Analysis of the mechanism of sex determination and gonadal sexual differentiation of the pufferfish, Takifugu rubripes

• Project/Area Number: 19580207
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Kyushu University
• Principal Investigator: AKIHIKO Yamaguchi Kyushu University, 農学研究院, 助教 (10332842)
• Co-Investigator(Kenkyū-buntansha): MATSUYAMA Michya 九州大学, 農学研究院, 教授 (00183955)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 内分泌 / 配偶子形成 / 環境要因 / ステロイドホルモン / 水産 / 発生 / 分化 / 遺伝子 / 発生・文化

Research Abstract

In order to assess the involvement of aromatase and sex steroids in gonadal differntiation, an aromatase inhibitor (AI) was administered to developing pufferfish (torafugu) till the 100^<th> day after hatch. AI inhibits ovarian cavity formation, and then irreversible sex change (female to male) was observed. High temperature treatment, which is used for sex reversal of many fishes, induces gonadal degeneration in the pufferfish. We also show that aromatase expression can be regulated by gonadotropin using our newly developed in vitro gonad culture system.

Research Products

• [Journal Article] Estradiol-17βtreatment induces intersexual gonadal development in the pufferfish, Takifugu rubripes. (2009)
  • Author(s): Lee, K-H, Yamaguchi, A, Rashid, H, Kadomura, K, Yasumoto, S., Matsuyama, M
  • Journal Title: Zool. Sci. 26 Pages: 639-645
  • NAID: 40016807043
• [Journal Article] Germcell degeneration in high-temperature treated pufferfish, Takifugu rubripes. (2009)
  • Author(s): Lee, K-H, Yamaguchi, A, Bashid-H, Kadomura, K, Yasumoto, S., Matsuyama, M
  • Journal Title: Sex. Dev. 3 Pages: 225-232
• [Journal Article] Estradiol-17β treatment induces intersexual gonadal development in the pufferfish, Takifugu rubripes (2009)
  • Author(s): Lee, K-H, et al.
  • Journal Title: Zoological Science 26 Pages: 639-645
  • Peer Reviewed
• [Journal Article] Germ cell degeneration in high-temperature treated pufferfish, Takifugu rubripes (2009)
  • Author(s): Lee, K. H., et al.
  • Journal Title: Sexual Development 3(in press)
  • Peer Reviewed
• [Journal Article] 全オス化は可能か?トラフグの性決定・性分化研究 (2008)
  • Author(s): 山日明彦
  • Journal Title: 月刊養殖 7月号 Pages: 14-18
• [Journal Article] 全雄化は可能か? トラフグの性決定・性分化研究 (2008)
  • Author(s): 山口, 明彦
  • Journal Title: 養殖 45 Pages: 22-26
• [Journal Article] (Takifugu rubripes) sexual differentiation: CYP19 regulation and aromatase inhibitor induced testicular development. (2007)
  • Author(s): Fiashid, H, Kitano H, Lee, KH, Nii, S., Shigematsu, T., Kadomura, K, Yamaguchi, A, Matsuyama, M
  • Journal Title: Sex. Dev. 1 Pages: 311-322
• [Journal Article] Fugu(Takifugu rubripes)Sexual Differentiation:CYP19 Regulation and Aromatase Inhibitor Induced Tbsticular Development (2007)
  • Author(s): Rashid, H., et. al.
  • Journal Title: Sexual Development 1 Pages: 311-322
  • Peer Reviewed
• [Presentation] 魚類卵母細胞型ラミンB3のRSリピートを認識するキナーゼ、SRPK1の解析 (2009)
  • Author(s): 山口, 明彦
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 静岡県グランシップ
  • Year and Date: 2009-09-19
• [Presentation] Site-specific mutagenic study of the RS-rich motif in GV-lamin B3 to the identification of involvement of SRPK1 in phosphorylation of p34cdc2 target site in fish oocytes (2009)
  • Author(s): 山口, 明彦
  • Organizer: 第61回日本細胞生物学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-06-03
• [Presentation] Estradiol-17βtreatment induces ovarian development in testis of pufferfish, Takifugu rubripes. (2008)
  • Author(s): Lee, K-H, Yamaguchi, A, Rashid, H, Kitano, H, Nii, S., Sbigematsu, T., Kadomura, K, Yasumoto, S., Matsuyama, M.
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: 横浜
  • Year and Date: 2008-10-23
• [Presentation] Estradiol-17βtreatment induces ovarian development in testis of pufferfish, Takitugu rubripes (2008)
  • Author(s): Lee, K. H.
  • Organizer: 第5回世界水産学会議
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-10-23
• [Presentation] トラフグ精巣に存在する2種類のbrain-typeアロマターゼmRNAの発現と分布。 (2008)
  • Author(s): Rashid, H, Lee K-H, Matsuyama, M., Yamaguchi, A
  • Organizer: 第79回日本動物学会
  • Place of Presentation: 福岡大学
  • Year and Date: 2008-09-07
• [Presentation] トラフグ精巣に存在する2種類のbrain-typeアロマターゼmRNAの発現と分布 (2008)
  • Author(s): 山口, 明彦
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学
  • Year and Date: 2008-09-07
• [Presentation] 卵母細胞型ラミンB3をリン酸化する硬骨魚類SRキナーゼの生化学的解析 (2008)
  • Author(s): 小川, 真理子
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学
  • Year and Date: 2008-09-06
• [Presentation] Modulation of aromatase activity results in masculinization of Japanese tiger puffer Takifugu rubripes. (2008)
  • Author(s): Rashid H, Yamaguchi A, Matsuyama M.
  • Organizer: 15th International Gongress on Biotechnology in Animal Reproduction
  • Place of Presentation: Mymensingh, Bangl adesh
  • Year and Date: 2008-08-06
• [Presentation] Characterization of SR-kinases interact with RS-rich motif on germinal vesicle lamin B3 in teleosts (2008)
  • Author(s): 山口, 明彦
  • Organizer: 第60回日本細胞生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-06-29
• [Presentation] Effects of exogenous factors on gonadal sex differertiation and development of fugu Takifugu rubripes. (2008)
  • Author(s): Rashid H, Lee K-H, Kadomura K, Yamaguchi, A, Matsuyama, M.
  • Organizer: World Aquacul ture 2008
  • Place of Presentation: 釡山
  • Year and Date: 2008-05-19
• [Presentation] Effects of exogenous factors on gonadal sex differentiation and development of fugu Talkifugu rubripes (2008)
  • Author(s): Rashid H.
  • Organizer: 世界水産養殖会議 (World aquaculture)
  • Place of Presentation: 釜山
  • Year and Date: 2008-05-19
• [Presentation] 未成熟期トラフグの脳下垂体における生殖腺刺激ホルモン(GtH)mRNAの発現解析。 (2008)
  • Author(s): 重松亨・李敬勲・新居早也佳・Harunur Rashid・山口明彦・松山倫也
  • Organizer: 平成19年度日本水産学会九州支部大会
  • Place of Presentation: 宮崎大学
  • Year and Date: 2008-01-26
• [Presentation] トラフグ精巣セルトリ細胞の培養系の開発 (2008)
  • Author(s): 新居早也佳・李敬勲・山日明彦・松山倫也
  • Organizer: 平成19年度日本水産学会九州支部大会
  • Place of Presentation: 宮崎大学
  • Year and Date: 2008-01-26
• [Presentation] トラフグ精巣セルトリ細胞の培養系の開発 (2008)
  • Author(s): 新居 早也佳
  • Organizer: 平成19年度日本水産学会九州支部会
  • Place of Presentation: 官崎大学
  • Year and Date: 2008-01-26
• [Presentation] 未成熟期トラフグの脳下垂体における生殖腺刺激ホルモン(GtH)mRNAの発現解析 (2008)
  • Author(s): 重松 亨
  • Organizer: 平成19年度日本水産学会九州支部会
  • Place of Presentation: 宮崎大学
  • Year and Date: 2008-01-26
• [Presentation] Production of all male population of torafugu(Takifugu rubripes)using an aromatase inhibitor,fadrozole (2007)
  • Author(s): Rashid, H.
  • Organizer: The 4^<th> International Joint Symposium between Japan and Korea
  • Place of Presentation: 鳥取大学
  • Year and Date: 2007-11-28
• [Presentation] Production of all male population of torafugu (Takifugu rubripes) using an aromatase inhibitor (2007)
  • Author(s): Rashid, H, Kitano, H, Lee, K, H, Nii, S., Shigematsu, T., Yamaguchi, A, Matsuyama, M.
  • Organizer: fadrozole The 4th International Joint Symposium betveen Japan and Korea
  • Place of Presentation: 鳥取大学
• [Remarks]
  • URL: http://www.agr.kyushu-u.ac.jp/asweb/suil/lmb.html
• [Remarks]
  • URL: http://www.agr.kyushu-u.ac.jp/asweb/suil/lmb.html
• [Remarks]
  • URL: http://www.agr.kyushu-u.ac.jp/asweb/sui1/lmb.html
• [Remarks]
  • URL: http://www.agr.kyushu-u.ac.jp/asweb/sui1/lmb.html