A novel mouse model of diabetic nephropathy : MafA-deficient and beta cell-specific MafK-overexpressing hybrid transgenic

• Project/Area Number: 19590933
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: University of Tsukuba
• Principal Investigator: YOH Keigyou University of Tsukuba, 大学院・人間総合科学研究科, 准教授 (90323302)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 糖尿病 / 糖尿病性腎症 / Maf群タンパク質 / 転写因子 / Maf

Research Abstract

We generated hybrid transgenic mice that were MafA-deficient and also overexpressed MafK specifically in beta cells (MafA-/-MafK+). MafA-/-MafK+ mice developed severe overt diabetes mellitus within 5 weeks old. Furthermore, after uninephrectomy, these mice demonstrated three characteristics of human diabetic nephropathy : diffuse, nodular, and exudative lesions. MafA-/-MafK+ mice might be a useful model for the analysis of human diabetic nephropathy.

Research Products

• [Journal Article] MafA-deficient and beta cell-specific MafK-overexpressing hybrid transgenic mice develop human-like severe diabetic nephropathy (2009)
  • Author(s): Shimohata H, Yoh K, Fujita A, Morito N, Ojima M, Tanaka H, Hirayama K, Kobayashi M, Kudo T, Yamagata K, Takahashi, S.
  • Journal Title: Biochem Biophys Res Commun. 389(2) Pages: 235-40
  • Peer Reviewed
• [Journal Article] MafA-deficient and beta cell-specific MafK-overexpressing hybrid transgenic mice develop human-like severe diabetic nephropathy (2009)
  • Author(s): H.Shimohata, K.Yoh, et al.
  • Journal Title: Biochemical and Biophysical Research Communications Vol.389 Pages: 235-240
  • Peer Reviewed
• [Presentation] MafAKO : MafKTg compound mouse は重篤な糖尿病性腎症を呈する (2009)
  • Author(s): 藤田亜紀子, 楊景堯, 下畑誉, 森戸直記, 山縣邦弘
  • Organizer: 第1回 Advans 研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-12-26
• [Presentation] MafAKO : MafKTg compound mouseは重篤な糖尿病性腎症を呈する (2009)
  • Author(s): 藤田亜紀子、楊景堯, 他
  • Organizer: 第1回Advans研究会
  • Place of Presentation: 名古屋マリオットアソシナホテル(愛知県)
  • Year and Date: 2009-12-26
• [Presentation] MafA-deficient/MafK transgenic hybrid mice develop severe diabetic nephropathy (2007)
  • Author(s): N. Morito, H. Shimohata, Y. Hashimoto, K. Yoh, K. Yamagata, S. Takahashi
  • Organizer: American Society of Nephrology Renal Week
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2007-11-02
• [Presentation] MafA-deficient/MafK transgenic hybrid mice develop severe diabetic nephropathy (2007)
  • Author(s): N. Morito, K. Yoh, 他
  • Organizer: American Society of Nephrology Renal Week
  • Place of Presentation: San Francisco
  • Year and Date: 2007-11-02