Analysis of the mechanism of gastric inflammation caused by Helicobacter pylori infection and development of alternative medicine

• Project/Area Number: 19590696
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KITA Masakazu Kyoto Prefectural University of Medicine, 医学研究科, 准教授 (60153087)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: ヘリコバクター / サイトカイン / 耐性菌 / 漢方薬 / 代替療法

Research Abstract

Helicobacter pylori (H.pylori) is the major cause of chronic active gastritis and peptic ulcer disease. Although it is suspected that cytokines are involved in gastric inflammation induced by H.pylori, the mechanisms have not been clearly defined. In this study, the contributions of IL-17 to the induction of gastric inflammation and to the protection from H.pylori infection were investigated using IL-17 gene-knockout (IL-17^<-/->) mice. Our results suggest that IL-17 may play an important role in the inflammatory response to the H.pylori infection and ultimately influence the outcome of the H.pylori associated disease. On the other hand, the antibacterial effect of 18 herbal medicines was evaluated in vitro and in vivo. Rhei Rhizoma, Artemisiae Capillari Flos and Caryophylli Flos were bactericidal against H.pylori without the development of acquired resistance, suggesting that herbal medicines may have potential as complementary and alternative medicines for treatment of antibiotic-resistant H. pylori infection.

Research Products

• [Journal Article] Prevalence of Helicobacter pylori infection in long-term hemodialysis patients. (2009)
  • Author(s): Sugimoto M, Sakai K, Kita M, Imanishi J, Yamaoka Y
  • Journal Title: Kidney Int 75(1) Pages: 96-103
  • Peer Reviewed
• [Journal Article] Prevalence of Helicobacter pylori infection in long-term hemodialysis patients. (2009)
  • Author(s): Sugimoto M, Sakai K, Kita M, Imanishi J, Yamaoka Y.
  • Journal Title: Kidney Int. 75(1) Pages: 96-103
  • Peer Reviewed
• [Journal Article] Levels of interleukin-18 are markedly increased in Helicobacter pylori-infected gastric mucosa among patients with specific IL18 genotypes. (2008)
  • Author(s): Sakai K, Kita M, Sawai N, Shiomi S, Sumida Y, Kanemasa K, Mitsufuji S, Imanishi J, Yamaoka Y
  • Journal Title: J Infect Dis 197 Pages: 1752-1761
  • Peer Reviewed
• [Journal Article] IL-17 is involved in Helicobacter pylori-induced gastric inflammatory responses in a mouse model. (2008)
  • Author(s): Shiomi S, Toriie A, Imamura S, Konishi H, Mitsufuji S, Iwakura Y, Yamaoka Y, Ota H, Yamamoto T, Imanishi J, Kita M
  • Journal Title: Helicobacter 57 Pages: 518-524
  • Peer Reviewed
• [Journal Article] Levels of interleukin- 18 are markedly increased in Helicobacter pylori-infected gastric mucosa among patients with specific IL18 genotypes (2008)
  • Author(s): Sakai K, Kite M, Sawai N, Shiomi S, Sumida Y, Kanemasa K, Mitsufuji S, Imanishi J, Yamaoka Y.
  • Journal Title: J Infect Dis. 197 Pages: 1752-1761
  • Peer Reviewed
• [Journal Article] IL-17 is involved in Helicobacter pylori-Induced Gastric Inflammatory Responses in a Mouse Model (2008)
  • Author(s): Shiomi S, Toriie A, Imamura S, Konishi H, Mitsufuji S, Iwakura Y, Yamaokd Y, Ota H, Yamamoto T, Imanishi J, Kita M
  • Journal Title: Helicobacter 57 Pages: 518-524
  • Peer Reviewed
• [Journal Article] Functional and intracellular signaling differences associated with the Helicobacter pylori AlpAB adhesin from Western and East Asian strains. (2007)
  • Author(s): Lu H, Wu JY, Beswick EJ, Ohno T, Odenbreit S, Haas R, Reyes VE, Kita M, Graham DY, Yamaoka Y
  • Journal Title: J Biol Chem 282 Pages: 6242-6254
  • Peer Reviewed
• [Journal Article] Functional and intracellular signaling differences associated with the Helicobacter pylori AlpAB adhesin from Western and East Asian strains. (2007)
  • Author(s): Lu H, Wu JY, Beswick EJ, Ohno T, Odenbreit S, Haas R, Reyes VE, Kita M, Graham DY, Yamaoka Y.
  • Journal Title: J Biol Chem. 282 Pages: 6242-6254
  • Peer Reviewed
• [Presentation] Helicobacter pylori感染症におけるIL-17Aの関与 (2009)
  • Author(s): 喜多正和、塩見聡、今西二郎
  • Organizer: 第170回日仏生物学会
  • Place of Presentation: 東京
  • Year and Date: 2009-06-13
• [Presentation] ヘリコバクターピロリ誘発胃炎マウスモデルにおけるIL-17の役割 (2008)
  • Author(s): 喜多正和、塩見聡、今西二郎
  • Organizer: 日本実験動物学会
  • Place of Presentation: 仙台
  • Year and Date: 2008-05-17
• [Presentation] Helicobacter pylori感染により発症する胃炎におけるIL-17の役割 (2007)
  • Author(s): 喜多正和、塩見聡、今西二郎
  • Organizer: 日本インターフェロン・サイトカイン学会
  • Place of Presentation: 京都
  • Year and Date: 2007-07-06