Study for target molecules of poIyphenoIs to evaluate their functions and safety.

• Project/Area Number: 19688007
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Food science
• Research Institution: The University of Tokushima
• Principal Investigator: KAWAI Yoshichika The University of Tokushima, 大学院・ヘルスバイオサエン研究部, 助教 (50380027)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000); Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2008: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2007: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: ポリフェノール / 酸化ストレス / マクロファージ / 動脈硬化症 / 炎症 / βグルクロニダーゼ / オートファジー / 標的分子 / β-グルクロニダーゼ / 疾病予防 / プロテオミクス / 抗酸化物質 / モノクローナル抗体 / 老化 / フラボノイド

Research Abstract

To investigate the molecular mechanism for the functions and safety of polyphenols and to identify their molecular targets, we developed novel monoclonal antibodies that specifically recognize quercetin or epicatechin gallate. Using these antibodies, we have identified macrophage cells in human aorta as the specific target of these polyphenols.

Research Products

• [Journal Article] Effect of quercetin and glucuronide metabolites on the monoamine oxidase-A reaction in mouse brain mitochondria. (2011)
  • Author(s): Yoshino S, et al.
  • Journal Title: Nutrition Volume: (In press)
  • Peer Reviewed
• [Journal Article] Suppressive effect of quercetin on acute stress-induced hypothalamic-pituitary-adrenal axis response in Wistar rats. (2010)
  • Author(s): Kawabata K, et al.
  • Journal Title: Nutr Biochem. Volume: 21 Pages: 374-380
  • Peer Reviewed
• [Journal Article] Different profiles of quercetin metabolites in rat plasma : comparison of two administration methods. (2009)
  • Author(s): Kawai, Y., Saito, S., Nishikawa, T., Ishisaka, A., Murota, K., Terao, J.
  • Journal Title: Biosci. Biotechnol.Biochem. 73 Pages: 517-523
  • NAID: 10027538165
• [Journal Article] Different profiles of quercetin metabolites in rat plasma: comparison of two administration methods. (2009)
  • Author(s): Kawai Y, Saito S, Nishikawa T, Ishisaka A, Murota K, Terao J.
  • Journal Title: Biosci Biotechnol Biochem 73 Pages: 517-23
  • NAID: 10027538165
  • Peer Reviewed
• [Journal Article] Different profiles of quercetin metabolites in rat plasma : comparison of two administration methods (2009)
  • Author(s): Kawai Y, et al.
  • Journal Title: Bioscience Biotechnology and Biochemistry 73 Pages: 517-526
  • NAID: 10027538165
  • Peer Reviewed
• [Journal Article] (-)-Epicatechin gallate accumulates in foamy macrophages in human atherosclerotic aorta : implication in the anti-atherosclerotic actions of tea catechins. (2008)
  • Author(s): Kawai, Y., Tanaka, H., Murota, K., Naito, M., Terao J.
  • Journal Title: Biochem.Biophys.Res. 374 Pages: 527-532
• [Journal Article] Flavonoids as substrates and inhibitors of myeloperoxidase: molecular actions of aglycone and metabolites. (2008)
  • Author(s): Shiba, Y., Kinoshita, T., Chuman, H., Taketani, Y., Takeda, E., Kato, Y., Naito, M., Kawabata, K., Ishisaka, A., Terao, J., Kawai, Y.
  • Journal Title: Chem.Res. Toxicol. 21 Pages: 1600-1609
• [Journal Article] Macrophage as a target of quercetin glucuronides in human atherosclerotic arteries: implication in the anti-atherosclerotic mechanism of dietary flavonoids. (2008)
  • Author(s): Kawai, Y., Nishikawa, T., Shiba, Y., Saito, S., Murota, K., Shibata, N., Kobayashi, M., Kanayama, M., Uchida, K., Terao, J.
  • Journal Title: J.Biol.Chem. 283 Pages: 9424-9434
• [Journal Article] Galloylated catechins as potent inhibitors of hypochlorous acid-induced DNA damage. (2008)
  • Author(s): Kawai, Y., Matsui, Y., Kondo, H., Morinaga, H., Uchida, K., Miyoshi, N., Nakamura, Y., Osawa, T.
  • Journal Title: Chem.Res. Toxicol. 21 Pages: 1407-1414
• [Journal Article] Immunochemical detection of flavonoid glycosides: development, specificity, and application of novel monoclonal antibodies. (2008)
  • Author(s): Kawai, Y., Ishisaka, A., Saito, S., Uchida, K., Shibata, N., Kobayashi, M., Fukuchi, Y., Naito, M., Terao, J.
  • Journal Title: Arch.Biochem.Biophys. 476 Pages: 124-132
• [Journal Article] (-)-Epicatechin gallate accumulates in foamy macrophages in human atherosclerotic aorta: implication in the anti-atherosclerotic actions of tea catechins. (2008)
  • Author(s): Kawai Y, Tanaka H, Murota K, Naito M, Terao J
  • Journal Title: Biochem Biophys Res Commun 374 Pages: 527-32
  • Peer Reviewed
• [Journal Article] Flavonoids as substrates and inhibitors of myeloperoxidase: molecular actions of aglycone and metabolites. (2008)
  • Author(s): Shiba Y, Kinoshita T, Chuman H, Taketani Y, Takeda E, Kato Y, Naito M, Kawabata K, Ishisaka A, Terao J, Kawai Y.
  • Journal Title: Chem Res Toxicol 21 Pages: 1600-9
  • Peer Reviewed
• [Journal Article] Immunochemical detection of flavonoid glycosides: development, specificity, and application of novel monoclonal antibodies. (2008)
  • Author(s): Kawai Y, Ishisaka A, Saito S, Uchida K, Shibata N, Kobayashi M, Fukuchi Y, Naito M, Terao J.
  • Journal Title: Arch Biochem Biophys 476 Pages: 124-32
  • Peer Reviewed
• [Journal Article] Macrophage as a target of quercetin glucuronides in human atherosclerotic arteries: implication in the anti-atherosclerotic mechanism of dietary flavonoids. (2008)
  • Author(s): Kawai Y, Nishikawa T, Shiba Y, Saito S, Murota K, Shibata N, Kobayashi M, Kanayama M, Uchida K, Terao J.
  • Journal Title: J Biol Chem 283 Pages: 9424-34
  • Peer Reviewed
• [Journal Article] Immunochemical detection of flavonoid glycosides : development, specificity, and application of novel monoclonal antibodies (2008)
  • Author(s): Kawai, Y., et.al.
  • Journal Title: Archives of Biochemistry and Biophysics 476 Pages: 124-132
  • Peer Reviewed
• [Journal Article] (-)-Epicatechin gallate accumulates in foamy macrophages in human atherosclerotic aorta : implication in the anti-atherosclerotic actions of tea catechins (2008)
  • Author(s): Kawai, Y., et.al.
  • Journal Title: Biochemical and Biophysical Research Communications 374 Pages: 527-532
  • Peer Reviewed
• [Journal Article] Flavonoids as substrates and inhibitors of myeloperoxidase : molecular actions of aglycone and metabolites (2008)
  • Author(s): Shiba, Y., et.al.
  • Journal Title: Chemical Research in Toxicology 21 Pages: 1600-1609
  • Peer Reviewed
• [Journal Article] Macrophage as a target of quercetin glucuronides in human atherosclerotic arteries:implication in the anti-atherosclerotic mechanism of dietary flavonoids (2008)
  • Author(s): Kawai, Y., et. al.
  • Journal Title: The Journal of Biological Chemistry 283 Pages: 9424-9434
  • Peer Reviewed
• [Presentation] 炎症部位における食品由来ポリフェノールの選択的作用機構 (2010)
  • Author(s): 河合慶親
  • Organizer: 第27回臨床フリーラジカル会議
  • Place of Presentation: 亀岡
  • Year and Date: 2010-12-03
• [Presentation] 炎症部位における食品由来ポリフェノールの選択的作用機構 (2010)
  • Author(s): 河合慶親
  • Organizer: 第27回臨床フリーラジカル会議
  • Place of Presentation: 京都府亀岡市
  • Year and Date: 2010-12-03
• [Presentation] 機能性ポリフェノールの標的部位と作用機構:抗ポリフェノール抗体の開発から見えてきたこと (2010)
  • Author(s): 河合慶親、寺尾純二
  • Organizer: 日本農芸化学会20 09年度大会シンポジウム
  • Place of Presentation: 福岡
  • Year and Date: 2010-03-28
• [Presentation] 抗酸化食品因子の生体内標的部位と酸化ストレス制御機構に関する研究 (2010)
  • Author(s): 河合慶親
  • Organizer: 日本農芸化学会中四国支部第26回講演会
  • Place of Presentation: 松山
  • Year and Date: 2010-01-23
• [Presentation] 抗酸化食品因子の生体内標的部位と酸化ストレス制御機構に関する研究 (2010)
  • Author(s): 河合慶親
  • Organizer: 日本農芸化学会中四国支部第26回講演
  • Place of Presentation: 松山
  • Year and Date: 2010-01-23
• [Presentation] 抗酸化食品因子の生体内標的部位と酸化ストルス制御機構に関する研究 (2010)
  • Author(s): 河合慶親
  • Organizer: 日本農芸化学会中四国支部第26回講演会
  • Place of Presentation: 愛媛大学(松山)
  • Year and Date: 2010-01-23
• [Presentation] 機能性ポリフェノールの標的部位と作用機構:抗ポリフェノール抗体の開発から見えてきたこと (2009)
  • Author(s): 河合慶親
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] 機能性ポリフェノールの標的部位と作用機構 : 抗ポリフェノール抗体の開発から見えてきたこと (2009)
  • Author(s): 河合慶親、寺尾純二
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡国際会議場(博多)
  • Year and Date: 2009-03-28
• [Presentation] Immunochemical characterization of flavonoid metabolites in aorta: implication in the anti-atherosclerotic effect (2008)
  • Author(s): 河合慶親
  • Organizer: 3 rd International Conference on Polyphenol and Health (ICPH)
  • Place of Presentation: 京都
  • Year and Date: 2008-11-26
• [Presentation] 脱抱合を介したケルセチン抱合体の活性発現機構 (2008)
  • Author(s): 河合慶親
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-27
• [Presentation] 脱抱合を介したケルセチン抱合体の活性発現機構 (2008)
  • Author(s): 河合慶親ら
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名城大学天白キャンパス(名古屋)
  • Year and Date: 2008-03-27
• [Presentation] Immunochemical characterization of dietary f-avonoid metabolites in aorta: Implication in the anti-atherosclerosis effects (2007)
  • Author(s): Yoshichika Kawai
  • Organizer: 3rd International Conference on Polyphenols and Health
  • Place of Presentation: ICPH シンポジウム講演
  • Year and Date: 2007-11-26
• [Presentation] Immunochemical characterization of flavonoid metabolites in aorta:implication in the anti-atherosclerotic effect (2007)
  • Author(s): 河合慶親
  • Organizer: 3 rd International Conference on Polyphenol and Health(ICPH)
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 2007-11-26
• [Presentation] ポリフェノールによる抗動脈 硬化作用機構の新展開 (2007)
  • Author(s): 河合慶親
  • Organizer: 日本栄養・食糧 学会中四国支部大会
  • Place of Presentation: 広島 シンポジウム講演
  • Year and Date: 2007-11-17
• [Book] Anti-atherosclerotic Effects of Dietary Flavonoids: Insight into their Molecular Action Mechanism at the Target Site、Recent Advances in Polyphenol Research (2010)
  • Author(s): Junji Terao, Kaeko Murota, Yoshichika Kawai
  • Publisher: WILEY-BLACKWELL
• [Book] ケルセチンの生体内標的部位と作用機構:抗ポリフェノール抗体の開発と応用から見えてきたものとは? (2009)
  • Author(s): 河合慶親、寺尾純二
  • Publisher: 学会出版センター化学と生物(社団法人日本農芸化学会)