Fibulin upregulated by mechanical strain controls the formation of oxytalan fibers.

• Project/Area Number: 19592391
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Fukuoka Dental College
• Principal Investigator: TSURUGA Eichi Fukuoka Dental College, 歯学部, 准教授 (30295901)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯根膜 / オキシタラン線維 / 弾性系線維 / 細胞外基質 / 線維芽細胞 / 細胞・組織 / 歯学

Research Abstract

Human periodontal ligaments (PDL) contain oxytalan fibers. However, the behavior of oxytalan fibers is unclear. We hypothesized that the fibulin-5 contributes to oxytalan fiber formation under mechanical strain. Fibrillin-1 contains the RGD motif. We subjected PDL fibroblasts to stretching strain. The oxytalan fibers coalesced by stretching. Fibulin-5 colocalizes on oxytalan fibers. Stretching increased fibulin-5 gene expression. The fibulin-5 suppression inhibited the coalescence of oxytalan fibers. The treatment with the integrin αvβ3 antagonist inhibited fibrillin-1 deposition. These results suggest that fibulin-5 may control the formation of oxytalan fibers bundles in PDL.

Research Products

• [Journal Article] Stretching modulates oxytalan fibers in human periodontal ligament cells. (2009)
  • Author(s): Tsuruga E, Nakashima K, Ishikawa H, Yajima T, Sawa Y.
  • Journal Title: J Periodontal Res 44 Pages: 170-174
  • Peer Reviewed
• [Journal Article] Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells. (2009)
  • Author(s): suruga E, Sato A, Ueki T, Nakashima K, Nakatomi Y, Ishikawa H, Yajima T, Sawa Y.
  • Journal Title: Tissue and Cell 41 Pages: 85-89
  • Peer Reviewed
• [Journal Article] Stretching stimulates fibulin-5 expression and controls microfibril bundles in human periodontal ligament cells. (2009)
  • Author(s): Nakashima K, Tsuruga E, Hisanaga Y, Ishikawa H, Sawa Y.
  • Journal Title: J Periodontal Res 44 Pages: 622-627
  • Peer Reviewed
• [Journal Article] Fibulin-5 contributes to microfibril assembly in human periodontal ligament cells. (2009)
  • Author(s): Hisanaga Y, Nakashima K, Tsuruga E, Nakatomi Y, Hatakeyama Y, Ishikawa H, Sawa Y.
  • Journal Title: Acta Histochem Cytochem 42 Pages: 151-157
  • NAID: 130000117893
  • Peer Reviewed
• [Journal Article] Stretching modulates oxytalan fibers in human periodontal ligament cells. (2009)
  • Author(s): 敦賀英知
  • Journal Title: Journal of Periodontal Research 44 Pages: 170-174
  • Peer Reviewed
• [Journal Article] Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells. (2009)
  • Author(s): 敦賀英知
  • Journal Title: Tissue and Cell 41 Pages: 85-89
  • Peer Reviewed
• [Journal Article] Stretching stimulates fibulin-5 expression and controls microfibril bundles in human periodontal ligament cells. (2009)
  • Author(s): 中島一記
  • Journal Title: Journal of Periodontal Research 44 Pages: 622-627
  • Peer Reviewed
• [Presentation] LTBP-2・fibulin-5複合体によるオキシタラン線維形成の制御 (2009)
  • Author(s): 敦賀英知
  • Organizer: 第51回日本歯科基礎医学会
  • Place of Presentation: 新潟コンベンションセンター
  • Year and Date: 2009-09-11
• [Presentation] Integrinαvβ3 regulates microfibril assembly in human periodontal ligament cells. (2008)
  • Author(s): 敦賀英知
  • Organizer: 第50回日本歯科基礎医学会
  • Place of Presentation: TOC有明コンベンションホール
  • Year and Date: 2008-09-23
• [Presentation] Integrin alphavbeta3 regulates microfibril assembly in human periodontal ligament cells. (2008)
  • Author(s): 敦賀英知
  • Organizer: 第50回日本歯科基礎医学会
  • Place of Presentation: TOC有明コンベンシヨンホール
  • Year and Date: 2008-09-23
• [Presentation] TOC有明コンベンションホール3.敦賀英知、Fibulin-5は微細線維上に存在し、微細線維の形成を調節する (2007)
  • Author(s): 敦賀英知
  • Organizer: 第49回歯科基礎医学会学術総会
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2007-08-31
• [Presentation] Fibulin-5は微細線維上に存在し、微細線維の形成を調節する (2007)
  • Author(s): 敦賀 英知
  • Organizer: 第49回歯科基礎医学会学術総会
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2007-08-31