A study of gene targeting method in amphibians

Research Project

Project/Area Number	19770191
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Developmental biology
Research Institution	The University of Tokyo
Principal Investigator	ONUMA Yasuko The University of Tokyo, 幹細胞工学研究センター, 研究員 (90431824)
Project Period (FY)	2007 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,120,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥720,000) Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywords	発生・分化 / 動物 / 遺伝学 / 細胞・組織 / 両生類
Research Abstract	The main aim is establishment of a pluripotent stem cell line as a basic technology for gene targeting in amphibians. For establishment of embryonic germ (EG) cell line, the tissue piece including fluorescent-marked primordial germ cells (PGC) was isolated by manipulation from embryos and was preferred to examine culture condition. Using early embryos and primary cell culture, gene-transfer and culture methods were investigated to produce induced pluripotent stem cell (iPS cell).