Roll of interleukin-32 in inflammatory bone resorption caused by periodontopathic bacterium

• Project/Area Number: 19592123
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: TSUJISAWA Toshiyuki Kyushu Dental College, 歯学部, 助教 (60265006)
• Co-Investigator(Kenkyū-buntansha): NISHIHARA Tatsuji 九州歯科大学, 歯学部, 教授 (80192251) ; INOUE Hiromasa 九州歯科大学, 歯学部, 准教授 (20137326)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 炎症 / サイトカイン / インターロイキン / リポ多糖 / 自然免疫 / 歯周病原性細菌 / 骨代謝 / 歯周病原細菌

Research Abstract

We examined whether lipopolysaccaride (LPS) derived from Aggregatibacter actinomycetemcomitans, one of periodontopathic bacteria, induce expression of interlukin-32. We also analyzed the induction mechanism of IL-32 in human monocyte. Scince an inhibitor of NF-kB, one of downstream signaling of Toll-like receptor 4, inhibits the expression of IL-32, it was suggested that NF-kB activation was involved in the induction of IL-32 by LPS.

Research Products

• [Journal Article] Imflammatory response in epitherial cells induced by mechanical stress is supressed by hyaluronic acid. (2010)
  • Author(s): Toshinaga A, Hosokawa R., Okinaga T, Masaki C, Tsujisawa T, Nishihara T
  • Journal Title: Inflamation and Regeneration. 30(2)(in press)
  • Peer Reviewed
• [Journal Article] Heparin inhibits BMP-2 osteogenic bioactivity by binding to both BMP-2 and BMP receptor (2008)
  • Author(s): Kanzaki S, Takahashi T, Kanno T, Ariyoshi W, Shinmyouzu K, Tujisawa T, Nishihara T.
  • Journal Title: J Cell Physiol. 216(3) Pages: 844-50
  • Peer Reviewed
• [Journal Article] Mechanical stress-mediated Runx2 activation is dependent on Ras/ERK1/2 MAPK signaling in osteoblasts. (2007)
  • Author(s): Kanno T, Takahashi T, Tsujisawa T, Ariyoshi W, Nishihara T.
  • Journal Title: J Cell Biochem. 101(5) Pages: 1266-77
  • Peer Reviewed
• [Journal Article] Effects of lipopolysaccharide on newly established rat dental pulp-derived cell line with odontoblastic properties. (2007)
  • Author(s): Nomiyama K, Kitamura C, Tsujisawa T, Nagayoshi M, Morotomi T, Terashita M, Nishihara T.
  • Journal Title: J Endod. 33(10) Pages: 1187-91
  • Peer Reviewed
• [Journal Article] Role of caspases in cleavage of lamin A/C and PARP during apoptosis in macrophages infected with a periodontopathic bacterium. (2007)
  • Author(s): Okinaga T, Kasai H, Tsujisawa T, Nishihara T.
  • Journal Title: J Med Microbiol. 56(10) Pages: 1399-404
  • Peer Reviewed
• [Presentation] Establishment of rat dental pulp cell line showing odontblas- tic phenotype. (2007)
  • Author(s): Nomiyama K., Kitamura C., Tsujisawa T., Morotomi T., Nagayosi M., Terashita M., Nishihara T.
  • Organizer: 85th General Session of the International Association for Dental Research, New Orleans
• [Presentation] The effect of glycosamino- glucans on BMP Activity. (2007)
  • Author(s): Kanzaki S., Kanno T., Ariyoshi W., Shinmyozu K., Tsujisawa T., Takahashi T., Nishihara T.
  • Organizer: 85th General Session of the International Associati- on for Dental Research, New Orleans