Reconstitution of protein synthesis system of mammals
| Project/Area Number |
19580113
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | University of Hyogo (2008-2009)
The Institute of Physical and Chemical Research (2007) |
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Principal Investigator |
IMATAKA Hiroaki University of Hyogo, 大学院・工学研究科, 教授 (50201942)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | タンパク質合成 / 無細胞システム / 翻訳 / 翻訳因子 / 再構成 / ほ乳動物細胞 / セルフリー合成 / 翻訳開始 / ペプチド合成 |
|---|
| Research Abstract |
We purified mammalian translation initiation factors : eIF1, eIF1A, eIF2, eIF2B, eIF3, eIF4E, eIF4A, eIF4B, eIF4G, eIF5, eIF5B, elongation factors : eEF1A eEF1B・eEF2, termination factors : eRF1、eRF3, aminoacyl tRNA, and ribosome subunits (40S, 60S). By mixing all these factors, we successfully established a reconstituted protein synthesis system derived from mammals.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article]2010
Author(s)
Satoshi Mikami
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Journal Title
Cell-free protein production : Methods and Protocols(Human Press)
Pages: 43-52
Related Report
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[Journal Article]2009
Author(s)
今高寛晃
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Journal Title
ヒト細胞由来無細胞タンパク質合成システムの魅力(日本生化学会 生化学)
Pages: 303-307
Related Report
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[Journal Article]2009
Author(s)
今高寛晃
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Journal Title
翻訳開始の制御(共立出版 蛋百質 核酸 酵素)
Pages: 2213-2218
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