Functional analysis of rice flowering genes on the vegetative growth of rice.

• Project/Area Number: 19780003
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: TSUJI Hiroyuki Nara Institute of Science and Technology, バイオサイエンス研究科, 助教 (40437512)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,880,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: イネ / フロリゲン

Research Abstract

フロリゲンは植物の開花を調節する基本分子として約70年前に提唱された。2007年にその実体がイネではHd3aタンパク質であることが明らかとなった。Hd3aタンパク質はイネの出穂促進条件である短日条件下で葉身の維管束において合成され、維管束を経由して茎頂まで移動し、茎頂分裂組織の生長相転換を促す。本研究では、Hd3a-GFP融合遺伝子を維管束で発現させたイネにおいて、フロリゲンの増加による極早生の表現型に加えて分げつの増加、半矮性、穂の分枝の減少など様々な形態異常を示すことを見いだした。

Research Products

• [Journal Article] 花成ホルモン, フロリゲン-その構造解析から分子機構の理解へ (2009)
  • Author(s): 田岡健一郎、辻寛之、島本功
  • Journal Title: 蛋白質核酸酵素 54 Pages: 1702-1707
• [Journal Article] The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a. (2009)
  • Author(s): Purwestri, Y.A., Ogaki, Y., Tamaki, S., Tsuji, H., Shimamoto, K.
  • Journal Title: Plant Cell Physiol 50 Pages: 429-438
  • NAID: 10027338030
  • Peer Reviewed
• [Journal Article] The 14-3-3 protein GF14c acts as a negative rugulator of flowering in rice by interacting with the florigen Hd3a. (2009)
  • Author(s): Purwestri, Y.A.
  • Journal Title: Plant Cell Physiol. 50 Pages: 429-438
  • Peer Reviewed
• [Journal Article] Florigen and photoperiodic control of flowering in rice. (2008)
  • Author(s): Tsuji, H., Tamaki, S., Komiya, R., Shimamoto, K.
  • Journal Title: RICE 1 Pages: 25-35
  • Peer Reviewed
• [Journal Article] Florigen and photoperiodic control of flowering in rice. (2008)
  • Author(s): Tsuji, H.
  • Journal Title: RICE 1 Pages: 25-35
  • Peer Reviewed
• [Presentation] Hd3a florigen distribution in the shoot apical meristem during flower development in rice. (2009)
  • Author(s): 玉置祥二郎, 辻寛之, 島本功
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜横浜市
  • Year and Date: 2009-12-09
• [Presentation] イネのフロリゲンHd3aによる分枝の促進 (2009)
  • Author(s): 辻寛之、橘知夏、玉置祥二郎、島本功
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-23
• [Presentation] 島本功イネのフロリゲンHd3aと相互作用する14-3-3タンパク質の解析 (2008)
  • Author(s): 大垣友香, 柳瀬朋子, Yekti Asih Purwestri, 田岡健一郎, Hann Ling Wong, 辻寛之
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド兵庫県
  • Year and Date: 2008-12-09
• [Presentation] The role of Hd3a, a mobile flowering signal, in the architecture of rice (2008)
  • Author(s): Tsuji, H., Tachibana, C., Tamaki, S., Shimamoto, K.
  • Organizer: The 6th International symposium on ricefunctional genomics
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2008-11-09
• [Presentation] The role of Hd3a, a mobile flowering signal, in the architecture of rice (2008)
  • Author(s): Tsuji H.
  • Organizer: The 6th International symposium on ricefunctional genomics
  • Place of Presentation: 韓国
  • Year and Date: 2008-11-09
• [Presentation] Hiroyuki Tsuji and Ko Shimamoto Identification of 14-3-3 protein as an Hd3a interactor in rice Keystone Symposia (2008)
  • Author(s): Yekti Asih Purwestri, Shojiro Tamaki, Yuka Ogaki
  • Place of Presentation: Colorado, USA
  • Year and Date: 2008-09-23
• [Presentation] 島本功 イネの移動性花成シグナルHd3aは花以外の形態形成にも関与する (2007)
  • Author(s): 橘知夏、辻寛之、玉置祥二郎
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: パシフィコ横浜 横浜市
  • Year and Date: 2007-12-13
• [Presentation] Shuji Yokoi and Ko Shimamoto The role of Hd3 (2007)
  • Author(s): Hiroyuki Tsuji, Shojiro Tamaki, Shoichi Matsuo, Chika Tachibana
  • Organizer: a mobile flowering signal, in the architecture of rice The fifth international symposium on rice functional genomics
  • Place of Presentation: エポカルつくば茨城県
  • Year and Date: 2007-10-15