Functional analysis and comprehensive isolation of meiosis-specific genes in Schizosaccharomyces pombe.

• Project/Area Number: 19710164
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 基礎ゲノム科学
• Research Institution: Osaka University
• Principal Investigator: OKUZAKI Daisuke Osaka University, 微生物病研究所, 助教 (00346131)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,940,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥540,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: 分裂酵母 / 減数分裂 / 生殖・分化 / 細胞周期 / リン酸化 / 配偶子形成 / RNA / 前胞子膜形成 / オルガネラ / RNA結合タンパク / 遺伝子発現 / コイルドコイル / 組換え / 染色体 / 環境応答

Research Abstract

Germ cell research in humans and higher organisms, but is essential and urgent task facing the ethical and technical issues. To overcome this problem continues to study more than ten years ago with a model organism for the study of meiosis in fission yeast reproduction. Isolation and functional analysis performed comprehensive transcription of genes specifically induced by meiosis-based comprehensive analysis of genome information and proprietary technology that also exist in mammalian genes aimed at understanding the mechanism of mammalian reproduction identified by analyzing a large number.

Research Products

• [Journal Article] Upregulation of insulin-like growth factor binding protein 3 in astrocytes of transgenic mice that express borna disease virus phosphoprotein. (2011)
  • Author(s): Honda T, Fujino K, Okuzaki D, Ohtaki N, Matsumoto Y, Horie M, Daito T, Itoh M, Tomonaga K.
  • Journal Title: J Virol. 85(9) Pages: 4567-4571
  • Peer Reviewed
• [Journal Article] MicroRNA-mediated downregulation of mTOR/FGFR3 controls tumor growth induced by Src-related oncogenic pathways.Oncogene. (2011)
  • Author(s): Oneyama C, Ikeda J, Okuzaki D, Suzuki K, Kanou T, Shintani Y, Morii E, Okumura M, Aozasa K, Okada M.
  • Journal Title: 2011 Oncogene advance online publication 63
  • Peer Reviewed
• [Journal Article] Micro RNA-mediated downregulation of mTOR/FGFR3 controls tumor growth induced by Src-related oncogenic pathways. (2011)
  • Author(s): Oneyama C, Ikeda J, Okuzaki D, Suzuki K, Kanou T, Shintani Y, Morii E, Okumura M, Aozasa K, Okada M.
  • Journal Title: Oncogene Volume: (印刷中 未定)
  • Peer Reviewed
• [Journal Article] Upregulation of insulin-like growth factor binding protein 3 in astrocytes of transgenic mice expressing Borna disease virus phosphoprotein. (2011)
  • Author(s): Honda T, Fujino K, Okuzaki D, Ohtaki N, Matsumoto Y, Horie M, Daito T, Itoh M, Tomonaga K.
  • Journal Title: J.Virol. Volume: (印刷中 未定)
  • Peer Reviewed
• [Journal Article] The Mek1 phosphorylation cascade plays a role in meiotic recombination of Schizosaccharomyces pombe. (2010)
  • Author(s): Tougan T, Kasama T, Ohtaka A, Okuzaki D, Saito TT, Russell P, Nojima H.
  • Journal Title: Cell Cycle. 1;9(23) Pages: 4688-4702
  • Peer Reviewed
• [Journal Article] GenopalTM : a novel hollow fibre array for focused microarray analysis (2010)
  • Author(s): Okuzaki D, Fukushima T, Tougan T, Ishii T, Kobayashi S, Yoshizaki K, Akita T, Nojima H.
  • Journal Title: DNA Res. 17(6) Pages: 369-379
  • Peer Reviewed
• [Journal Article] Nucleoredoxin sustains Wnt/β-catenin signaling by retaining a pool of inactive dishevelled protein. (2010)
  • Author(s): Funato Y, Terabayashi T, Sakamoto R, Okuzaki D, Ichise H, Nojima H, Yoshida N, Miki H.
  • Journal Title: Curr Biol. 9;20(21) Pages: 1945-1952
  • Peer Reviewed
• [Journal Article] Spo5 phosphorylation is essential for its own timely degradation and for successful meiosis in Schizosaccharomyces pombe. (2010)
  • Author(s): Okuzaki D, Kasama T, Hirata A, Ohtaka A, Kakegawa R, Nojima H.
  • Journal Title: Cell Cycle. 15;9(18) Pages: 3751-3760
• [Journal Article] Mug28, a meiosis-specific protein of Schizosaccharomyces pombe, regulates spore wall formation. (2010)
  • Author(s): Shigehisa A, Okuzaki D, Kasama T, Tohda H, Hirata A, Nojima H.
  • Journal Title: Mol Biol Cell. 15;21(12) Pages: 1955-1967
  • Peer Reviewed
• [Journal Article] Microarray analysis of tonsils in immunoglobulin A nephropathy patients. (2010)
  • Author(s): Iio K, Nagasawa Y, Iwatani H, Yamamoto R, Horii A, Okuzaki D, Furumatsu Y, Inohara H, Nojima H, Imai E, Isaka Y, Rakugi H.
  • Journal Title: Biochem Biophys Res Commun. 19;393(4)
  • Peer Reviewed
• [Journal Article] The Mek1 phosphorylation cascade plays a role in meiotic recombination of Schizosaccharomyces pombe. (2010)
  • Author(s): Tougan T, Kasama T, Ohtaka A, Okuzaki D, Saito TT, Russell P, Nojima H.
  • Journal Title: Cell Cycle. Volume: 9(23) Pages: 4688-4702
  • Peer Reviewed
• [Journal Article] Genopal^<TM> : a novel hollow fibre array for focused microarray analysis. (2010)
  • Author(s): Okuzaki D, Fukushima T, Tougan T, Ishii T, Kobayashi S, Yoshizaki K, Akita T, Nojima H.
  • Journal Title: DNA Res. Volume: 17(6) Pages: 369-379
  • Peer Reviewed
• [Journal Article] Nucleoredoxin sustains Wnt/β-catenin signaling by retaining a pool of inactive dishevelled protein. (2010)
  • Author(s): Funato Y, Terabayashi T, Sakamoto R, Okuzaki D, Ichise H, Nojima H, Yoshida N, Miki H.
  • Journal Title: Curr Biol. Volume: 20(21) Pages: 1945-1952
  • Peer Reviewed
• [Journal Article] Spo5 phosphorylation is essential for its own timely degradation and for successful meiosis in Schizosaccharomyces pombe. (2010)
  • Author(s): Okuzaki D, Kasama T, Hirata A, Ohtaka A, Kakegawa R, Nojima H.
  • Journal Title: Cell Cycle. Volume: 9(18) Pages: 3751-3760
  • Peer Reviewed
• [Journal Article] Mug28, a meiosis-specific protein of Schizosaccharomyces pombe, regulates spore wall formation. (2010)
  • Author(s): Shigehisa A, Okuzaki D, Kasama T, Tohda H, Hirata A, Nojima H.
  • Journal Title: Mol Biol Cell. Volume: 21(12) Pages: 1955-1967
  • Peer Reviewed
• [Journal Article] Microarray analysis of tonsils in immunoglobulin A nephropathy patients. (2010)
  • Author(s): Iio K, Nagasawa Y, Iwatani H, Yamamoto R, Horii A, Okuzaki D, Furumatsu Y, Inohara H, Nojima H, Imai E, Isaka Y, Rakugi H.
  • Journal Title: Biochem Biophys Res Commun. Volume: 393(4) Pages: 565-570
  • Peer Reviewed
• [Journal Article] Mug28, a meiosis-specific protein of Schizosaccharomyces pombe, regulates spore wall formation. (2010)
  • Author(s): Okuzaki D, Shigehisa A, Kasama T, Tohda H, Hi rata A, Nojima H.
  • Journal Title: Molecular Biology of the Cell (in press)
  • Peer Reviewed
• [Journal Article] Mug28, a meiosis-specific protein of Schizosaccharomyces pombe, regulates spore wall formation (2010)
  • Author(s): D.Okuzaki, et al.
  • Journal Title: Molecular Biology of the Cell (未定)(in press)
  • Peer Reviewed
• [Journal Article] Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification. (2008)
  • Author(s): Tougan T, Okuzaki D, Nojima H.
  • Journal Title: Nucleic Acids Res. 36(15) Pages: 92-92
  • Peer Reviewed
• [Journal Article] Expression profiling of PBMC-based diagnostic gene markers isolated from vasculitis patients. (2008)
  • Author(s): Kobayashi S, Ito A, Okuzaki D, Onda H, Yabuta N, Nagamori I, Suzuki K, Hashimoto H, Nojima H.
  • Journal Title: DNA Res. 15(4) Pages: 253-265
  • Peer Reviewed
• [Journal Article] Mug27 is a meiosis-specific protein kinase that functions in fission yeast meiosis II and sporulation. (2008)
  • Author(s): Ohtaka A, Okuzaki D, Nojima H.
  • Journal Title: J Cell Sci. 1;121(Pt 9) Pages: 1547-1558
  • Peer Reviewed
• [Journal Article] Focused microarray analysis of peripheral mononuclear blood cells from Churg-Strauss syndrome patients. (2008)
  • Author(s): Tougan T, Onda H, Okuzaki D, Kobayashi S, Hashimoto H, Nojima H.
  • Journal Title: DNA Res. 30;15(2) Pages: 103-114
  • Peer Reviewed
• [Journal Article] Mug27/Ppk35 is a meiosis-specific protein kinase that functions in fission yeast meiosis II and sporulation. (2008)
  • Author(s): Ohtaka A, Okuzaki D, Noj ima H
  • Journal Title: J. Cell Sci. vol.121,No.9 Pages: 1547-1558
  • Peer Reviewed
• [Journal Article] Mug27 is a meiosis-specific protein kinase that functions in fission yeast meiosis II and sporulation (2008)
  • Author(s): A. Ohtaka, et.al.
  • Journal Title: Journal of Cell Science 121 Pages: 1547-58
  • Peer Reviewed
• [Journal Article] Focused Microattay Analysis of Peripheral Mononuclear Blood Cells from Churg-Strauss Syndrome Patients. (2008)
  • Author(s): T. Tougan, et. al.
  • Journal Title: DNA Research 7 Pages: 1-18
  • Peer Reviewed
• [Journal Article] Lats2 is an essential mitotic regulator required for the coordination of cell division. (2007)
  • Author(s): Yabuta N, Okada N, Ito A, Hosomi T, Nishihara S, Sasayama Y, Fujimori A, Okuzaki D, Zhao H, Ikawa M, Okabe M, Nojima H.
  • Journal Title: J Biol Chem. 29;282(26) Pages: 19259-19271
  • Peer Reviewed
• [Journal Article] Mcp4, a meiotic coiled-coil protein, plays a role in F-actin positioning during Schizosaccharomyces pombe meiosis. (2007)
  • Author(s): Ohtaka A, Okuzaki D, Saito TT, Nojima H.
  • Journal Title: Eukaryot Cell. 6(6) Pages: 971-983
  • Peer Reviewed
• [Journal Article] Meiosis specific coiled-coil proteins in Shizosaccharomyces pombe. (2007)
  • Author(s): Ohtaka A, Saito TT, Okuzaki D, Nojima H.
  • Journal Title: Cell Div. 18;2 Pages: 14-14
  • Peer Reviewed
• [Journal Article] Meiosis specific coiled-coil proteins in Shizosaccharomyces pombe. (2007)
  • Author(s): Ohtaka A, Saito TT, Okuzaki D, Noj ima H
  • Journal Title: Cell Div. Vol.2,No.14
  • Peer Reviewed
• [Journal Article] Lats2 is an essential mitotic regulator required for the coordination of cell division. (2007)
  • Author(s): Yabuta N, Okada N, Ito A, Hosomi T, Nishihara S, Sasayama Y, Fuj irnori A, Okuzaki D, Zhao H, Ikawa M, Okabe M, Noj ima H
  • Journal Title: J Biol Chem vol.282,No.26 Pages: 19259-19271
  • Peer Reviewed
• [Journal Article] Mcp4, a meiotic coiled-coil protein, plays a role in F-act in positioning during Schizosaccharomlyces pombe meiosis. (2007)
  • Author(s): Ohtaka A, Okuzaki D, Saito TT, Noj ima H
  • Journal Title: Eukaryot Cell. vol.6,No.6 Pages: 971-983
  • Peer Reviewed
• [Journal Article] Mcp4, a meiotic coiled-coil proteim, plays a role in F-actim positioning during Schizosaccharomyces pombe meioshis. (2007)
  • Author(s): A. Ohtaka, et. al.
  • Journal Title: Eukaryotic Cell 6 Pages: 971-983
  • Peer Reviewed
• [Journal Article] Lats2 in an essential mitotic regulator required for the coordination of cell division. (2007)
  • Author(s): N. Yabuta, et. al.
  • Journal Title: Journal of Biological Chemistry 282 Pages: 19259-19271
  • Peer Reviewed
• [Journal Article] Meiosis specific coiled-coil proteins in Schizosaccharomyces pombe. (2007)
  • Author(s): A. Ohtaka, et. al.
  • Journal Title: Cell Division 2 Pages: 1-8
  • Peer Reviewed
• [Presentation] Spo5 phosphorylation is essential for its own timely degradation and for successful meiosis in Schizosaccharomyces pombe (2010)
  • Author(s): 奥崎大介
  • Organizer: 第33回日本分子生物学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] Spo5 phosphorylation is essential for its own timely degradation and for successful meiosis in Schizosaccharomyces pombe. (2010)
  • Author(s): 奥崎大介
  • Organizer: 第33回日本分子生物学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] A novel Diagnostic markers for Takayasu Arteritis obtained by GenopalTM focused microarray based on Spearman rank correlation (2010)
  • Author(s): 奥崎大介
  • Organizer: 第33回日本分子生物学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] A novel Diagnostic markers for Takayasu Arteritis obtained by GenopalTM focused microarray based on Spearman rank correlation. (2010)
  • Author(s): 奥崎大介
  • Organizer: 第33回日本分子生物学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] 分裂酵母RNA結合タンパク質Mug28による配偶子形成の制御機構 (2010)
  • Author(s): 奥崎大介
  • Organizer: 第43回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: 奈良
  • Year and Date: 2010-09-10
• [Presentation] A novel Diagnostic markers for Takayasu Arteritis obtained by Genopal focused microarray based on Spearman rank correlation (2010)
  • Author(s): 奥崎大介
  • Organizer: 第10回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2010-09-08
• [Presentation] A novel Diagnostic markers for Takayasu Arteritis obtained by Genopal focused microarray based on Spearman rank correlation. (2010)
  • Author(s): 奥崎大介
  • Organizer: 第10回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2010-09-08
• [Presentation] 細胞生物学者の視点でみるマイクロアレイと次世代シーケンス (2010)
  • Author(s): 奥崎大介
  • Organizer: 第62回日本細胞生物学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-05-19
• [Presentation] 細胞生物学者の視点でみるマイクロアレイと次世代シーケンス (2010)
  • Author(s): 奥崎大介
  • Organizer: 第62回日本細胞生物学会
  • Place of Presentation: 大阪国際会議場(招待講演)
  • Year and Date: 2010-05-19
• [Presentation] MUG28, A MEIOSIS-SPECIFIC PROTEIN OF SCHIZOSACCHAROMYCES POMBE, REGULATES SPORE WALL FORMATION (2009)
  • Author(s): 奥崎大介
  • Organizer: The 5th International Fission Yeast Meeting
  • Place of Presentation: 国立オリンピック記念青少年総合センター(東京都)
  • Year and Date: 2009-10-29
• [Presentation] DETECTシステムの開発 (2009)
  • Author(s): 奥崎大介
  • Organizer: 第82回生化学会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2009-10-21
• [Presentation] GenopalTM ; a novel device for focused microarray equipped with hollow fiber array (2009)
  • Author(s): 奥崎大介
  • Organizer: 第9回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2009-09-10
• [Presentation] Genopal^<TM> ; a novel device for focused microarray equipped with hollow fiber array (2009)
  • Author(s): 奥崎大介
  • Organizer: 第9回あわじしま感染症・免疫フオーラム
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2009-09-10
• [Presentation] 分裂酵母の減数分裂特異的リン酸化酵素 : Mug27/Slk1の機能解析 (2008)
  • Author(s): 大高彩美
  • Organizer: Biochemistry And Molecular biology 2008
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] Fission yeast Arp1 facilitates meiotic nuclear oscillation via Dhc1 and Mcp5 (2008)
  • Author(s): 奥崎大介
  • Organizer: The 2008 Yeast Genetics and Molecular Biology Meeting
  • Place of Presentation: カナダ・トロント大学
  • Year and Date: 2008-07-23
• [Presentation] Fission yeast Arp1 facilitates meiotic nuclear oscillation via Dhc1 and Mcp5. (2008)
  • Author(s): 奥崎大介
  • Organizer: The 2008 Yeast Genetics and Molecular Biology Meeting
  • Place of Presentation: カナダ・トロント大学
  • Year and Date: 2008-07-23
• [Presentation] Fission yeast Arpl facilitates meiotic nuclear oscillation via Dhc1 and Mcp5 (2008)
  • Author(s): 奥崎大介
  • Organizer: The 2008 Yeast Genetics and Molecular Biology Meeting
  • Place of Presentation: カナダ・トロント大学
  • Year and Date: 2008-07-23
• [Presentation] The fission yeast Mug27/ppk35, a mammalian Lats2 homologue, is a meiosis-specific protein kinase that functions in meiosis II and sporulation (2008)
  • Author(s): 大高彩美
  • Organizer: The 2008 Yeast Genetics and Molecular Biology Meeting
  • Place of Presentation: カナダ・トロント大学
  • Year and Date: 2008-07-23
• [Presentation] 分裂酵母の減数分裂特異的カイネース:Mug27/Lts1の機能解析 (2007)
  • Author(s): 奥崎大介
  • Organizer: 第30回日本分子生物学会年会、BMB2007
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] 分裂酵母の減数分裂特異的カイネース:Mug27/Lts1の機能解析 (2007)
  • Author(s): 奥崎大介
  • Organizer: BMB2007(第30回日本分子生物学会年会)
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] 分裂酵母の減数分裂期特異的コイルドコイルタンパク質:Mcp4の機能解析 (2007)
  • Author(s): 奥崎大介
  • Organizer: 第40回酵母遺伝学フォーラム
  • Place of Presentation: 大阪大学コンベンションセンター
• [Presentation] 分裂酵母の減数分裂期特異的コイルドコイルタンパク質:Mcp4の機能解析 (2007)
  • Author(s): 奥崎大介
  • Organizer: 第40回 酵母遺伝学フォーラム
  • Place of Presentation: 大阪大学コンベンションセンター
• [Book] 月刊バイオインダストリー バイオクラスター 新産業創出と産学連携の新たな挑戦 (内5ページ) (2009)
  • Author(s): 奥崎大介
  • Total Pages: 86
  • Publisher: (株)シーエムシー出版
• [Remarks] ホームページ等
  • URL: http://www.biken.osaka-u.ac.jp/lab/molgenet/
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/DNA_chip/
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/lab/molgenet/
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/lab/molgenet/
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/lab/molgenet/
• [Patent(Industrial Property Rights)] 白血球タンパク質の回収方法および回収装置 (2009)
  • Inventor(s): 野島博、藪田紀一、奥崎大介
  • Industrial Property Rights Holder: 大阪大学
  • Filing Date: 2009-10-26