Studies on metal-containing bio-macromolecules by using anomalous solution x-ray scattering techniques

• Project/Area Number: 19770139
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Nagoya University (2009); The Institute of Physical and Chemical Research (2007-2008)
• Principal Investigator: AKIYAMA Shuji The Institute of Physical and Chemical Research, 大学院・理学研究科, 講師 (50391842)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,880,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 溶液散乱 / X線 / 小角散乱 / 異常分散 / 分子量 / タンパク質 / 標準試料 / セレノメチオニン / 吸収端

Research Abstract

Anomalous x-ray solution scattering has been expected to serve as one of the powerful techniques to evaluate distributions of anomalous atoms within bio-macromolecules under physiological solution conditions. In order to apply this method to iron-containing proteins, a survey of optimal measurement conditions near K-edge of iron atom was conducted by using standard protein samples.

Research Products

• [Journal Article] Quality control of protein standards for molecular mass determinations by small-angle X-ray scattering (2010)
  • Author(s): Akiyama S.
  • Journal Title: J. Appl. Cryst. 43 Pages: 237-243
  • Peer Reviewed
• [Journal Article] x線小角散乱でナノ空間を照らし出す (2010)
  • Author(s): 秋山修志
  • Journal Title: 現代化学 468 Pages: 54-58
• [Journal Article] Quality control of protein standards for molecular mass detrminations by small-angle X-ray scattering (2010)
  • Author(s): Shuji Akiyama
  • Journal Title: Journal of Applied Crystallography 43 Pages: 237-243
  • Peer Reviewed
• [Journal Article] X線小角散乱でナノ空間を照らし出す (2010)
  • Author(s): 秋山修志
  • Journal Title: 現代化学 468 Pages: 54-58
• [Journal Article] A Pair of Circularly Permutated PDZ Domains Control RseP, the S2P Family Intramembrane Protease of Escherichia coli (2008)
  • Author(s): Inaba K., Suzuki M., Maegawa K., Akiyama S., Ito K., Akiyama Y.
  • Journal Title: J. Biol. Chem. 283 Pages: 35042-35052
  • Peer Reviewed
• [Journal Article] Assembly and Disassembly Dynamics of the Cyanobacterial Periodosome (2008)
  • Author(s): Akiyama S., Nohara A., Ito K., Maeda Y.
  • Journal Title: Mol. Cell 29 Pages: 703-716
  • Peer Reviewed
• [Journal Article] リアルタイムX線小角散乱で観察した藍藻時計タンパク質の離合集散ダイナミクス (2008)
  • Author(s): 秋山修志
  • Journal Title: 放射光 21 Pages: 305-312
  • NAID: 10024631844
  • Peer Reviewed
• [Journal Article] リアルタイムx線小角散乱でみた藍藻時計タンパク質の離合集散ダイナミクス (2008)
  • Author(s): 秋山修志
  • Journal Title: 生物物理 49 Pages: 135-136
  • NAID: 10025975663
  • Peer Reviewed
• [Journal Article] Real-Time Small-Angle X-Ray Scattering Observation of Assembly and Disassembly Dynamics of Cyanobacterial Periodosome (2008)
  • Author(s): Akiyama S., Nohara A., Ito K., Maeda Y.
  • Journal Title: SPring-8 Research Frontiers Pages: 24-25
  • Peer Reviewed
• [Journal Article] A pair of circularly permutated PDZ domains control RseP, the, S2P family intramembrane protease of E. coli (2008)
  • Author(s): Kenji Inaba
  • Journal Title: Journal of Biological Chemistry 283 Pages: 35042-35052
  • Peer Reviewed
• [Journal Article] Real-time Small-angle X-ray Scattering Observation of Assembly and Disassembly Dynamics of Cyanobacterial Periodosome (2008)
  • Author(s): Shuji Akiyama
  • Journal Title: SPring-8 Kesearch Frontiers 2007 Pages: 24-25
• [Journal Article] Assembly and Disassembly Dynamics of the Cyanobacterial Periodosome
  • Author(s): Shuji Akiyama
  • Journal Title: Molecular Cell
• [Presentation] Reproducible Preparations of Molecular Mass Standards for Small-angle Solution Scattering (2010)
  • Author(s): 秋山修志
  • Organizer: 生物物理学会中部支部講演会
  • Place of Presentation: 岡崎
• [Presentation] Reproducible Preparations of Molecular Mass Standards for Small-angle Solution Scattering (2009)
  • Author(s): Akiyama S.
  • Organizer: XIV International Conference on Small-Angle Scattering
  • Place of Presentation: Oxford, UK
• [Presentation] Real-time Small-Angle X-ray Scattering Study of Assembly and Disassembly Cycles of Cyanobacterial Circadian Clock Proteins (2008)
  • Author(s): Shuji Akiyama
  • Organizer: American Crystallographic Association 2008 Annual Meeting
  • Place of Presentation: Knoxville, TN, USA
  • Year and Date: 2008-06-02