Genetic study on overexpression mechanisms of cytochrome P450 in insecticide resistant insect

• Project/Area Number: 19580062
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: TOMITA Takashi National Institute of Infectious Diseases, 昆虫医科学部, 室長 (20180169)
• Co-Investigator(Kenkyū-buntansha): KASAI Shinji 国立感染症研究所, 昆虫医科学部, 主任研究官 (80332360)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 殺虫剤抵抗性 / シトクロムP450 / 遺伝子過剰発現 / 遺伝子重複 / シス作用性調節変異 / ネッタイイエカ / ピレスロイド / シトクロムP450モノオキゲナーゼ / DNA増幅 / 殺虫剤解毒機構 / アカイエカ種群蚊 / シトウロムP450モノオキゲナーゼ

Research Abstract

A P450 gene Cyp9m10 is about 260-fold overexpressing in a pyrethroid-resistant strain of Culex quinquefasciatus compared to a susceptible strain. a region of approximately 100kb in length including the Cyp9m10 locus was specifically duplicated in the resistant strain. The two duplicated Cyp9m10 copies shared a completely identical sequence within the transcribed region and the flanking region up to the breakpoint located 1.1kb upstream of the transcriptional start site. A Miniature Inverted-repeat Transposable Element (MITE)-like element was specifically inserted 0.2kb upstream of both Cyp9m10 copies in the resistant strain. We obtained direct evidence that the Cyp9m10 overexpression is caused by a cis-acting mutation. In backcross experiment, a haplotype containing the two duplicated Cyp9m10 copies was strongly associated with the pyrethroid resistance.

Research Products

• [Journal Article] Genomic structures of Cyp9m10 in pyrethroid resistant and-susceptible strains of Culex quinquefasciatus. (2010)
  • Author(s): Kentaro Itokawa, Osamu Komagata, Shinji Kasai, Yoshika Okamura, Masahiro Masada, Takashi Tomita
  • Journal Title: Insect Biochemistry and Molecular Biology DOI#10.1016/j.ibmb.(in press)
  • Peer Reviewed
• [Journal Article] Overexpression of cytochrome P450 genes in pyrethroid-resistant Culex quinquefasciatus. (2010)
  • Author(s): Osamu Komagata, Shinji Kasai, Takashi Tomita
  • Journal Title: Insect Biochemistry and Molecular Biology 40(2) Pages: 146-152
  • Peer Reviewed
• [Journal Article] Overexpression of cytochrome P450 genes in pyrethroid-resistant Culex quinquefasciatus (2010)
  • Author(s): Komagata, O., Kasai, S., Tomita, T.
  • Journal Title: Insect Biochemistry and Molecular Biology 40 Pages: 146-152
  • Peer Reviewed
• [Journal Article] ACS Publications (2009)
  • Author(s): Komagata, O., Kasai, S., Tomita, T.
  • Journal Title: Insecticide Resistance in the Mosquito Culex pipiens Complex, In "Advances in Human Vector Control" Pages: 28-37
• [Journal Article] Cytochrome P450 monooxygenase-mediated permethrin resistance confers limited and larval specific cross-resistance in the southern house mosquito,Culex pipiens quinquefasciatus (2007)
  • Author(s): Hardstone MC, Leichter C, Harrington LC, Kasai S, Tomita T, Scott JG
  • Journal Title: Pesticide Biochemistry and Physiology 89 Pages: 184-191
  • Peer Reviewed
• [Presentation] 殺虫剤抵抗性蚊におけるシトクロムP450遺伝子の過剰発現 (2010)
  • Author(s): 冨田隆史
  • Organizer: 第54回日本応用動物昆虫学会大会研究小集会
  • Place of Presentation: 千葉大学
  • Year and Date: 2010-03-28
• [Presentation] 殺虫剤抵抗性と連鎖するCYP9M10遺伝子の過剰発現と進化 (2010)
  • Author(s): 糸川健太郎, (他6名)
  • Organizer: 第54回日本応用動物昆虫学会大会
  • Place of Presentation: 千葉大学
  • Year and Date: 2010-03-27
• [Presentation] ネッタイイエカ幼虫のピレスロイド抵抗性に関与する解毒酵素シトクロムP450 (CYP9M10)の遺伝学的研究 (2009)
  • Author(s): 糸川健太郎, (他4名)
  • Organizer: 第62回日本衛生動物学会東日本支部大会
  • Place of Presentation: 国立感染症研究所
  • Year and Date: 2009-10-17
• [Presentation] ネッタイイエカ幼虫のピレスロイド抵抗性に関与する解毒酵素シトクロムP450(CYP9M10)の遺伝学的研究 (2009)
  • Author(s): 糸川健太郎, 駒形修, 葛西真治, 政田正弘, 冨田隆史
  • Organizer: 第62回日本衛生動物学会東日本支部大会
  • Place of Presentation: 国立感染症研究所
  • Year and Date: 2009-10-17
• [Presentation] 殺虫剤抵抗性ネッタイイエカで高発現しているピレスロイド代謝性P450遺伝子の発現機構に関する研究 (2009)
  • Author(s): 糸川健太郎, (他5名)
  • Organizer: 第53回日本応用動物昆虫学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-03-30
• [Presentation] 殺虫剤抵抗性ネッタイイエカで高発現しているピレスロイド代謝性P450遺伝子の発現機構に関する研究 (2009)
  • Author(s): 糸川健太郎, 駒形修, 岡村佳香, 政田正弘, 冨田隆史
  • Organizer: 第53回 日本応用動物昆虫学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-03-30
• [Presentation] Insecticide resistance in the mosquito Culex pipiens complex (2008)
  • Author(s): Takashi Tomita, (他2名)
  • Organizer: 4th Pan Pacific Conference on Pesticide Science
  • Place of Presentation: Waikiki Beach Marriott Resort & Spa, Honolulu.
  • Year and Date: 2008-06-02
• [Presentation] Insecticide resistance in the mosquito Culex pipiens complex (2008)
  • Author(s): Tomita T, Komagata O, Kasai S
  • Organizer: 4th Pan Pacific Conference on Pesticide Sciences
  • Place of Presentation: Waikiki Beach Marriott Resort & Spa, Honolulu
  • Year and Date: 2008-06-02
• [Presentation] アカイエカ種群蚊の殺虫剤抵抗性及び亜種判別に関わる遺伝子型の個体別決定法 (2007)
  • Author(s): 駒形修, (他6名)
  • Organizer: 第59回日本衛生動物学会大会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2007-04-03
• [Presentation] 衛生害虫の殺虫剤抵抗性獲得の分子機構解明に関する研究 (2007)
  • Author(s): 冨田 隆史
  • Organizer: 第59回日本衛生動物学会大会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2007-04-03
• [Book] Advances in Human Vector Control.(Clark, J. M., Bloomquist, J. R., Kawada, H. (Eds.), Insecticide resistance in the mosquito Culex pipiens Complex) (2009)
  • Author(s): Osamu Komagata, Shinji Kasai, Takashi Tomita
  • Publisher: ACS Publications
• [Remarks]
  • URL: http://www.nih.go.jp/niid/entomology/manuscript.html
• [Remarks]
  • URL: http://www.nih.go.jp/niid/entomology/manuscript.html