In vitro assessment of three-dimensional tissue engineered articular cartilage with a combination of quantitative MR imaging techniques

• Project/Area Number: 19790896
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Teikyo University
• Principal Investigator: WATANABE Atsuya Teikyo University, 医学部, 准教授 (40375712)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥570,000); Fiscal Year 2010: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 再生軟骨 / 間葉系幹細胞 / 三次元培養軟骨 / 磁気共鳴撮像 / 軟骨再生 / Magnetic resonance imaging

Research Abstract

In this study, we established an in vitro quantitative MR imaging technique of 3D tissue engineered cartilage with a combination of dGEMRIC, T2 mapping, and 3D SPGR MRI. Using the established MR imaging technique, we determined the optimal conditions for culturing 3D tissue engineered cartilage using rabbit mesenchymal stem cell, including seeding cell number, scaffold size, and in vitro culture duration.

Research Products

• [Presentation] Assessment of three-dimensional tissue engineered articular cartilage with a combination of quantitative MR imaging techniques. (2010)
  • Author(s): Atsuya Watanabe
  • Organizer: 第7回Combined Meeting of the Orthopaedic Research Societies
  • Place of Presentation: 日本、京都
  • Year and Date: 2010-10-16
• [Presentation] In vitro assessment of three dimensional tissue engineered articular cartnage with a combination of qualltitative MR imaging techniques (2010)
  • Author(s): 渡辺淳也
  • Organizer: 2009年度国際関節症学会
  • Place of Presentation: カナダ、モントリオール
  • Year and Date: 2010-09-10
• [Presentation] In vitro assessment of three-dimensional tissue engineered articular car tilage with a combination of quantitative MR imaging techniques. (2010)
  • Author(s): 渡辺淳也
  • Organizer: 2009年度国際関節症学会
  • Place of Presentation: カナダ、モントリオール
  • Year and Date: 2010-09-10
• [Presentation] Evaluation of three dimensional tissue engineered articular cartilage with a combination of quantitative MR imaging techniques (2010)
  • Author(s): 渡辺淳也
  • Organizer: 第8回国際軟骨再生学会
  • Place of Presentation: 米国、マイアミ
  • Year and Date: 2010-05-23
• [Presentation] Evaluation of three-dimensional tissue engineered articular cartilage with a combination of quantitative MR imaging techniques. (2010)
  • Author(s): 渡辺淳也
  • Organizer: 第8回国際軟骨再生学会
  • Place of Presentation: 米国、マイアミ
  • Year and Date: 2010-05-23
• [Presentation] In vitro assessment of three-dimensional tissue engineered articular cartilage with a combination of quantitative MR imaging techniques. (2010)
  • Author(s): Atsuya Watanabe
  • Organizer: The 7th Combined Meeting of the Orthopaedic Research Societies
  • Place of Presentation: Kyoto, Japan
• [Presentation] In vitro assessment of three dimensional tissue engineered articular cartilage with a combination of quantitative MR imaging techniques (2009)
  • Author(s): Atsuya Watanabe
  • Organizer: 2009年度Osteoarthritis Research Society International
  • Place of Presentation: カナダ、モントリオール
  • Year and Date: 2009-09-10
• [Presentation] Evaluation of three dimensional tissue engineered articular cartilage with a combination of quantitative MR imaging techniques (2009)
  • Author(s): Atsuya Watanabe
  • Organizer: 第8回International Cartilage Repair Society
  • Place of Presentation: 米国、マイアミ
  • Year and Date: 2009-05-23
• [Presentation] 三次元培養軟骨のMRIによる多角的組織組成評価 (2008)
  • Author(s): 渡辺淳也
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-10-23
• [Presentation] dGEMRIC及びT2マッピングを用いた三次元培養軟骨組織の多角的組織組成評価 (2008)
  • Author(s): 渡辺淳也
  • Organizer: 第36回日本磁気共鳴医学会大会
  • Place of Presentation: 旭川
  • Year and Date: 2008-09-12