Study on the mechanisms of human placental trophoblast syncytialisation : implication for the pathogenesis of pre-eclampsia

• Project/Area Number: 19591895
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Hiroshima University
• Principal Investigator: KUDO Yoshiki Hiroshima University, 大学院・医歯薬学総合研究科, 教授 (80241082)
• Co-Investigator(Kenkyū-buntansha): MIYOSHI Hiroshi 広島大学, 大学院・医歯薬学総合研究科, 講師 (40294590) ; NOBUZANE Takahiro 広島大学, 病院, 助教 (80403559)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 胎盤 / トロフォブラスト / 妊娠高血圧症候群

Research Abstract

We have demonstrated that CD98 surface antigen, syncytin, an envelope gene of HERV-W (human endogenous retrovirus-W), and its receptor type D mammalian retrovirus receptor (ASCT2) are involved in the process of cell fusion necessary for syncytiotrophoblast formation using a cell model of syncytialisation (the cytotrophoblast cell line BeWo following increased intracellular cAMP by forskolin treatment).

Research Products

• [Journal Article] Effects of menopause and hormone therapy on erythrocyte deformability. (2009)
  • Author(s): Sakashita T, Nobuzane T, Miyoshi H, Fujiwara H, Kudo Y
  • Journal Title: Menopause 16 Pages: 555-558
  • Peer Reviewed
• [Journal Article] Enhanced expression of P2X4 and P2X7 purinergic receptors in the myometrium of pregnant rats in preterm delivery models. (2009)
  • Author(s): Urabe S, Miyoshi H, Fujiwara H, Yamaoka K, Kudo Y
  • Journal Title: Reprod Sci. 16 Pages: 1186-1192
  • Peer Reviewed
• [Journal Article] Gene expression, profiling of advanced-stage serous ovarian cancers distinguishes novel subclasses and implicates ZEB2 in tumor progression and prognosis (2009)
  • Author(s): Yoshihara K
  • Journal Title: Cancer Science 100 Pages: 1421-1428
  • Peer Reviewed
• [Journal Article] Effects of menopause and hormone therapy on erythrocyte deformability (2009)
  • Author(s): Sakashita T
  • Journal Title: Menopause 16 Pages: 555-558
  • Peer Reviewed
• [Journal Article] Enhanced expression of P2X4 and P2X7 purinergic receptors in the myometrium of pregnant rats in preterm delivery models (2009)
  • Author(s): Urabe S
  • Journal Title: Reproductive Science 16 Pages: 1186-1192
  • Peer Reviewed
• [Journal Article] Morphologic effects of epithelial ion channels on the mouse uterus: differences between raloxifene analog (LY117018) and estradiol treatments. (2008)
  • Author(s): Nobuzane T, Tashiro S, Kudo Y
  • Journal Title: Am. J. Obstet. Gynecol 199
  • Peer Reviewed
• [Journal Article] Cell-free fetal DNA in the non-pregnant woman with thyroid disease disappeared after surgery. (2008)
  • Author(s): Tanigawa M, Miyoshi H, Samura O, Sugino K, Fujito N, Hyodo M, Miharu N, Kudo Y
  • Journal Title: Clin Chim Acta 397 Pages: 101-102
  • Peer Reviewed
• [Journal Article] Morphologic effects of epithelial ion channels on the mouse uterus : differences between raloxifene analog (LY117018) and estradiol treatments (2008)
  • Author(s): Nobuzane T
  • Journal Title: Am. J. Obstet. Gynecol. 199
  • Peer Reviewed
• [Journal Article] Cell-free fetal DNA in the non-pregnant woman with thyroiddisease disappeared after surgery (2008)
  • Author(s): Tanigawa M
  • Journal Title: Clin. Chim. Acta 397 Pages: 101-102
  • Peer Reviewed
• [Journal Article] Heterodimeric amino acid transporters in the placenta. (2007)
  • Author(s): Regnault T, Kudo Y, Glazier J, Roos S, Lewis R, Jansson T
  • Journal Title: Placenta 28
  • Peer Reviewed
• [Journal Article] Growth factors and trophoblast differentiation. (2007)
  • Author(s): Morrish DW, Kudo Y, Caniggia I, Cross J, Evain-Brion D, Gasperowicz M, Kokozidou M, Leisser C, Takahashi K, Yoshimatsu J
  • Journal Title: Placenta 28
  • Peer Reviewed
• [Journal Article] Heterodimeric amino acid transporters in the placenta (2007)
  • Author(s): Regnault T
  • Journal Title: Placenta 28
  • Peer Reviewed
• [Journal Article] Growth factors and trophoblast differentiation (2007)
  • Author(s): Morrish DW
  • Journal Title: Placenta 28
  • Peer Reviewed
• [Presentation] ヒト絨毛癌細胞(BeWo)の細胞融合に及ぼすsyncytinとそのレセプター(ASCT2)の影響 (2009)
  • Author(s): 信実孝洋, 松山聖, 工藤美樹
  • Organizer: 第17回日本胎盤学会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-17
• [Presentation] RNA interference-induced reduction in ASCT2 expression suppresses cell fusion of human placental BeWo cells. (2009)
  • Author(s): Nobuzane T, Matsuyama S, Kudo Y
  • Organizer: International Federation of Placental Association Meeting 2009
  • Place of Presentation: Seggau Adelaide, Australia
  • Year and Date: 2009-10-08
• [Presentation] RNA interference-induced reduction in ASCT2 expression suppresses cell fusion of human placental Be Wo cells (2009)
  • Author(s): Nobuzane T
  • Organizer: International Federation of Placental Association Meeting 2009
  • Place of Presentation: Adelaide, Australia
  • Year and Date: 2009-10-08
• [Presentation] Circulating endothelial progenitor cells and placental abruption. (2009)
  • Author(s): Sakashita T, Kudo Y
  • Organizer: International Society for the Study of Hypertension in Pregnancy 2009
  • Place of Presentation: Oxford, UK
  • Year and Date: 2009-09-12
• [Presentation] ヒト胎盤トロフォブラスト-その多彩な機能について- (2008)
  • Author(s): 工藤美樹
  • Organizer: 第16回日本胎盤学会学術集会
  • Place of Presentation: 浜松市
  • Year and Date: 2008-11-13
• [Presentation] RNA interference-induced reduction in CD98 expression suppresses cell fusion during syncytialisation of human placental BeWo cells. (2008)
  • Author(s): Nobuzane T, Kudo Y
  • Organizer: International Federation of Placental Association Meeting 2008
  • Place of Presentation: Seggau Castle, Austria
  • Year and Date: 2008-09-12
• [Presentation] RNA interference-induced reduction in CD98 expression suppresses cell fusion during syncytialisation of human placental BeWo cells (2008)
  • Author(s): Nobuzane T
  • Organizer: International Federation of Placental Association Meeting 2008
  • Place of Presentation: Seggau Castle, Austria
  • Year and Date: 2008-09-12
• [Presentation] Hypoxia suppresses expression of syncytin and cell fusion during syncytialisation of human placental BeWo cells: implications for impaired trophoblast syncytialisation in pre-eclampsia (2007)
  • Author(s): Kudo Y
  • Organizer: 14th World Congress on Gestational Trophoblastic Diseases
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2007-11-14
• [Presentation] Hypoxia suppresses expression of syncytin and cell fusion during syncytialisation of human placental BeWo cells: implications for impaired trophoblast syncytialisation in pre-eclampsia. (2007)
  • Author(s): Kudo Y
  • Organizer: 14th World Congress on Gestational Trophoblastic Diseases
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2007-11-14
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/sanfu/
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/sanfu/
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/sanfu/