Reconstruction of the benzodiazepine screening using the new technologies of mass spectrometry
| Project/Area Number |
19590682
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Nippon Medical School |
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Principal Investigator |
HAYASHIDA Makiko Nippon Medical School, 医学部, 准教授 (60164977)
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| Co-Investigator(Kenkyū-buntansha) |
TERADA Masaru 東邦大学, 医学部, 准教授 (30104649)
KUDO Keiko 九州大学, 医学研究科, 講師 (10186405)
KURISAKI Emiko 福島県立医科大学, 医学部, 講師 (30106356)
UEKUSA Kyoko 日本医科大学, 医学部, テクニカルスタッフ (50409215)
YAMAGUCHI Koji 日本医科大学, 医学部, 助教 (90465344)
仁平 信 日本医科大学, 医学部, 准教授 (40089636)
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| Project Period (FY) |
2007 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ベンゾジアゼピン系薬物 / 質量分析 / LC-TOF / LC-MS-MS / GC-MS / スクリーニング / 生体試料 / 固相抽出 |
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| Research Abstract |
Toxicological analysis with mass chromatography-spectrometry has spread widely and quickly with the progress in ionizing methods. This study was carried out by including new developments in mass chromatography-spectrometry research for forensic toxicology. Time-of-flight (TOF) mass spectrometry (MS) coupled with liquid chromatography (LC) has been developed for screening and for determining benzodiazepines with an exact mass database. All benzodiazepines have similar chemical structures and molecular weights, and, thus, show similar mass spectra and protonated molecule ions. Discrimination of mass spectrometry at low resolving power with LC-MS is usually difficult.
Benzodiazepines are widely used in medical practice in Japan, and data acquired from LC/TOF-MS measurements of 130 drugs, including benzodiazepines, were used to create an exact mass database. This database comprises molecular formulae, calculated exact masses, retention times and calibrations. In the analysis of samples from patients who had taken many drugs, selectivity was improved when the LC/TOF-MS exact mass database was used.
Comprehensive screening with LC/quadrupole TOF is the most effective method in forensic toxicology for analyzing the samples from cases of multidrug overdose. New developmental mass chromatography-spectrometry equipment is required because of the more precise and exact analyses in forensic toxicology.
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Report
(6 results)
Research Products
(61 results)
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[Presentation] 急性中毒症例の予後推定2009
Author(s)
白川洋一, 奈良女昭, 林田真喜子, 福本真理子, 福家千昭, 堀寧, 波多野弥生, 野村奈央, 飯塚富士子, 黒木由美子, 遠藤容子, 吉岡敏治
Organizer
第31回日本中毒学会総会・学術集会
Place of Presentation
東京
Related Report
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