Combined exercise and antioxidanttherapy for amyotrophic lateral sclerosis model mice.

• Project/Area Number: 19790603
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurology
• Research Institution: Gifu University
• Principal Investigator: HAYASHI Yuichi Gifu University, 大学院・医学系研究科, 助教 (00392366)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,680,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: ALS / 遺伝子治療 / 抗酸化物質 / メタロチオネイン / 運動療法 / 抗酸化作用 / 逆行輸送 / 筋萎縮性側索硬化症 / リハビリテーション / モデル動物 / メタロチオネイン-I / メタロチオネイン-III / アデノウイルス

Research Abstract

筋萎縮性側索硬化症モデルマウスに対するmetallothinein(MT)組み換えアデノウイルス遺伝子治療は極めて有効で、生存期間延長効果がみられた。
また運動療法に関しては、脊髄前角の運動ニューロンにおいて、MT-I, II,IIIの誘導を認めた。このことは、運動療法の有用性の科学的なメカニズムの一旦として、MTの関与を示唆するものであった。

Research Products

• [Journal Article] Serial monitoring of basal metabolic rate for therapeutic evaluation in an Isaacs' syndrome patient with chronic fluctuating symptoms. (2010)
  • Author(s): Hayashi Y, et al.
  • Journal Title: Internal Med 49 Pages: 475-477
  • NAID: 130000251699
  • Peer Reviewed
• [Journal Article] Markedly ring-enhanced optic nerves due to metastasis of signet-ring cell gastric carcinoma. (2010)
  • Author(s): Hayashi Y, et al.
  • Journal Title: Internal Med 49 Pages: 517-517
  • NAID: 130000251711
  • Peer Reviewed
• [Journal Article] High prevalence of autoantibodies against phosphglycerate mutase 1 in patients with autoimmune central nervous system diseases. (2010)
  • Author(s): Kimura A, Sakurai T, Koumura A, Yamada M, Hayashi Y, et al.
  • Journal Title: J Neuroimmunol 219 Pages: 105-108
  • Peer Reviewed
• [Journal Article] Antibodies in patients with neuropsychiatric systemic lupus erythematosus. (2010)
  • Author(s): Kimura A, Kanoh Y, Sakurai T, Koumura A, Yamada M, Hayashi Y, et al.
  • Journal Title: Neurology Pages: 1327-1329
  • Peer Reviewed
• [Journal Article] Motor-dominant chronic inflammatory demyelinating polyneuropathy. (2010)
  • Author(s): Kimura A, Sakurai T, Koumura A, Yamada M, Hayashi Y, et al.
  • Journal Title: J Neurol 257 Pages: 612-619
  • Peer Reviewed
• [Journal Article] Serial monitoring of basal metabolic rate for therapeutic evaluation in an Isaacs' syndrome patient with chronic fluctuating symptoms. (2010)
  • Author(s): Hayashi Y, Kimura A, Watanabe N, Yamada M, Sakurai T, Tanaka Y, Hozumi I, Inuzuka T.
  • Journal Title: Internal Med 49 Pages: 475-477
  • NAID: 130000251699
  • Peer Reviewed
• [Journal Article] Markedly ring-enhanced optic nerves due to metastasis of signet-ring cell gastric carcinoma. (2010)
  • Author(s): Hayashi Y, Kato T, Tanaka Y, Yamada M, Koumura A, Kimura A, Hozumi I, Inuzuka T.
  • Journal Title: Internal Med 49 Pages: 517-517
  • NAID: 130000251711
  • Peer Reviewed
• [Journal Article] High prevalence of autoantibodies against phosphglycerate mutase 1 inpatients with autoimmune central nervous system diseases. (2010)
  • Author(s): Kimura A, Sakurai T, Koumura A, Yamada M, Hayashi Y, Tanaka Y, Hozumi I, Tanaka R, Takemura M, Seishima M, Inuzuka T.
  • Journal Title: J Neuroimmunol 219 Pages: 105-108
  • Peer Reviewed
• [Journal Article] Exercise induces metallothioneins in mouse spinal cord. (2009)
  • Author(s): Hashimoto K, Hayashi Y, et al.
  • Journal Title: Neuroscience 163 Pages: 244-51
  • Peer Reviewed
• [Journal Article] DNA microarray analysis of transcriptional responses of mouse spinal cords to physical exercise. (2009)
  • Author(s): Hashimoto K, Honda A, Hayashi Y, et al.
  • Journal Title: J Toxicol Sci. 34 Pages: 445-48
  • NAID: 110007359651
  • Peer Reviewed
• [Journal Article] Nuclear changes in skeletal muscle extend to satellite cells in autosomal dominant Emerry-Dreifuss muscular dystrophy/lime-girdle muscular dystrophy 1B. (2009)
  • Author(s): Park YE, Hayashi YK, Goto K, Komaki H, Hayashi Y, et al.
  • Journal Title: Neuromuscul Disord 19 Pages: 29-36
  • Peer Reviewed
• [Journal Article] Exercise induces metallothioneins in mouse spinal cord. (2009)
  • Author(s): Hashimoto K, Hayashi Y, Inuzuka T, Hozumi I
  • Journal Title: Neuroscience 163 Pages: 244-251
  • Peer Reviewed
• [Journal Article] DNA microarray analysis of transcriptional responses of mouse spinal cords to physical exercise (2009)
  • Author(s): Hashimoto K, Honda A, Hayashi Y, Inuzuka T, Satoh M, Hozumi I
  • Journal Title: J Toxicol Sci. 34 Pages: 445-448
  • NAID: 110007359651
  • Peer Reviewed
• [Journal Article] Nuclear changes in skeletal muscle extend to satellite cells in autosomal dominant Emerry-Dreifuss muscular dystrophy/lime-girdle muscular dystrophy 1B. (2009)
  • Author(s): Park YE, Hayashi YK, Goto K, Komaki H, Hayashi Y, Inuzuka T, Noguchi S, Nonaka I, Nishino I
  • Journal Title: Neuromuscul Disord. 19 Pages: 29-36
  • Peer Reviewed
• [Journal Article] Nuclear changes in skeletal muscle extend to satellite cells in autosomal dominant Emerry-Dreifuss muscular dystrophy/lime-girdle muscular dystrophy 1B (2009)
  • Author(s): Park YE, Hayashi YK, Coto K, Komaki H, Hayashi Y, Inuzuka T, Noguchi S, Nonaka I, Nishino I.
  • Journal Title: Neuromuscul Disord 19 Pages: 29-36
  • Peer Reviewed
• [Journal Article] Progressive multifocal leukoencephalopathy and CD4+ T-lymphocytopenia in a patient with Sjogren syndrome. (2008)
  • Author(s): ayashi Y, et al.
  • Journal Title: J Neurol Sci. 268 Pages: 195-98
  • Peer Reviewed
• [Journal Article] Selective hypertrophy of cauda equina with idiopathic inflammation. (2008)
  • Author(s): Hayashi Y, et al.
  • Journal Title: Muscle Nerve 38 Pages: 1065-69
  • Peer Reviewed
• [Journal Article] Relationship between ribosomal RNA gene transcription activity and motoneuron death. (2008)
  • Author(s): Nagasao J, Hayashi Y, et al.
  • Journal Title: J Neurosci Res 86 Pages: 435-42
  • Peer Reviewed
• [Journal Article] Selective hypertrophy of cauda equina with idiopathic inflammation (2008)
  • Author(s): Hayashi Y, Sakurai T, Kimura A, Ikeda T, Matsuyama Z, Suzuki Y, Tanaka. Y, Hozumi I, Hosoe H, Takahashi T, Inuzuka T
  • Journal Title: Muscle and Nerve 38 Pages: 1065-1069
  • Peer Reviewed
• [Journal Article] Case report of a patient with Hashimoto's encephalopathy associated with Basedow's disease mimicking Creutzfeldt-Jakob disease (2008)
  • Author(s): Sakurai T, Tanaka Y, Koumura A, Hayashi Y, Kimura A, Hozumi I, Yoneda M, Inuzuka T.
  • Journal Title: Brain and Nerve 60 Pages: 559-565
  • Peer Reviewed
• [Journal Article] Progressive multifocal leukoencephalopathy and CD4+ T-lymphocytop enia in a patient with Sjogren syndrome (2008)
  • Author(s): Hayashi Y, Kimura A, Kato S, Koumura A, Sakurai T, Tanaka Y, Hozui I, Sunden Y, Orba Y, Sawa H, Takahashi H, Inuzuka T.
  • Journal Title: Journal of the Neurological Science. 268 Pages: 195-198
  • Peer Reviewed
• [Journal Article] Relationship between ribosomal RNA gene transcription activity and motoneuron death: Observations of avulsion and axotomy of the facial nerve in rats (2008)
  • Author(s): Nagasao J, Hayashi Y, Kawazoe Y, Kawakami E, Watabe K, Oyanagi K.
  • Journal Title: Journal of the Neurological Research. 86 Pages: 435-442
  • Peer Reviewed
• [Journal Article] Selective hypertrophy of cauda equina with idiopathic inflammation (2008)
  • Author(s): Hayashi Y, Sakurai T, Kimura A, Ikeda T, Matsuyama Z, Suzuki Y, Tanaka Y, Hozumi I, Hosoe H.Takahashi T, Inuzuka T.
  • Journal Title: Muscle and Nerve. (In press)
  • Peer Reviewed
• [Presentation] Growth inhibitory factor inhibits the progression of amyotrophic lateral sclerosis. (2010)
  • Author(s): Hashimoto K, Hayashi Y, Inuzuka T, Hozumi I
  • Organizer: Neurology 74 Suppl2
  • Place of Presentation: Canada
• [Presentation] 運動による脊髄メタロチオネインの誘導 (2008)
  • Author(s): 橋本和宣, 林祐一, ほか
  • Organizer: 第9回東海メタロチオネイン研究会
  • Place of Presentation: 岐阜
  • Year and Date: 2008-12-13
• [Presentation] 筋萎縮性側索硬化症に対する抗酸化物質と運動療法の効果 (2007)
  • Author(s): 林 祐一
  • Organizer: 平成19年度椿記念新潟神経内科研究会
  • Place of Presentation: 新潟大学
  • Year and Date: 2007-11-17