Development of detection and inducing method for single cell apoptosis and study on blood mineralization with these method
| Project/Area Number |
19770163
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Osaka University (2009)
The University of Tokyo (2007-2008) |
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Principal Investigator |
KIHARA Takanori The University of Tokyo, 大学院・基礎工学研究科, 助教 (90436535)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,740,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥540,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | ナノプローブ / 原子間力顕微鏡 / アポトーシス / 一細胞 / TRAIL / ナノ針 / 石灰化 / 単一生細胞内mRNA検出 / モレキュラービーコン / 単一生細胞内酵素活性 / MPCポリマー / FRET型センサー分子 / 血管石灰化 |
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| Research Abstract |
I have been developed the single cell apoptosis detection method using nanoprobe. The nanoprobe for enzyme or mRNA was newly constructed and I have succeeded in detection of apoptosis in a single cell by insertion the nanoprobe to the living cell. Moreover, I tried to develop the apoptosis inducing method in a single cell. As constructing the apoptosis ligand modified probe, I succeeded in detection of specific interaction between the apoptosis ligand and receptor on a single cell surface.
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Report
(4 results)
Research Products
(46 results)
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[Journal Article] Development of a method to evaluate caspase-3 activity in a single cell using a nanoneedle and a fluorescent probe2009
Author(s)
Kihara, T., Nakamura, C., Suzuki, M., Han, S.W., Fukazawa, K., Ishihara, K., Miyake, J.
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Journal Title
Biosensors and Bioelectronics 25
Pages: 22-27
Related Report
Peer Reviewed
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[Journal Article] Nanoneedle surface modification with 2-methacryloyloxyethyl phosphorylcholine polymer to reduce nonspecific protein adsorption in a living cell2007
Author(s)
Kihara, T., Yoshida, N., Mieda, S., Fukuzawa, K., Nakamura, C., Ishihara, K., Miyake, J.
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Journal Title
NanoBiotechnology 3
Pages: 127-134
Related Report
Peer Reviewed
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