| Project/Area Number |
19590194
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Jichi Medical University |
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Principal Investigator |
YASHIRO Takashi Jichi Medical University, 医学部, 教授 (80119859)
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| Co-Investigator(Kenkyū-buntansha) |
KIKUCHI Motoshi 自治医科大学, 医学部, 准教授 (60332988)
TAKIGAMI Syu 自治医科大学, 医学部, 講師 (60333366)
FUJIWARA Ken 自治医科大学, 医学部, 助教 (00382945)
HORIGUCHI Kotaro 自治医科大学, 医学部, 助教 (10409477)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 組織学 / 解剖学 / 細胞・組織 / シグナル伝達 / 糖鎖 / 下垂体 / ホルモン / 細胞接着 |
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| Research Abstract |
This study is focused on identifying the mechanisms regulating cell-cell affinities in anterior pituitary gland. In the gland, 5 kinds of hormone-producing cells are distributed in an intriguing manner. For example, LH/FSH cells are often surrounded by prolactin cells, while ACTH cells are located adjacent to GH cells very frequently. Further, folliculostellate cells, which does not produce hormone, have a propensity to adhere each other. Based on these observations, we hypothesized that there is signal transductions between these cell types through the cell adhesions, and live cell purification and identification are crucial to decipher the precise mechanism. Recently, we have established two different methods utilizing lectin and genetically modified S100-b-GFP rats, which allow us to purify and identify live hormone producing cells and live folliculostellate cells from the mixed population of anterior pituitary cells, respectively. The data obtained from two approaches suggested that there are three local regulatory mechanisms in anterior pituitary cells; matricrine (effect of matrix), juxtacrine (effect of cell adhesion molecule), and paracrine (local expression of retinaldehyde dehydrogenases).
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