Comparative analysis of Glycyrrhiza plants based on genotypes and chemical variations

• Project/Area Number: 19590121
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: Iwate Medical University
• Principal Investigator: HAYASHI Hiroaki Iwate Medical University, 薬学部, 准教授 (50260998)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 薬用資源学 / 遺伝子型 / 成分変異 / 甘草 / 成分育種 / 国際研究者交流 / 中国

Research Abstract

Comparative analysis of Glycyrrhiza plants based on the DNA sequence and chemical constituents were performed to characterize the variation of G. uralensis, which is one of the most important medicinal plants in the world. G.uralensis was divided into the two types, China-type and Kazakstan-type, based on the DNA sequences (pkr genotype) and chemical constituents in the leaves (chemotype). There was correlation between the pkr genotype and chemotype of G.uralesis. Furthermore, high glycyrrhizin-producing strains (T628 and 01A07-1) and a glycyrrhizin-deficient stain (83-555) were selected from the populations of G.uralensis.

Research Products

• [Journal Article] Economic importance of licorice (2009)
  • Author(s): H. Hayashi, H. Sudo
  • Journal Title: Plant Biotechnology 26 Pages: 101-104
  • NAID: 10025181445
• [Journal Article] 日局定量法を用いた簡便な甘草の基原植物推定法の検討 (2009)
  • Author(s): 田村, 高橋, 寺崎, 田中, 永井, 林藤井, 横田
  • Journal Title: 富山県薬事研究所年報 36 Pages: 46-53
• [Journal Article] (2009)
  • Author(s): Hiroaki Hayashi
  • Journal Title: Recent Advances in Plant Biotechnology, Chapter 5 Molecular Biology of Secondary Metabolism : Case Study for Glycyrrhiza Plants.(分担執筆Ed by Kirakosyan and Kaufman)(Springer) Pages: 89-103
• [Journal Article] カンゾウ属植物の自生地調査と系統解析 (2007)
  • Author(s): 林宏明
  • Journal Title: Foods & Food Ingredients Journal of Japan 212 Pages: 357-364
• [Journal Article] Molecular biology of triterpenoid biosynthesis in Glycyrrhiza plants (2007)
  • Author(s): H. Hayashi
  • Journal Title: Japanese Journal of Plant Science 1 Pages: 20-24
• [Journal Article] カンゾウ属植物の自生地調査と系統解析 (2007)
  • Author(s): 林 宏明
  • Journal Title: Foods & Food Ingredients Journal of Japan 212 Pages: 357-364
• [Presentation] 遺伝子配列によるカンゾウの系統解析 (2008)
  • Author(s): 林宏明
  • Organizer: 第4回甘草に関するシンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2008-06-28
• [Presentation] Biosynthesis of triterpene saponins in intact plant of Glycyrrhiza glabra (2008)
  • Author(s): H. Hayashi
  • Organizer: Shanghai International Synposium on Parmacy
  • Place of Presentation: Shanghai, China
• [Presentation] Comparative analysis of Glycyrrhiza uralensis (2007)
  • Author(s): H. Hayashi
  • Organizer: Asian Symposium for Pharmaceutical Science in JSPS Asian Core Program
  • Place of Presentation: Sasebo, Japan
• [Presentation] Comparative Analysis of Glycyrrhiza uralensis (2007)
  • Author(s): Hiroaki Hayashi
  • Organizer: Asian Symposium for Pharmaceutical Science In JSPS Asian Core Program
  • Place of Presentation: Sasebo,Nagasaki,Japan
• [Book] Recent Advances in Plant Biotechnology(ed by A. Kirakosyan and P. B. Kaufman, Chaptor 5, Molecular biology of secondary metabolism: Case study for Glycyrrhiza plants) (2009)
  • Author(s): H. Hayashi
  • Publisher: Springer
• [Book] Analysis of Natural Glycosides"(ed by Y. Shoyama, Comparative analysis of Glycyrrhiza plants based on triterpene saponin constituents and DNA sequences) (2007)
  • Author(s): H. Hayashi
  • Publisher: Research Signpost