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Reconstitution of protein synthesis system of mammals

Research Project

Project/Area Number 19580113
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionUniversity of Hyogo (2008-2009)
The Institute of Physical and Chemical Research (2007)

Principal Investigator

IMATAKA Hiroaki  University of Hyogo, 大学院・工学研究科, 教授 (50201942)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsタンパク質合成 / 無細胞システム / 翻訳 / 翻訳因子 / 再構成 / ほ乳動物細胞 / セルフリー合成 / 翻訳開始 / ペプチド合成
Research Abstract

We purified mammalian translation initiation factors : eIF1, eIF1A, eIF2, eIF2B, eIF3, eIF4E, eIF4A, eIF4B, eIF4G, eIF5, eIF5B, elongation factors : eEF1A eEF1B・eEF2, termination factors : eRF1、eRF3, aminoacyl tRNA, and ribosome subunits (40S, 60S). By mixing all these factors, we successfully established a reconstituted protein synthesis system derived from mammals.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (35 results)

All 2010 2009 2008 2007 Other

All Journal Article (12 results) (of which Peer Reviewed: 9 results) Presentation (17 results) Book (5 results) Remarks (1 results)

  • [Journal Article] N-terminally truncated GADD34 proteins are convenient translation enhancers in a human cell-derived in vitro protein synthesis system.2010

    • Author(s)
      Satoshi Mikami, Tominari Kobayashi, Kodai Machida, Mamiko Masutani, Shigeyuki Yokoyama, Hiroaki Imataka
    • Journal Title

      Biotechnology letters DOI: 10. 1007/s10529-010-0251-7

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article]2010

    • Author(s)
      Satoshi Mikami
    • Journal Title

      Cell-free protein production : Methods and Protocols(Human Press)

      Pages: 43-52

    • Related Report
      2009 Annual Research Report
  • [Journal Article] General RNA-binding proteins have a function in poly(A)-binding protein-dependent translation2009

    • Author(s)
      Y.Svitkin
    • Journal Title

      EMBO J. 28

      Pages: 58-68

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Requirement of RNA-binding of Mammalian eIF4GI for Efficient Interaction of eIF4E with mRNA Cap2009

    • Author(s)
      A.Yanagiya
    • Journal Title

      Mol.Cell.Biol. 29

      Pages: 1661-1669

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the α subunit of human translation initiation factor 2B2009

    • Author(s)
      T.B.Hiyama
    • Journal Title

      J.Mol.Biol. 392

      Pages: 937-951

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The ELAV protein HuD stimulates cap-dependent translation in a poly(A)-and eIF4A-dependent manner2009

    • Author(s)
      A.Fukao
    • Journal Title

      Mol.Cell 36

      Pages: 1007-1017

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      今高寛晃
    • Journal Title

      ヒト細胞由来無細胞タンパク質合成システムの魅力(日本生化学会 生化学)

      Pages: 303-307

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      今高寛晃
    • Journal Title

      翻訳開始の制御(共立出版 蛋百質 核酸 酵素)

      Pages: 2213-2218

    • Related Report
      2009 Annual Research Report
  • [Journal Article] A human cell-derived in vitro coupled transcription/translation system2008

    • Author(s)
      Satoshi Mikami, Tominari Kobayashi, Mamiko Masutani, Shigeyuki Yokoyama, Hiroaki Imataka
    • Journal Title

      optimized for production of recombinant proteins Protein Expr. Purif 62

      Pages: 190-198

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A human cell-derived in vitro coupled transcription/translation system optimized for production of recombinant proteins2008

    • Author(s)
      Satoshi Mikami
    • Journal Title

      Protein Expr. Purif 62

      Pages: 190-198

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An improved cell-free system for picornavirus synthesis2007

    • Author(s)
      Tominari Kobayashi, Satoshi Mikami, Shigeyuki Yokoyama, Hiroaki Imataka
    • Journal Title

      J. Virol. Methods 142

      Pages: 182-188

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reconstitution reveals the functional core of mammalian eIF32007

    • Author(s)
      Mamiko Masutani
    • Journal Title

      EMBO J 26

      Pages: 3373-3383

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 試験菅内ウイルス粒子合成技術の開発2010

    • Author(s)
      今高寛晃
    • Organizer
      第14回けいはんなシーズフォーラム
    • Place of Presentation
      中ノ島センタービル、大阪市
    • Year and Date
      2010-01-26
    • Related Report
      2009 Final Research Report
  • [Presentation] 試験菅内ウイルス粒子合成技術の開発2010

    • Author(s)
      今高寛晃
    • Organizer
      第14回 けいはんなシーズフォーラム
    • Place of Presentation
      大阪市
    • Year and Date
      2010-01-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 感染細胞のウイルス合成を再現するヒト細胞由来無細胞タンパク質合成系の開発2009

    • Author(s)
      小林富成
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜市
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト細胞由来セルフリータンパク質合成システムを用いたタンパク質複合体の合成2009

    • Author(s)
      三上暁
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜市
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] 感染細胞のウイルス合成を再現するヒト細胞由来無細胞タンパク質合成系の開発2009

    • Author(s)
      小林富成
    • Organizer
      第4回 無細胞生命科学研究会
    • Place of Presentation
      下呂市
    • Year and Date
      2009-11-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] 真核細胞特異的/翻訳依存的タンパク質プロセシング:セルフリーシステムによる解析2009

    • Author(s)
      三上暁
    • Organizer
      第4回 無細胞生命科学研究会
    • Place of Presentation
      下呂市
    • Year and Date
      2009-11-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] A novel human in vitro translation system for glycoprotein expression2009

    • Author(s)
      K. Vattem, S. Mikami, K. Maas, E. Hommema, A. Morgan, M. Schofield, B. Webb, A. Deshpande, H. Imataka
    • Organizer
      第8回国際プロテオミクス会議
    • Place of Presentation
      トロント
    • Year and Date
      2009-09-28
    • Related Report
      2009 Final Research Report
  • [Presentation] Human Cell-Derived in vitro Coupled Transcription/Translation Systems2009

    • Author(s)
      S Mikami, K Machida S Yokoyama, H Imataka
    • Organizer
      第8回国際プロテオミクス会議
    • Place of Presentation
      トロント
    • Year and Date
      2009-09-28
    • Related Report
      2009 Final Research Report
  • [Presentation] Human Cell-Derived in vitro Coupled Transcription/Translation Systems2009

    • Author(s)
      今高寛晃
    • Organizer
      第8回 国際プロテオミクス会議
    • Place of Presentation
      トロント
    • Year and Date
      2009-09-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] A novel human in vitro translation system for glycoprotein expression2009

    • Author(s)
      K.Vattem
    • Organizer
      第8回 国際プロテオミクス会議
    • Place of Presentation
      トロント
    • Year and Date
      2009-09-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] セルフリーRNAレプリコンシステムを用いたRNAウイルスの翻訳とRNA複製メカニズムの解析2009

    • Author(s)
      小林富成
    • Organizer
      第11回日本RNA学会年会
    • Place of Presentation
      新潟市
    • Year and Date
      2009-07-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト細胞由来セルフリータンパク質合成システムの開発とその応用2008

    • Author(s)
      今高寛晃
    • Organizer
      大阪大学蛋白質研究所セミナー : 蛋白質合成法の最近の進歩と生命科学
    • Place of Presentation
      大阪大学
    • Year and Date
      2008-09-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] 動物細胞由来セルフリータンパク質合成系の確立2008

    • Author(s)
      三上暁、小林富成、舛谷真美子、横山茂之、今高寛晃
    • Organizer
      2008年度日本農芸化学会
    • Place of Presentation
      名城大学、名古屋
    • Related Report
      2009 Final Research Report
  • [Presentation] 動物細胞での共発現システムを用いたタンパク質複合体の再構成2007

    • Author(s)
      舛谷 真美子
    • Organizer
      第30回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] 強力なヒト細胞由来セルフリータンパク質合成システムの開発2007

    • Author(s)
      三上 暁
    • Organizer
      第30回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] 真核細胞翻訳開始因子eIF3のサブユニットの機能解析2007

    • Author(s)
      舛谷 真美子
    • Organizer
      第9回日本RNA学会年会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2007-07-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] 強力なヒト細胞由来セルフリータンパク質合成システムの開発2007

    • Author(s)
      三上暁、小林富成、横山茂之、今高寛晃
    • Organizer
      第30回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Related Report
      2009 Final Research Report
  • [Book] Cell-free protein synthesis systems with extracts from cultured human cells. In: Endo, Y., Takai, K. and Ueda, T. (Eds.), Methods in Molecular Biology 607, Cell-free protein production: Methods and Protocols.2010

    • Author(s)
      Satoshi Mikami, Tominari Kobayashi, Hiroaki Imataka
    • Publisher
      Human Press
    • Related Report
      2009 Final Research Report
  • [Book] 大腸菌抽出液を用いた無細胞タンパク質合成系, タンパク質科学実験法1, タンパク質をつくるー抽出・精製と合成(長谷俊治、高尾敏文、高木淳一、編集)2008

    • Author(s)
      横山茂之, 今高寛晃
    • Publisher
      化学同人
    • Related Report
      2009 Final Research Report
  • [Book] mrnaの調製ほ乳動物細胞由来in vitro翻訳システムRNA実験ノート2007

    • Author(s)
      今高寛晃
    • Publisher
      羊土社
    • Related Report
      2009 Final Research Report
  • [Book] 翻訳開始の制御

    • Author(s)
      今高寛晃、三上暁
    • Publisher
      蛋白質核酸酵素
    • Related Report
      2009 Final Research Report
  • [Book] ヒト細胞由来無細胞タンパク質合成システムの魅力

    • Author(s)
      今高寛晃、三上暁
    • Publisher
      生化学
    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.eng.u-hyogo.ac.jp/msc/msc15/index.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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