Novel method for the embryo sexing using live-cell imaging

• Project/Area Number: 19780248
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: YAMAGATA Kazuo The Institute of Physical and Chemical Research, ゲノムリプログラミング・研究チーム, 研究員 (10361312)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 雌雄産み分け / ライブセルイメージング / X染色体不活性化 / 着床前初期胚 / 雌雄胚選別 / 初期胚

Research Abstract

雌雄産み分けは、家畜動物の効率的生産という意味で非常に重要な技術であり、特に胚移植前の段階で雌雄胚選別することが望まれる。本研究では細胞にダメージを与えないライブセルイメージング技術を応用し、性染色体を認識する蛍光プローブを開発することで新規雌雄初期胚選別技術の開発を目指した。期間中、雌胚特異的に見られるX染色体不活性化現象をリポートする蛍光プローブを開発し、実際に雌雄胚選別を試みたところ、マウス胚において雌雄の100%の産み分けに成功した。なお、本技術は産仔に対して無害であり、かつ遺伝子組み換え動物にはならないことから、現場への応用が期待される。

Research Products

• [Journal Article] Long-term, Six-dimensional Live-cell Imaging for the Mouse Preimplantation Embryo That Does Not Affect Full-term Development. (2009)
  • Author(s): Yamagata K, Suetsugu R, Wakayama T.
  • Journal Title: Journal of Reproduction and Development 55 Pages: 328-331
  • NAID: 10024942729
  • Peer Reviewed
• [Journal Article] Long-term, Six-dimensional Live-cell Imaging for the Mouse Preimplantation Embryo That Does Not Affect Full-term Development (2009)
  • Author(s): Yamagata K, Suetsugu R, Wakayama T.
  • Journal Title: Journal of Reproduction and Development 55(In press)
  • NAID: 10024942729
  • Peer Reviewed
• [Journal Article] Captuing Epigenetic Dynamics during Preimplantation Development Using Live Cell Imaging. (2008)
  • Author(s): Yamagata K
  • Journal Title: Journal of Biochemistry 143 Pages: 279-86
• [Journal Article] Nuclear localization of profilin III-ArpM1 complex in mouse spermiogenesis. (2008)
  • Author(s): Hara Y, Yamagata K, Oguchi K Baba T.
  • Journal Title: FEBS letters 582 Pages: 2998-3004
  • Peer Reviewed
• [Journal Article] Cd52, known as a major maturation-associated sperm membrane antigensecrted from the epididymis, is not required for fertilization in the mouse. (2008)
  • Author(s): Yamaguchi R, Yamagata K, Hasuwa H, Inano E, Ikawa M, Okabe M.
  • Journal Title: Genes to Cells 13 Pages: 851-61
  • Peer Reviewed
• [Journal Article] The fusing ability of sperm is bestowed by CD9-containing vesicles released from eggs in mice. (2008)
  • Author(s): Miyado K, Yoshida K, Yamagata K, et al.
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 12921-6
  • Peer Reviewed
• [Journal Article] Increasing the Cell Number of Host Tetraploid Embros Ca Improve the Production of Mice Derived from Embryonic Stem Cells. (2008)
  • Author(s): Ohta H, Sakaide Y, Yamagata K, Wakayama T.
  • Journal Title: Biology of Reproduction 79 Pages: 486-92
  • Peer Reviewed
• [Journal Article] Nuclear localization of profilin III-ArpMl complex in mouse spermiogenesis (2008)
  • Author(s): Hara Y, Yamagata K, Oguchi K, Baba T.
  • Journal Title: FEBS letters 582 Pages: 2998-3004
  • Peer Reviewed
• [Journal Article] Cd52, known as a major maturation-associated sperm membrane antigen secreted from the epididymis, is not required for fertilization in the mouse (2008)
  • Author(s): Yamaguchi R, Yamagata K, Hasuwa H, Inano E, Ikawa M, Okabe M.
  • Journal Title: Genes to Cells 13 Pages: 851-61
  • Peer Reviewed
• [Journal Article] The fusing ability of sperm is bestowed by CD9-contairiing vesicles released from eggs in mice (2008)
  • Author(s): Miyado K, Yoshida K, Yamagata K, et al.
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 12921-6
  • Peer Reviewed
• [Journal Article] In creasing the Cell Number of Host Tetraploid Embryos Can Improve the Production of Mice Derived from Embryonic Stem Cells (2008)
  • Author(s): Ohta H, Sakaide Y, Yamagata K, Wakayama T.
  • Journal Title: Biology of Reproduction 79 Pages: 486-92
  • Peer Reviewed
• [Journal Article] Capturing Epigenetic Dynamics during Preimplantation Development Using Live Cell Imaging. (2008)
  • Author(s): Kazuo Yamagata
  • Journal Title: Journal of Biochemistry (In press)
  • NAID: 10024908457
• [Journal Article] Assessment of chromosome itegrity using a novel live-cell imaging technique in mouse embryosproduced by intracytoplasmic sperm injection.
  • Author(s): Yamagata K, Suetsugu R, Wakayama T.
  • Journal Title: Human Reproduction (In press)
  • Peer Reviewed
• [Presentation] 着床前初期胚ライブセルイメージング技術の開発と胚診断法への応用 (2008)
  • Author(s): 山縣一夫
  • Organizer: 第53回日本生殖医学会総会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-10-24
• [Presentation] ライブセルイメージングを用いたマウス着床前初期胚の運命予測 (2008)
  • Author(s): 山縣一夫
  • Organizer: 第101回繁殖生物学会大会
  • Place of Presentation: 九州大学医学部百年講堂
  • Year and Date: 2008-09-20
• [Presentation] ライブセルイメージングを用いた々ウス着床前初期胚の運命予測 (2008)
  • Author(s): 山縣一夫
  • Organizer: 第101回繁殖生物学会大会
  • Place of Presentation: 九州大学医学部百年講堂
  • Year and Date: 2008-09-20
• [Presentation] ライブセルイメージングによる受精・初期胚発生時のエピジェネティクス変化の解析 (2007)
  • Author(s): 山縣一夫
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] Live-cell imaging technique for the preimplantation embryos and its application. (2007)
  • Author(s): Kazuo Yamagata
  • Organizer: Asian Reproductive Biotechnology Society
  • Place of Presentation: Singapore, National University of Singapore
  • Year and Date: 2007-11-26
• [Presentation] Live-cell imaging technique for the preimplantation embryos and its application (2007)
  • Author(s): Kazuo Yamagata
  • Organizer: Asian Reproductive Biotechnology Society
  • Place of Presentation: Singapore, National University of Singapore
  • Year and Date: 2007-11-26
• [Presentation] 哺乳動物初期胚ライブセルイメージングシステムの開発と応用 (2007)
  • Author(s): 山縣一夫
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 東京大学農学部
  • Year and Date: 2007-10-21
• [Presentation] マウス初期胚のライブセルイメージングにより見えてきたもの (2007)
  • Author(s): 山縣一夫
  • Organizer: 日本受精着床学会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2007-08-30
• [Book] 顕微鏡なるほどQ&A、6章 観察(応用編)に関するQ&A (2008)
  • Author(s): 山縣一夫
  • Total Pages: 24
  • Publisher: 羊土社
• [Book] 顕微鏡なるほどQ&A (2008)
  • Author(s): 山縣一夫
  • Total Pages: 24
  • Publisher: 羊土社
• [Book] 蛋白質核酸酵素増刊号「生殖細胞の発生・エピジェネティクスと再プログラム化 (2007)
  • Author(s): 山縣一夫, ら
  • Total Pages: 7
  • Publisher: 共立出版
• [Book] 蛋白質核酸酵素増刊号「生殖細胞の発生・エピジェネティクスと再プログラム化」 (2007)
  • Author(s): 山縣一夫, ら
  • Total Pages: 7
  • Publisher: 共立出版
• [Patent(Industrial Property Rights)] 哺乳動物胚のインビトロ蛍光観察方法及び着床不全又は流産のリスクの低い哺乳動物胚の選択方法 (2008)
  • Inventor(s): 山縣一夫、若山照彦
  • Industrial Property Rights Holder: 理化学研究所
  • Industrial Property Number: 2008-213296
  • Filing Date: 2008-08-21
• [Patent(Industrial Property Rights)] 哺乳動物胚のインビトロ蛍光観察方法及び着床不全又は流産のリスクの低い哺乳動物胚の選択方法 (2008)
  • Inventor(s): 山縣一夫, 若山照彦
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Industrial Property Number: 2008-213296
  • Filing Date: 2008-08-21