Myosin 1E regulates the cell morphology

• Project/Area Number: 19790061
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Nagasaki University
• Principal Investigator: TANIMURA Susumu Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (90343342)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 細胞生物学 / ミオシン / シグナル伝達 / 細胞形態 / 細胞運動 / 細胞内輸送

Research Abstract

ミオシン1EはTail Homology(TH)2領域を介してホスファチジルイノシトール(4,5)二リン酸[PI(4,5)P2]、あるいはPI(3,4,5)P3と結合し、モータードメインを介してアクチンフィラメント上を移動することで、細胞運動先端部位における細胞形態を調節することを見出した。また、ミオシン1EのTH2領域に結合する新規タンパク質としてSH3P2を見出し、それがミオシン1Eの機能調節因子である可能性を提示した。

Research Products

• [Journal Article] Blockade of constitutively activated ERK signaling enhances cytotoxicity of microtubule-destabilizing agents in tumorcells (2009)
  • Author(s): Tanimura S., Uchiyama A., Watanabe K., Yasunaga M., Inada Y., Kawabata T., Iwashita K., Noda S., Ozaki K., Kohno M.
  • Journal Title: Biochem. Biophys. Res. Commun. 378 Pages: 650-655
  • Peer Reviewed
• [Journal Article] GEF-H1 mediates tumor necrosis factor-aindunced Rho activation and myosin phosphorylation : Role in the regulation of tubular paracellular permeability (2009)
  • Author(s): Kakiashvili E., Speight P., Waheed F., Seyh R., Lodyga M., Tanimura S., Kohno M., Rotstein OD., Kapus A., Szaszi K.
  • Journal Title: J. Biol. Chem. (in press)
  • Peer Reviewed
• [Journal Article] ERK regulates renal cell proliferation and renal cyst expression in inv mutant mice (2009)
  • Author(s): Okumura Y., Sugiyama N., Tanimura S., Nishida M., Hamaoka K., Kohno M., Yokoyama T.
  • Journal Title: Acta Histochem. Cytochem. (in press)
  • Peer Reviewed
• [Journal Article] Blockade of constitutively activated ERK signaling enhances cytotoxity of microtubuledestabilizing agents in tumor cells (2009)
  • Author(s): Tanimura, S.
  • Journal Title: Biochem. Biophys. Res. Commun. 378 Pages: 650-655
  • Peer Reviewed
• [Journal Article] GEE-HI mediates tumor necrosis factoraindunced Rho activation and myosin phosphorylation : Role in the regulation of tubular paracellular permeability (2009)
  • Author(s): Kakiashvili, E.
  • Journal Title: J. Biol. Chem. (in press)(掲載確定)
  • Peer Reviewed
• [Journal Article] ERK regulates renal cell proliferation and renal cyst expression in invmutant mice (2009)
  • Author(s): Okumura, Y.
  • Journal Title: Acta Histochem. Cytochem. (in press)(掲載確定)
  • Peer Reviewed
• [Journal Article] ERK1/2 phosphorylates GEF-H1 to enhance its guanine nucleotide exchange activity toward RhoA (2008)
  • Author(s): Fujishiro SH., Tanimura S., Mure S., Kashimoto Y., Watanabe K., Kohno M.
  • Journal Title: Biochem. Biophys. Res. Commun. 368 Pages: 162-167
  • Peer Reviewed
• [Journal Article] Histone deacetylase inhibitors enhance the chemosensitivity of tumor cells with crossresistance to a wide range of DNA-damaging drugs. (2008)
  • Author(s): Ozaki K., Kishikawa F., Tanaka M., Sakamoto T., Tanimura S., Kohno M.
  • Journal Title: Cancer Sci. 99 Pages: 376-384
  • NAID: 10024002722
  • Peer Reviewed
• [Journal Article] ERK1/2 phosphorylate GEF-H1 to enhance its guanine nucleotide exchange activity toward RhoA. (2008)
  • Author(s): Fujishiro, SH.
  • Journal Title: Biochem. Biophys. Res. Commun. 368 Pages: 162-167
  • Peer Reviewed
• [Journal Article] Histone deacetylase inhibitors enhance the chemosensitivity of tumor cells with cross-resistance to a wide range of DNA-damaging drugs. (2008)
  • Author(s): Ozaki, K.
  • Journal Title: Cancer Sci. 99 Pages: 376-384
  • Peer Reviewed
• [Journal Article] Anticancer drugs up-regulate HspBP1 and thereby antagonize the prosurvival function of Hsp70 in tumor cells (2007)
  • Author(s): Tanimura S., Hirano A., Hashizume J., Yasunaga M., Kawabata T., Ozaki K., Kohno M.
  • Journal Title: J. Biol. Chem. 282 Pages: 35430-35439
  • Peer Reviewed
• [Journal Article] Anticancer drugs up-regulate HspBP1 and thereby antagonize the prosurvival function of Hsp70 in tumor cells. (2007)
  • Author(s): Tanimura, S.
  • Journal Title: J. Biol. Chem. 282 Pages: 35430-35439
  • Peer Reviewed
• [Presentation] SH3P2はMMP-9、OPNの発現抑制を介して浸潤能を負に制御する (2009)
  • Author(s): 橋詰淳哉
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-26
• [Presentation] Myo1Eはホスホイノシタイドとの結合を介して細胞形態を制御する (2008)
  • Author(s): 瀬井香奈子
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] SH3ドメインを含む新規タンパク質(p26)による細胞運動制御の分子機構 (2008)
  • Author(s): 黒崎由希子
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Myo1Eはホスホイノシタイドとの結合を介して細胞形態を制御する (2008)
  • Author(s): 瀬井香奈子
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-12-11
• [Presentation] SH3ドメインを含む新規タンパク質(p26)による細胞運動制御の分子機構 (2008)
  • Author(s): 黒崎由希子
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-12-11
• [Presentation] A novel SH3-containing 30 kDa protein suppresses the invasiveness of tumor cells by inhibiting the secretion of MMP-9 (2008)
  • Author(s): Tanimura S.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] A novel SH3containing 30 1cDa protein suppresses the invasiveness of tumor cells by inhibiting the secretion of MMP-9 (2008)
  • Author(s): Tanimura, S.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Myosin 1Eは細胞形態の制御に関与する分子である (2007)
  • Author(s): 渡司雅弘
  • Organizer: 日本薬学会九州支部大会
  • Place of Presentation: 福岡
  • Year and Date: 2007-12-08
• [Presentation] Myosin1Eは細胞形態の制御に関与する分子である (2007)
  • Author(s): 渡司 雅弘
  • Organizer: 日本薬学会九州支部大会
  • Place of Presentation: 福岡
  • Year and Date: 2007-12-08