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Development of detection and inducing method for single cell apoptosis and study on blood mineralization with these method

Research Project

Project/Area Number 19770163
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Cell biology
Research InstitutionOsaka University (2009)
The University of Tokyo (2007-2008)

Principal Investigator

KIHARA Takanori  The University of Tokyo, 大学院・基礎工学研究科, 助教 (90436535)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,740,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥540,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsナノプローブ / 原子間力顕微鏡 / アポトーシス / 一細胞 / TRAIL / ナノ針 / 石灰化 / 単一生細胞内mRNA検出 / モレキュラービーコン / 単一生細胞内酵素活性 / MPCポリマー / FRET型センサー分子 / 血管石灰化
Research Abstract

I have been developed the single cell apoptosis detection method using nanoprobe. The nanoprobe for enzyme or mRNA was newly constructed and I have succeeded in detection of apoptosis in a single cell by insertion the nanoprobe to the living cell. Moreover, I tried to develop the apoptosis inducing method in a single cell. As constructing the apoptosis ligand modified probe, I succeeded in detection of specific interaction between the apoptosis ligand and receptor on a single cell surface.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (46 results)

All 2009 2008 2007 Other

All Journal Article (12 results) (of which Peer Reviewed: 10 results) Presentation (34 results)

  • [Journal Article] Development of a method to evaluate caspase-3 activity in a single cell using a nanoneedle and a fluorescent probe2009

    • Author(s)
      Kihara, T., Nakamura, C., Suzuki, M., Han, S.W., Fukazawa, K., Ishihara, K., Miyake, J.
    • Journal Title

      Biosensors and Bioelectronics 25

      Pages: 22-27

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanical role of the nucleus in a cell in terms of elastic modulus2009

    • Author(s)
      Sugitate, T., Kihara, T., Liu, X.Y., Miyake, J.
    • Journal Title

      Current Applied Physics 9

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a method to evaluate caspase-3 activity in a single cell using a nanoneedle and a fluorescent probe2009

    • Author(s)
      KIHARA, Takanori
    • Journal Title

      Biosensors and Bioelectronics 25

      Pages: 22-27

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanical role of the nucleus in a cell in terms of elastic modulus2009

    • Author(s)
      SUGITATE, Toshihiro
    • Journal Title

      Current Applied Physics 9

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞操作技術 遺伝子導入技術2009

    • Author(s)
      木原隆典
    • Journal Title

      Medical Bio (September)

      Pages: 46-49

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Modulation of SRF-dependent gene expression by association of SPT16 with MKL12008

    • Author(s)
      Kihara, T., Kano, F., Murata, M.
    • Journal Title

      Experimental Cell Research 314

      Pages: 629-637

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intercellular accumulation of type V collagen fibrils in accordance with cell aggregation2008

    • Author(s)
      KIHARA, Takanori
    • Journal Title

      Journal of Biochemistry 144

      Pages: 625-633

    • NAID

      10024913455

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of SRF-dependent gene expression by association of SPT16 with MKL12008

    • Author(s)
      KIHARA, Takanori
    • Journal Title

      Experimental Cell Research 314

      Pages: 629-637

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ナノテクノロジーを用いた細胞操作2008

    • Author(s)
      三宅 淳
    • Journal Title

      分死細胞治療 7

      Pages: 18-25

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Nanoneedle surface modification with 2-methacryloyloxyethyl phosphorylcholine polymer to reduce nonspecific protein adsorption in a living cell2007

    • Author(s)
      Kihara, T., Yoshida, N., Mieda, S., Fukuzawa, K., Nakamura, C., Ishihara, K., Miyake, J.
    • Journal Title

      NanoBiotechnology 3

      Pages: 127-134

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nanoneedle surface modification with 2-methacryloyloxyethyl phosphorylcholine polymer to reduce nonspecific protein adsorptio2007

    • Author(s)
      KIHARA, Takanori
    • Journal Title

      NanoBiotechnology 3

      Pages: 127-134

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Successive detection of insulin-like growth factor-II bound to receptors on a living cell surface using an AFM

    • Author(s)
      HAN, Sung-Woong
    • Journal Title

      Journal of Molecular Recognition (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Development of a detection method for intracellular mRNA using in situnanoprobe2009

    • Author(s)
      KIHARA, Takanori
    • Organizer
      APBioChEC'09
    • Place of Presentation
      Kobe, Hyogo
    • Year and Date
      2009-11-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] AFMを用いたバイオ分子・細胞の力学的計測2009

    • Author(s)
      三宅淳
    • Organizer
      日本機械学会第20回バイオフロンティア講演会
    • Place of Presentation
      和歌山県民文化会館, 和歌山
    • Year and Date
      2009-11-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] 先鋭化AFM探針の貫通現象と細胞表層構造との関係について2009

    • Author(s)
      木原隆典
    • Organizer
      日本機械学会 第20回バイオフロンティア講演会
    • Place of Presentation
      和歌山県民文化会館, 和歌山
    • Year and Date
      2009-11-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] A novel method of cell discrimination by mechanical detection between cytoskeletal marker protein and antibody immobilized on nanoneedle2009

    • Author(s)
      MIEDA, Shingo
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      徳島文理大学, 徳島
    • Year and Date
      2009-11-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] Molecuiar Beacon修飾ナノ針を用いた生細胞のmRNA解析2009

    • Author(s)
      中村史
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋大学, 愛知
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 異なる先端サイズのAFMプローブを用いた細胞表層構造の評価2009

    • Author(s)
      杉立年弘
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋大学, 愛知
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 抗体修飾ナノ針を用いた生細胞のマーカータンパク質解析2009

    • Author(s)
      三枝真吾
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋大学, 愛知
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] カーボンナノチューブフォレストを電子供与媒体としたヒドロゲナーゼからの電気化学的水素発生デバイスの作成2009

    • Author(s)
      木原隆典
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋大学, 愛知
    • Year and Date
      2009-09-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] モレキュラービーコン修飾ナノ針によるOct4 mRNAの検出2009

    • Author(s)
      金百合恵
    • Organizer
      日本化学会 第24回生体機能関連化学シンポジウム,第12回バイオテクノロジー部会シンポジウム
    • Place of Presentation
      九州大学, 福岡
    • Year and Date
      2009-09-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] モレキュラービーコン修飾ナノ針を用いた細胞内mRNAの解析2009

    • Author(s)
      中村史
    • Organizer
      日本化学会 第24回生体機能関連化学シンポジウム,第12回バイオテクノロジー部会シンポジウム
    • Place of Presentation
      九州大学, 福岡
    • Year and Date
      2009-09-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] ナノ針の細胞挿入効率に対する積層ナノ薄膜の効果2009

    • Author(s)
      河野景子
    • Organizer
      日本化学会 第24回生体機能関連化学シンポジウム,第12回バイオテクノロジー部会シンポジウム
    • Place of Presentation
      九州大学, 福岡
    • Year and Date
      2009-09-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] Molecular Beacon修飾ナノ針を用いた細胞内mRNAの検出2009

    • Author(s)
      中村史
    • Organizer
      2009年電気化学秋季大会
    • Place of Presentation
      東京農工大学, 東京
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細胞内マーカータンパク質の免疫力学測定による細胞選別法の開発2009

    • Author(s)
      三枝真吾
    • Organizer
      2009年電気化学秋季大会
    • Place of Presentation
      東京農工大学, 東京
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] A method of mechanical force detection of insulin-like growth factor-II bound to receptors on a living cell surface using an AFM2009

    • Author(s)
      NAKAMURA, Chikashi
    • Organizer
      ENGINEERING CELL BIOLOGY III
    • Place of Presentation
      Santa Cruz, California, USA
    • Year and Date
      2009-08-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] AFMを用いた細胞内分子検出技術の開発2009

    • Author(s)
      木原隆典, 他
    • Organizer
      第2回ナノバイオ若手ネットワーキングシンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2009-06-12
    • Related Report
      2009 Final Research Report
  • [Presentation] AFMを用いた細胞内分子検出技術の開発2009

    • Author(s)
      木原隆典
    • Organizer
      第2回ナノバイオ若手ネットワーキングシンポジウム
    • Place of Presentation
      関西セミナーハウス, 京都
    • Year and Date
      2009-06-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] Culture conditions affecting cellular clump formation accompanying intercellular accumulation of type V collagen fibrils2009

    • Author(s)
      UCHIDA, Kenji
    • Organizer
      8th Pan-Pacific Connective Tissue Societies Symposium
    • Place of Presentation
      Yokosuka, Kanagawa
    • Year and Date
      2009-06-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] Intercellular accumulation of type V collagen fibrils in accordance with cell aggregation2009

    • Author(s)
      KIHARA, Takanori
    • Organizer
      8th Pan-Pacific Connective Tissue Societies Symposium
    • Place of Presentation
      Yokosuka, Kanagawa
    • Year and Date
      2009-06-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] Development of in situ molecular sensor by using nanoneedle technology2008

    • Author(s)
      Kihara, T., et al.
    • Organizer
      NanoBio Seoul 2008
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2008-10-31
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Development of in situ FRET sensor for cell apoptosis by using nanoneedle technology2008

    • Author(s)
      Kihara, T., et al.
    • Organizer
      Biosensors 2008
    • Place of Presentation
      Shanghai, China
    • Year and Date
      2008-05-14
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] キメラ型FRETセンサー蛋白質の構築とカスパーゼ応答の評価2008

    • Author(s)
      木原 隆典
    • Organizer
      日本化学会第88春季年会
    • Place of Presentation
      立教大学,東京
    • Year and Date
      2008-03-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] In situナノプローブを用いたRNA解析2008

    • Author(s)
      木原 隆典
    • Organizer
      日本化学会第88春季年会
    • Place of Presentation
      立教大学,東京
    • Year and Date
      2008-03-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] Development of FRET based in situ sensor for caspase-3 by using nanoneedle technology2008

    • Author(s)
      KIHARA, Takanori
    • Organizer
      2nd AFM BioMed Conference
    • Place of Presentation
      Monterey, LA, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Development of FRET based in situ sensor for caspase-3 by using nanoneedle technology2008

    • Author(s)
      KIHARA, Takanori
    • Organizer
      細胞情報国際シンポジウム
    • Place of Presentation
      Kobe, Hyogo
    • Related Report
      2008 Annual Research Report
  • [Presentation] Development of a caspase-3 probe functioned on the surface of nanoneedle to evaluate the enzyme activities in single living cell2008

    • Author(s)
      木原隆典
    • Organizer
      第46回生物物理学会年会
    • Place of Presentation
      博多, 福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] 単一生細胞内での酵素活性定量のための固相型プローブの開発2008

    • Author(s)
      木原隆典
    • Organizer
      第60回日本生物工学会大会
    • Place of Presentation
      仙台, 宮城
    • Related Report
      2008 Annual Research Report
  • [Presentation] Cell Surgeryを利用した1細胞内分子計測技術MOMENT法の開発2008

    • Author(s)
      木原隆典
    • Organizer
      分子情報生命科学スクーリング
    • Place of Presentation
      和光, 埼玉
    • Related Report
      2008 Annual Research Report
  • [Presentation] AFMを用いた細胞操作技術(セルサージェリー)2008

    • Author(s)
      木原隆典
    • Organizer
      ナノバイオ若手NWシンポジウム
    • Place of Presentation
      三島, 静岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] In situナノプローブを用いたRNA解析2008

    • Author(s)
      木原隆典
    • Organizer
      第60回日本生物工学会大会
    • Place of Presentation
      仙台, 宮城
    • Related Report
      2008 Annual Research Report
  • [Presentation] Surface modification of nanoneedle with 2-methacryloyloxyethyl phosphorylchorine (MPC) polymers for improving the biocompatibility with cell interior2007

    • Author(s)
      HIHARA, Takanori
    • Organizer
      第45回生物物理学会年会
    • Place of Presentation
      パシフィコ横浜,神奈川
    • Year and Date
      2007-12-21
    • Related Report
      2007 Annual Research Report
  • [Presentation] Development of nanoprobe for inner cell function2007

    • Author(s)
      Kihara, T., et al.
    • Organizer
      CNSI-CNBI Symposium on NanoBiotechnology
    • Place of Presentation
      LA, USA
    • Year and Date
      2007-11-01
    • Related Report
      2009 Final Research Report
  • [Presentation] Cell Surgery2007

    • Author(s)
      KIHARA, Takanori
    • Organizer
      Swiss-Japan Symposium on Nanobio 2007
    • Place of Presentation
      The University of Tokyo, Tokyo
    • Year and Date
      2007-10-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] MPC(2-methacryloyloxyethyl phosphorylchorine)ポリマーを用いたナノ針表面修飾効果の検討2007

    • Author(s)
      木原 隆典
    • Organizer
      第59回日本生物工学会大会
    • Place of Presentation
      広島大学,広島
    • Year and Date
      2007-09-25
    • Related Report
      2007 Annual Research Report
  • [Presentation] Development of nanoprobe for inner cell function2007

    • Author(s)
      KIHARA, Takanori
    • Organizer
      CNSI-CNBI Symposium on NanoBiotechnology
    • Place of Presentation
      UCLA, LA, USA
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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