Elucidation of the molecular evolution of the major histocompatibility complex (MHC) molecule, critical for the defense system of the jawed vertebrates

• Project/Area Number: 19570223
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Fujita Health University
• Principal Investigator: HASHIMOTO Keiichiro Fujita Health University, 総合医科学研究所, 教授 (70192268)
• Co-Investigator(Kenkyū-buntansha): DIJKSTRA J・M 藤田保健衛生大学, 総合医科学研究所, 助教 (10387681) ; 山口 央輝 藤田保健衛生大学, 総合医科学研究所, 助教 (70319250)
• Co-Investigator(Renkei-kenkyūsha): DIJKSTRA J・M 藤田保健衛生大学, 総合医科学研究所, 助教 (10387681)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 免疫 / 分子進化 / 生体防御 / 魚類 / 遺伝子 / MHC / 獲得免疫系 / 有顎脊椎動物 / 進化 / ゲノム / 生体分子 / 免疫学 / 主要組織適合遺伝子複合体

Research Abstract

To elucidate the evolution of MHC molecules in the vertebrates, we tried to isolate novel MHC-related genes from various fish genome. Fish is the critical and the most primitive animal group in which the MHC-related genes could be found. We investigated MHC-related genes in the fish genome and also transcripts of those genes using RACE method. We also found some genes in the fish which can encode proteins with some MHC-related domains.

Research Products

• [Journal Article] Constitutive high expression of interleukin-4/13A and GATA-3 in gill and skin ofsalmonid fishes suggests that these tissues form Th2-skewed immune environments. (2011)
  • Author(s): Takizawa F, Koppang EO, Ohtani M, Nakanishi T, Hashimoto K, Fischer U, Dijkstra JM.
  • Journal Title: Mol.Immunol. (in press)
  • Peer Reviewed
• [Journal Article] Constitutive high expression of interleukin-4/13A and GATA-3 in gill and skin ofsalmonid fishes suggests that these tissues form Th2-skewed immune environments. (2011)
  • Author(s): Takizawa F
  • Journal Title: Mol.Immunol. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] A molecule in teleost fish, related with human MHC-encoded G6F, has a cytoplasmic tail with ITAM and marks the surface of thrombocytes and in some fishes also of erythrocytes. (2010)
  • Author(s): Ohashi K, Takizawa F, Tokumaru N, Nakayasu C, Toda H, Fischer U, Moritomo T, Hashimoto K, Nakanishi T, Dijkstra JM.
  • Journal Title: Immunogenetics 62 Pages: 543-559
  • Peer Reviewed
• [Journal Article] Zinc-dependent binding between peptides derived from rainbow trout CD8alpha and LCK. (2010)
  • Author(s): Hayashi N, Takeuchi M, Nakanishi T, Hashimoto K, Dijkstra JM.
  • Journal Title: Fish Shellfish Immunol. 28 Pages: 72-76
  • Peer Reviewed
• [Journal Article] A molecule in teleost fish, related with human MHC-encoded G6F, has a cytoplasmic tail with ITAM and marks the surface of thrombocytes and in some fishes also of erythrocytes. (2010)
  • Author(s): Ohashi K
  • Journal Title: Immunogenetics Volume: 62 Pages: 543-559
  • Peer Reviewed
• [Journal Article] Zincdependent binding between peptides derived from rainbow trout CD8al pha and LCK (2010)
  • Author(s): Hayashi N, Takeuchi M, Nakani shi T, Hashi moto K, Djkstra JM.
  • Journal Title: Fish Shellfish Immunol 28 Pages: 72-76
  • Peer Reviewed
• [Journal Article] Zinc-dependent binding between peptides derived from rainbow trout CD8alpha and LCK (2010)
  • Author(s): Hayashi N
  • Journal Title: Fish Shellfish Immunol 28 Pages: 72-76
  • Peer Reviewed
• [Journal Article] Comprehensive clarification of two paralogous interleukin 4/13 loci in teleost fish. (2008)
  • Author(s): Ohtani M, Hayashi N, Hashimoto K, Nakanishi T, Dijkstra JM.
  • Journal Title: Immunogenetics 60 Pages: 383-397
  • Peer Reviewed
• [Journal Article] Comprehensive clarification of two paralogous interleukin 4/13 loci in teleost fish (2008)
  • Author(s): Ohtani M, Hayashi N, Hashimoto K, Nakanishi T, Dijkstra JM.
  • Journal Title: Immunogenetics 60 Pages: 383-397
  • Peer Reviewed
• [Journal Article] Comprehensive clarification of two paralogousinterleukin 4/13 loci in teleostfish (2008)
  • Author(s): Ohtani, M.
  • Journal Title: Immunogenetics 60 Pages: 383-397
  • Peer Reviewed
• [Journal Article] A third lineage of major histocompatibility complex(MHC)class I in teleost fish;MHC class II-linkage and processed genes. (2007)
  • Author(s): Dijkstra, J. M.
  • Journal Title: Immunogenetics 59 Pages: 305-321
  • Peer Reviewed
• [Presentation] MHC class Ib分子MR1の細胞内相互作用分子の解析 (2010)
  • Author(s): 山口央輝
  • Organizer: 第33回 日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Thrombocytes and erythrocytes in teleost fish express a surface marker related to mammalian G6F with a cytoplasmic tail bearing an ITAM (2010)
  • Author(s): Dijkstra JM, et al.
  • Organizer: 第14回国際免疫学会議
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-23
• [Presentation] Thrombocytes and erythrocytes in teleost fish express a surface marker related to mammalian G6F with a cytoplasmic tail bearing an ITAM (2010)
  • Author(s): Dijkstra J
  • Organizer: 第14回 国際免疫学会議
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-23
• [Presentation] MHC in salmonid fish and its linkage associations with disease resistance and social behavior (2010)
  • Author(s): Dijkstra JM, et al.
  • Organizer: 第9回国際獣医免疫学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-08-17
• [Presentation] MHC in salmonid fish and its linkage associations with disease resistance and social behavior (2010)
  • Author(s): Dijkstra J
  • Organizer: 第9回 国際獣医免疫学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-08-17
• [Presentation] MHCクラスIb分子MR1のα1α2ドメインの発現と精製 (2009)
  • Author(s): 山口央輝
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Molecular characteristics of the leukocyte markers CD4-1, CD4-2, LAG-3, CD8alpha and CD8beta in teleost fish. (2009)
  • Author(s): Dijkstra JM, Hayashi N, Nakanishi T, Hashimoto K.
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 藤沢
  • Year and Date: 2009-08-04
• [Presentation] Molecular characteri stics of the Ieukocyte markers CD4-1, CD4-2, LAG-3, CD8al pha and CD8bet a in teleost fish (2009)
  • Author(s): Dijkstra JM
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 藤沢
  • Year and Date: 2009-08-04
• [Presentation] Molecular characteristics of the leukocyte markers CD4-1, CD4-2, LAG-3, CD8alpha and CD8beta in teleost fish (2009)
  • Author(s): Dijkstra JM
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 藤沢
  • Year and Date: 2009-08-04
• [Presentation] 強制発現細胞株におけるN末タグ付MR1分子の解析 (2008)
  • Author(s): 山口 央輝
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Evolution of cytokines important for T helper cell differentiation. (2008)
  • Author(s): Dijkstra JM, Ohtani M, Hayashi N, Nakanishi T, Hashimoto K.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Evolution of cytokines important for Thelper cell differentiation (2008)
  • Author(s): Dijkstra JM
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Major histocompatibility complex (MHC) linkage groupsand disease resistance in salmonid fishes ; a review (2008)
  • Author(s): Dijkstra, J. M.
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 東京
  • Year and Date: 2008-08-25
• [Presentation] Alternative splicing forms of the human MR1 gene. (2007)
  • Author(s): 山口 央輝
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] Unique features of major histocompatibility complex (MHC) genes in fish. (2007)
  • Author(s): Dijkstra JM, Hashimoto K.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Uhique features of majorhist ocompati bility complex (MHC) genes in fish (2007)
  • Author(s): Dijkstra JM
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Unique features of major histocompatibility complex(MHC)genes in fish. (2007)
  • Author(s): Dijkstra, J. M.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Remarks] ホームページ等
  • URL: http://www.fujita-hu.ac.jp/ICMS/res03.html
• [Remarks]
  • URL: http://www.fujita-hu.ac.jp/ICMS/res03.html