Role of growth factors in the sexual differentiation in medaka

• Project/Area Number: 19770051
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphology/Structure
• Research Institution: Kumamoto University
• Principal Investigator: KITANO Takeshi Kumamoto University, 大学院・自然科学研究科, 准教授 (40336219)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: メダカ / 性分化 / 生殖細胞

Research Abstract

メダカ性分化において、増殖因子であるMIS(Mullerian inhibiting substance)の生理的機能を明らかにするため、アンチセンスオリゴを用いたMIS及びMIS受容体の機能阻害実験を行った。その結果、MISまたはMIS受容体の機能阻害により、雌雄の生殖細胞の増殖が完全に抑制された。このことから、MISは、メダカ性分化時期において、雌雄の生殖細胞の増殖に不可欠な因子であることが明らかとなった。

Research Products

• [Journal Article] Mullerian inhibiting substance is required for germ cell proliferation during early gonadal differentiation in medaka (Oryzias latipies) (2008)
  • Author(s): Shiraishi E., Yoshinaga N., Miura T., Yokoi H., Wakamatsu Y., Abe S. and Kitano T.
  • Journal Title: Endocrinology 149 Pages: 1813-1819
  • Peer Reviewed
• [Journal Article] Cyp11b1 is induced in the murine gonad by luteinizing hormone/human chorionic gonadotropin and involved in the production of 11-ketotestosterone, a major fish androgen ; conservation and evolution of androgen metabolic pathway. (2008)
  • Author(s): Yazawa T., Uesaka M., Inaoka Y., Mizutani T., Sekiguchi T., Kajitani T., Kitano T., Umezawa A. and Miyamoto K.
  • Journal Title: Endocrinology 149 Pages: 1786-1792
  • Peer Reviewed
• [Journal Article] Mullerian inhibiting substance is required for germ cell proliferation during early gonadal differentiation in medaka (2008)
  • Author(s): Shiraishi E.
  • Journal Title: Endocrinology 149(Oryzias latipies) Pages: 1813-1819
  • Peer Reviewed
• [Journal Article] Cypllb1 is induced in the murine gonad by luteinizing hormone/ human chorionic gonadotropin and involved in the production of 11-ketotestosterone, a major fish androgen ; conservation and evolution of androgen metabolic nathway (2008)
  • Author(s): Yazawa T.
  • Journal Title: Endocrinology 149 Pages: 1786-1792
  • Peer Reviewed
• [Journal Article] Tributyltin-binding protein type 1 has a distinctive lipocalin-like structure and is involved in the excretion of tributyltin in Japanese flounder, Paralichthys olivaceus (2008)
  • Author(s): Satone H.
  • Journal Title: Aquatic Toxicology 90 Pages: 229-238
  • Peer Reviewed
• [Journal Article] The mechanism of transcriptional regulation of Mullerian inhibiting substance in Japanese flounder (2008)
  • Author(s): Shirozu T.
  • Journal Title: Cybium 32(sunpl)(Paralichthvs olivaceus) Pages: 90-91
  • Peer Reviewed
• [Journal Article] The mechanism of transcriptional regulation of P450 aromatase gene by. Fox12 in Japanese flounder (2008)
  • Author(s): Yamaguchi T.
  • Journal Title: Cybium 32 (suppl)(Paralichthys olivaceus) Pages: 74-76
  • Peer Reviewed
• [Journal Article] Strain difference of cadmium accumulation by liver slices of inbred Wistar-Imamichi and Fischer 344 rats. (2008)
  • Author(s): Shimada H.
  • Journal Title: Toxicology in Vitro 22 Pages: 338-343
• [Journal Article] Size-selective junctional barrier and Ca2+independent cell adhesion in the testis of Cynops pyrrhogaster: expression and function of occludin. (2008)
  • Author(s): Jin Y.
  • Journal Title: Molecular Reproduction and Development 75 Pages: 202-216
• [Journal Article] Fish Reproduction (Involvement of endocrine and environmental factors (2008)
  • Author(s): Kitano T.
  • Journal Title: Fish Reproduction (Involvement of endocrine and environmental factors in gonadal sex differentiation in gonochoristic fish.), Science Publishers Pages: 421-434
• [Journal Article] Follicle-stimulating hormone signaling and Fox12 are involved in transcriptional regulation of aromatase gene during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus. (2007)
  • Author(s): Yamaguchi T., Yamaguchi S., Hirai T. and Kitano T.
  • Journal Title: Biochem. Biophys. Res. Commun 359 Pages: 935-940
  • Peer Reviewed
• [Journal Article] Tamoxifen induces masculinization of genetic females and regulates P450 aromatase and Mullerian inhibiting substance mRNA expression in Japanese flounder (Paralichthys olivaceus). (2007)
  • Author(s): Kitano T., Yoshinaga N., Shiraishi E., Koyanagi T. and Abe S.
  • Journal Title: Mol. Reprod. Dev 74 Pages: 1171-1177
  • Peer Reviewed
• [Journal Article] Tamoxifen induces masculinization of genetic females and regulates P450 aromatase and Mullerian inhibiting substance mRNA expression in Japanese flounder(Paralichthys olivaceus). (2007)
  • Author(s): Kitano T.
  • Journal Title: Molecular Reproduction and Development 74 Pages: 1171-1177
• [Journal Article] Follicle-Stimulating hormone signaling and Foxl2 are involved in transcriptional regulation of aromatase gene during gonadal sexdifferentiation in Japanese flounder, Paralichthys olivaceus. (2007)
  • Author(s): Yamaguchi T.
  • Journal Title: Biochemical and Biophysical Research Communications 359 Pages: 935-940
• [Journal Article] Purification and characterization of tributyltin binding protein type 2 from plasma of Japanese flounder. Paralichthys olivaceus. (2007)
  • Author(s): Oba Y.
  • Journal Title: Journal of Biochemistry 142 Pages: 229-238
• [Journal Article] Tributlyltin causes abnormal development in embryo of medaka. Oryzias latipes. (2007)
  • Author(s): Hano T.
  • Journal Title: Chemosphere 69 Pages: 927-933
• [Presentation] Toxicogenomics and sexual differentiation in fish (2008)
  • Author(s): Kitano T (Invited)
  • Organizer: 4th International Conference on Toxicogenomics
  • Place of Presentation: Incheon, Korea
• [Presentation] The mechanism of transcriptional regulation of P450 aromatase gene by Fox12 in Japanese flounder(Paralichthys olivaceus). (2007)
  • Author(s): Yamaguchi T.
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: st Malo, France
  • Year and Date: 2007-06-05
• [Presentation] Role of Mullerian inhibiting substance on gonadal sex differentiation in medaka (Oryzias latipes) (2007)
  • Author(s): Shiraishi E., Miura T., Abe S. and Kitano T.
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: St Malo, France
• [Presentation] The mechanism of transcriptional regulation of Mullerian inhibiting substance in Japanese flounder (Paralichthys olivaceus) (2007)
  • Author(s): Shirozu, T., Shiraishi E., Yoshinaga N., Kanamori A., Kubo Y., Hori H. and Kitano T.
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: St Malo, France
• [Presentation] The mechanism of transcriptional regulation of P450 aromatase gene by Fox12 in Japanese flounder (Paralichthys olivaceus) (2007)
  • Author(s): Yamaguchi T. and Kitano T.
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: StMalo, France
• [Book] Fish Reproduction (Involvement of endocrine and environmental factors in gonadal sex differentiation in gonochoristic fish.) (2008)
  • Author(s): Kitano T.
  • Publisher: Science Publishers
• [Book] Fish Reproduction (2007)
  • Author(s): Kitano T. and Abe S.-I.
  • Publisher: Involvement of endocrine and environmental factors in gonadal sex differentiation in fish