Eiki, Kikuchi Akihiro, Morimoto Kozo, Nakagawa Atsushi, Tsukihara Tomitake, Nakai Masato
  • Journal Title: Journal of Molecular Biology 381(1) Pages: 160-173
  • Peer Reviewed
• [Journal Article] Thio modification of yeast cytosolic tRNA requires a ubiquitin-related system that resembles bacterial sulfur transfer systems. (2008)
  • Author(s): Yumi Nakai
  • Journal Title: Journal of Biological Chemistry 283 Pages: 27469-27476
  • Peer Reviewed
• [Journal Article] Structural analysis of Arabidopsis CnfU protein : an iron-sulfur cluster biosynthetic scaffold in chloroplasts. (2008)
  • Author(s): Toshiki Yabe
  • Journal Title: Journal of Molecular Biology 381 Pages: 160-173
  • Peer Reviewed
• [Journal Article] Thio modification of yeast cytosolic tRNA is an iron-sulfur protein-dependent pathway (2007)
  • Author(s): Nakai Yumi, Nakai Masato, Lill Roland, Suzuki Tsutomu, Hayashi Hideyuki
  • Journal Title: Molecular and Cellular Biology 27(8) Pages: 2841-2847
  • Peer Reviewed
• [Journal Article] Thio modification of yeast cytosolic tRNA is an iron-sulfur protein-dependent pathway. (2007)
  • Author(s): Yumi Nakai
  • Journal Title: Molecular and Cellular Biology 27 Pages: 2841-2847
  • Peer Reviewed
• [Presentation] 日本生化学会・日本分子生物学会合同大会 (2008)
  • Author(s): 中井由実, 中井正人, 林秀行
  • Organizer: ユビキチン様タンパク質Urm1と硫黄運搬タンパク質Uba4は酵母サイトゾルtRNAの硫黄修飾に必要である
  • Place of Presentation: 横浜
  • Year and Date: 2008-12-11
• [Presentation] Protein import into chloroplasts is mediated by a 1-MD translocation complex at the inner envelope membrane. (2008)
  • Author(s): Masato Nakai
  • Organizer: The EMBO Conference series “Control, co-ordination and regulation of protein targeting and translocation"
  • Place of Presentation: フランス セントマキシム
  • Year and Date: 2008-10-27
• [Presentation] 日本生化学会・日本分子生物学会合同大会 (2007)
  • Author(s): 桂真一郎, 矢部俊樹, 中井正人
  • Organizer: 葉緑体におけるcpSufEとAtGrx3の相互作用とFe-Sクラスター生合成との関連について
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] 日本生化学会・日本分子生物学会合同大会 (2007)
  • Author(s): 矢部俊樹, 堀洋, 中井正人
  • Organizer: [4Fe-4S]型鉄硫黄クラスター形成に関与する蛋白質HCF101の機能解析
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] 日本生化学会・日本分子生物学会合同大会 (2007)
  • Author(s): 中井由実, 中井正人, 林秀行
  • Organizer: 酵母tRNAの2チオ修飾と鉄硫黄クラスター形成経路の関係
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] 葉緑体におけるcpSufEとAtGrx3の相互作用とFe-Sクラスター生合成との関連について (2007)
  • Author(s): 桂 真一郎
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2007-12-11
• [Presentation] [4Fe-4S]型鉄硫黄クラスター形成に関与する蛋白質HCF101の機能解析 (2007)
  • Author(s): 矢部 俊樹
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2007-12-11
• [Presentation] CnfU, Nfu, & NifU : Similar but Distinct Fe-S Cluster Biosynthetic Scaffolds Required under Different Intracellular Conditions (2007)
  • Author(s): Nakai Masato
  • Organizer: Biogenesis of Iron Sulfur proteins : Cluster Assembly and regulation
  • Place of Presentation: フランス ヴィラドゥランス
  • Year and Date: 2007-07-10
• [Presentation] CnfU, Nfu, & NifU: Similar but Distinct Fe-S Cluster Biosynthetic Scaffolds Required under Different Intracellular Conditions. (2007)
  • Author(s): 中井 正人
  • Organizer: International Conference on Biogenesis of Iron Sulfur proteins: Cluster Assembly and Regulation
  • Place of Presentation: ヴィラデランス(フランス)
  • Year and Date: 2007-07-10
• [Presentation] 東北大学 第34回 生体分子科学討論会 (2007)
  • Author(s): 矢部俊樹, 山下栄樹, 菊池晶裕, 齋藤正男, 中井正人
  • Organizer: 葉緑体における鉄硫黄クラスター生合成機構の構造生物学
  • Place of Presentation: 仙台
  • Year and Date: 2007-06-23
• [Book] キーワード : 蛋白質の一生 (2008)
  • Author(s): 中井 正人
  • Publisher: 共立出版