Development of new therapy of oral infection and cancer by applying innate immunity network.

• Project/Area Number: 19592309
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: OKUMURA Kazuhiko Health Sciences University of Hokkaido, 歯学部, 講師 (60194510)
• Co-Investigator(Kenkyū-buntansha): ISOGAI Emiko 北海道医療大学, 歯学部, 講師 (80113570)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 自然免疫分子 / カセリシジン・ファミリー / hCAP18 / LL-37 / 口腔感染症 / 口腔扁平上皮癌 / アポトーシス / ネクローシス / 樹状細胞 / 口腔扁平上皮癌細胞 / 抗菌ペプチド / 抗腫瘍活性 / 抗菌効果 / 細胞浸潤能 / 細胞遊走能 / 基質分解酵素 / 癌抑制遺伝子 / 自然免疫 / 獲得免疫 / カテリシジンファミリー / 抗腫瘍効果

Research Abstract

We studied the antimicrobial and antitumor effects of various host defense peptides (HDPs) associates with innate immunity. Additionally, we demonstrated the only member of the cathelicidin family identified in humans, as human cationic antimicrobial protein 18 (hCAP18) regulates signal transduction in epithelial cells and squamous cell carcinoma cells. Our results demonstrated that the bacterial killing of hCAP18 was synergistic with defensin and lactobacilluspeptide, as lactacin. In addition, we show that hCAP18 encoded CAMP gene is tumor suppressive activity in human squamous cell carcinoma.

Research Products

• [Journal Article] Cathelicidins-therapeutic antimicrobial and antitumor host defense peptides for oral diseases. (2010)
  • Author(s): Okumura, K.
  • Journal Title: J. Dent. Res. Science (In press)
  • NAID: 10027850259
  • Peer Reviewed
• [Journal Article] Cathelicidins-therapeutic antimicrobial and antitumor host defense peptides for oral diseases. (2010)
  • Author(s): Okumura, K
  • Journal Title: J.Dent.Res.Science. (In press)
  • NAID: 10027850259
  • Peer Reviewed
• [Journal Article] Ceragenin CSA-13 exhibits antimicrobial activity against cariogenic and periodontopathic bacteria (2009)
  • Author(s): Isogai E, Isogai H, Takahashi K, Okumura K, Savage P B
  • Journal Title: Oral Microbiol Immunol 24 Pages: 170-172
  • Peer Reviewed
• [Journal Article] Antimicrobial activity of three tick defensins and four mammalian cathlicidin-derived synthetic peptides against Lyme disease spirochetes and bacteria isolated from the midgut (2009)
  • Author(s): Isogai, E., Isogai, H., Takahashi, K., Kobayashi-Sakamoto, M., Okumura, K.
  • Journal Title: Exp. Appl. Acrol. 49 Pages: 221-228
  • Peer Reviewed
• [Journal Article] Antimicrobial activity of three tick defensing and four mammalian cathlicidin-derived synthetic peptides against Lyme disease spirochetes and bacteria isolated from the midgut. (2009)
  • Author(s): Isogai, E., Isogai, H., Takahashi, K., Kobayashi-Sakamoto, M., Okumura, K
  • Journal Title: Exp.Appl.Acrol. 49 Pages: 221-228
  • Peer Reviewed
• [Journal Article] Ceragenin CSA-13 exhibits antimicrobial activity against cario genie and periodontopathic bacteria (2009)
  • Author(s): Isogai, E., Isogai, H., Takahashi, K., Okumura, K., Savege P B
  • Journal Title: Oral Microbiol Immunol 24 Pages: 170-172
  • Peer Reviewed
• [Journal Article] Ceragenin CSA-13 exhibits antimicrobial activity against cariogenic and periodontopathic bacteria. (2008)
  • Author(s): Isogai E, Isogai H, Takahashi K, Okumura K. Savage PB.
  • Journal Title: Oral Microbiol Immunol (In press)
  • Peer Reviewed
• [Presentation] ヒト由来抗菌蛋白質hCAP18/LL-37導入癌細胞の浸潤転移形質の低下 (2009)
  • Author(s): 奥村一彦, 平博彦, 磯貝恵美子, 磯貝浩
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Tumor suppression by hCAP18 in oral squamous cell carcinoma cells. (2009)
  • Author(s): Okumura, K., Sawada, N., Taira, H., Isogai, E., Shibata, T., Isogai, H.
  • Organizer: IADR-Continental European Division (CED)
  • Place of Presentation: Gasteig Convention Centre, Munich, Germany
• [Presentation] ヒト抗菌ペプチドhCAP18/LL-37は、上皮間葉移行を介し細胞遊走を制御する (2009)
  • Author(s): 奥村一彦、平博彦、柴田考典
  • Organizer: 第54回(社)日本口腔外科学会総会・学術大会
  • Place of Presentation: 札幌コンベンションセンター(札幌)
• [Presentation] Tumor suppression by hCAP18 in oral squamous cell carcinoma cells. (2009)
  • Author(s): Okumura, K., Sawada, N., Taira, H., Isogai, E., Shibata, T., Isogai, H.
  • Organizer: IADR-Continental European Division (CED),
  • Place of Presentation: Gasteig Convention Centre, Munich, Germany.
• [Presentation] ヒト抗菌ペプチドhCAP18/LL-37は、上皮間葉移行を介し細胞遊走を制御する (2009)
  • Author(s): 奥村一彦、平慱彦、柴田考典
  • Organizer: 第54回(社)日本口腔外科学会総会・学術大会
  • Place of Presentation: 札幌ユンベンションセンー(北海道)
• [Presentation] Antimicrobial activity of tick defensins against Lyme disease spirochetes and bacteria isolated from the midgut. (2008)
  • Author(s): Isogai, H, Isogai, E, Takahashi, K, Kobayashi-Sakamoto, M, Okumura, K
  • Organizer: 11th International conference Lyme borreliosis & other tick-borne diseases
  • Place of Presentation: Irvine, California, USA
• [Presentation] 癌細胞が発現するケモカイン受容体CX3CR1を介した局所浸潤機構の解析-癌原発巣周囲の血管内皮細胞の関与- (2008)
  • Author(s): 奥村一彦, 村岡勝美, 河東秀貴, 細川洋一郎, 磯貝恵美子, 田中真樹, 有末眞, 柴田考典
  • Organizer: 第62回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Antimicrobial activity of tick defensins against Lyme disease spirochetes and bacteria isolated from the midgut. (2008)
  • Author(s): Isogai, H., Isogai, E., Takahashi, K., Kobayashi-Sakamoto, M., Okumura, K
  • Organizer: 11^<th> International conference Lyme borrelios is & other tick-borne diseases
  • Place of Presentation: Irvine, California, USA
• [Presentation] 炎症性サイトカイン, 増殖因子は, 癌細胞のケモカイン受容体発現を増強し浸潤性を促進する (2007)
  • Author(s): 河東秀貴, 奥村一彦, 村岡勝美, 有末眞
  • Organizer: 第61回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 高浸潤性舌扁平上皮癌細胞のFractakineによる浸潤促進機構の解析 (2007)
  • Author(s): 村岡勝美, 奥村一彦, 北所弘行, 河東秀貴, 永易裕樹.有末眞
  • Organizer: 第61回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Expression of hCAP18 is resistant factor against hCAP18-induces apoptosis (2007)
  • Author(s): Okumura, K. Sawada, N, Muraoka, K. Kato, H, Isogai, E, Hosokawa, Y, Shibata, T, Isogai, H.
  • Organizer: 42nd Annual Meeting IADR/CED-ID
  • Place of Presentation: Thessaloniki, Greece
• [Presentation] 炎症性サイトカイン,増殖因子は,癌細胞のケモカイン受容体発現を増強し浸潤性を促進する. (2007)
  • Author(s): 河東 秀貴, 奥村 一彦, 村岡 勝美, 有末 眞
  • Organizer: 第61回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 高浸潤性舌扁平上皮癌細胞のFractakineによる浸潤促進機構の解析. (2007)
  • Author(s): 村岡 勝美, 奥村 一彦, 北所 弘行, 河東 秀貴, 永易 裕樹, 有末 眞
  • Organizer: 第61回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 神戸国際会議場
• [Presentation] ヒト好中球由来hCAPI8/LL-37遺伝子導入細胞による抗菌ペプチド産生と抗菌抗癌効果. (2007)
  • Author(s): 奥村 一彦, 平 博彦, 村岡 勝美, 河東 秀貴, 柴田 考典
  • Organizer: 第61回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Expression of hCAP18 is resistant factor against hCCAPl8-induces apoptosis. (2007)
  • Author(s): K.Okumura, N, Sawada, K.Muraoka, H.Kato, E.Isogai, Y.Hosokawa, T.Shibata, H.Isogai
  • Organizer: 42^<nd> Annual Meeting IADR/CED-ID
  • Place of Presentation: Thessaloniki, Greece