Gene expressions associated to chemosensitivity in pancreatic cancer patients

• Project/Area Number: 19790959
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: KURAMOCHI Hidekazu Tokyo Women's Medical University, 医学部, 助教 (30287362)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥570,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 膵臓癌 / 化学療法 / S-1 / Gemcitabine / DNAマイクロアレイ

Research Abstract

We evaluated the gene expression levels associated with chemosentitivity in 81 pancreatic cancer patients after complete resection using real-time PCR. DPD, DHFR, FPGS, and GGH were found to be biomarkers of survival in those patients. We verified the function of those genes in vitro experiments.

Research Products

• [Journal Article] Epidermal growth factor receptor (EGFR) mRNA levels and protein expression levels in primary colorectal cancer and corresponding liver metastases. (2010)
  • Author(s): Kuramochi H, Hayashi K, Nakajima G, et al, 他4名、1番目
  • Journal Title: Cancer Chemother Pharmacol. 65(5) Pages: 825-31
  • Peer Reviewed
• [Journal Article] Epidermal growth factor receptor (EGFR) mRNA levels and protein expression levels in primary colorectal cancer and corresponding liver metastases. (2010)
  • Author(s): Kuramochi H, et al.
  • Journal Title: Cancer Chemotherapy and Pharmacology 65(5) Pages: 825-31
  • Peer Reviewed
• [Journal Article] Loss of heterozygosity at thymidylate synthase locus in Barrett's metaplasia, dysplasia, and carcinoma sequences. (2009)
  • Author(s): Kuramochi H, Uchida K, Peters JH, et al, 他5名、1番目
  • Journal Title: BMC Cancer. 9 Pages: 157-157
  • NAID: 120005517218
  • Peer Reviewed
• [Journal Article] Validation of a rodent model of Barrett's esophagus using quantitative gene expression profiling. (2009)
  • Author(s): Oh DS, DeMeester SR, Dunst CM,, Kuramochi H, et al, 他8名、6番目
  • Journal Title: Surg Endosc 23(6) Pages: 1346-52
  • Peer Reviewed
• [Journal Article] Loss of Heterozygosity at thymidylate Synthase locus in Barrett's adenoid, dysplasia, and carcinoma (2009)
  • Author(s): Kuramochi H, et al.
  • Journal Title: BMC Cancer 9 Pages: 157-157
  • Peer Reviewed
• [Journal Article] The analysis of CCR4+/CXCR3+ cell ratio in gastrointestinal cancer patients receiving chemotherapy or immunotherapy (2008)
  • Author(s): Sakuyama R, Aruga A, Kuramochi H, 他4名、3番
  • Journal Title: Gan To Kagaku Ryoho 35(12) Pages: 2259-61
  • Peer Reviewed
• [Journal Article] Three cases of stomatitis caused by chemotherapy for gastrointestinal cancer that responded well to lafutidine (2008)
  • Author(s): Uchida K, Hayashi K, Kuramochi H, et al, 他2名、3番目
  • Journal Title: Gan To Kagaku Ryoho 35(8) Pages: 1435-8
  • Peer Reviewed
• [Journal Article] ERCC1 codon 118 polymorphism is a useful prognostic marker in patients with pancreatic cancer treated with platinum-based chemotherapy. (2008)
  • Author(s): Kamikozuru H, Kuramochi H, Hayashi K, 他2名、2番目
  • Journal Title: Int J Oncol. 32(5) Pages: 1091-6
  • Peer Reviewed
• [Journal Article] High intratumoral dihydropyrimidine dehydrogenase. A levels in pancreatic cancer associated with a high rate of response to S-1. (2008)
  • Author(s): Kuramochi H, Hayashi K, Uchida K, et al, 他6名、1番目
  • Journal Title: Cancer Chemother Pharmacol. 63(1) Pages: 85-9
  • Peer Reviewed
• [Journal Article] Messenger RNA expression of vascular endothelial growth factor and its receptors in primary colorectal cancer and corresponding liver metastasis. (2008)
  • Author(s): Kobayashi H, Sugihara K, Kuramochi H, 他7名、6番目
  • Journal Title: Ann Surg Oncol 15(4) Pages: 1232-8
  • Peer Reviewed
• [Journal Article] Thymidylate synthase polymorphisms and mRNA expression are independent chemotherapy predictive markers in esophageal adenocarcinoma patients. (2008)
  • Author(s): Kuramochi H, Tanaka K, Oh D, et al, 他8名、1番目
  • Journal Title: Int J Oncol. 32(1) Pages: 201-8
  • Peer Reviewed
• [Journal Article] High intratumoral DPD mRNA levels in pancreatic cancer associated- (2008)
  • Author(s): Kuramochi H, et.al.
  • Journal Title: Cancer Chemotherapy and Pharmacology 63 Pages: 85-89
  • Peer Reviewed
• [Journal Article] High intratumoral DPD mRNA levels in pancreatic cancer associated〜 (2008)
  • Author(s): Kuramochi H, et. al.
  • Journal Title: Cancer Chemotherapy and Pharmacology (E pub)
  • Peer Reviewed
• [Journal Article] Thymidylate synthase polymorphisms and mRNA expression are〜 (2008)
  • Author(s): Kuramochi H, et. al.
  • Journal Title: International Journal of Oncology 32 Pages: 201-208
  • Peer Reviewed
• [Journal Article] Towards the molecular characterization of disease: comparison of molecular and histological analysis of esophageal epithelia. (2007)
  • Author(s): Vallbohmer D, Marjoram P, Kuramochi H, et al, 他9名、3番目
  • Journal Title: J Gastrointest Surg. 11(9) Pages: 1095-104
  • Peer Reviewed
• [Journal Article] DPD is a molecular determinant of capecitabine efficacy in colorectal cancer. (2007)
  • Author(s): Vallbohmer D, Yang DY, Kuramochi H, 他6名、3番目
  • Journal Title: Int J Oncol 31(2) Pages: 413-8
  • Peer Reviewed
• [Journal Article] Reduction of interleukin 8 gene expression in reflux esophagitis and Barrett's esophagus with antireflux surgery. (2007)
  • Author(s): Oh DS, DeMeester SR, Kuramochi H, 他8名、5番目
  • Journal Title: Arch Surg 142(6) Pages: 554-9
  • Peer Reviewed
• [Journal Article] Combination therapy of S-1 and CDDP for patients with colorectal cancer. (2007)
  • Author(s): Uchida K, Hayashi K, Kuramochi H, 他3名、3番目
  • Journal Title: J Cancer Res Clin Oncol 133(11) Pages: 841-6
  • Peer Reviewed
• [Journal Article] 肝内胆管癌に対する術後補助化学療法 (2007)
  • Author(s): 倉持 英和, 他
  • Journal Title: 肝胆膵 55 Pages: 915-918
• [Presentation] S-1+1-OHP療法を施行した大腸癌症例におけるERCC1多型の感受性マーカーとしての有用性 (2009)
  • Author(s): 倉持英和, 他
  • Organizer: 癌治療学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-23
• [Presentation] Correlation of folate-metabolizing enzymes with the outcomes of patients treated with gemcitabine or S-1 for adjuvant chemotherapy of advanced pancreatic cancer (2009)
  • Author(s): Ayako Nakamura, Hidekazu Kuramochi, et al.
  • Organizer: AACR
  • Place of Presentation: Denver,CO,USA
  • Year and Date: 2009-04-19
• [Presentation] S-1+l-OHP療法を施行した大腸癌症例におけるERCC1多型の感受性マーカーとしての有用性。 (2009)
  • Author(s): 倉持英和, ほか
  • Organizer: 第47回日本癌治療学会総会口演
  • Place of Presentation: 横浜
• [Presentation] Correlation of folate-metabolizing enzymes with the outcomes of patients treated with gemcitabine or S-1 for adjuvant chemotherapy of advanced pancreatic cancer. (2009)
  • Author(s): A. Nakamura, H. Kuramochi
  • Organizer: AACR meeting 2009
  • Place of Presentation: Denver, USA
• [Presentation] 進行再発大腸癌に対するS-1+オキサリプラチン併用療法の第1相試験 (2008)
  • Author(s): 倉持英和, 他
  • Organizer: 癌治療学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-31
• [Presentation] 進行再発大腸癌に対するS-1+オキサリプラチン併用療法(SOX療法)の第一相試験。 (2008)
  • Author(s): 倉持英和, ほか
  • Organizer: 第46回日本癌治療学会総会口演
  • Place of Presentation: 名古屋
• [Presentation] 大腸癌原発巣と肝転移巣におけるEGFRmRNA発現レベルと蛋白発現との関連性の検討。 (2008)
  • Author(s): 倉持英和, ほか
  • Organizer: 第63回日本消化器外科学会総会口演
  • Place of Presentation: 札幌
• [Presentation] Thymidylate Synthase (TS)遺伝子多型と遺伝子発現の食道腺癌患者における化学療法感受性マーカーとしての有用性の検討。 (2008)
  • Author(s): 倉持英和, ほか
  • Organizer: 第6回日本臨床腫瘍学会口演
  • Place of Presentation: 福岡
• [Presentation] 大腸癌原発巣と肝転移巣におけるEpidermal Growth Factor Receptor (EGFR)の遺伝子発現量の検討。 (2007)
  • Author(s): 倉持英和, ほか
  • Organizer: 第62回日本消化器外科学会総会口演
  • Place of Presentation: 東京
• [Presentation] Epidermal growth factor receptor (EGFR) mRNA expression levels are〜 (2007)
  • Author(s): Kuramochi H
  • Organizer: American Society of Clinical Oncology
  • Place of Presentation: Chicago
• [Presentation] Evaluation of thymidylate synthase and ERCC1 mRNA levels as predictive markers in colorectal cancer patients treated with S-1 and oxaliplatin.
  • Author(s): H. Kuramochi, K. Hayashi, G. Nakajima, H. Kamikozuru, M. Yamamoto.
  • Organizer: 2009 ASCO Annual Meeting Abstract No. 15071
  • Place of Presentation: Orlando, USA
• [Presentation] High intratumoral Dihydropyrimidine
  • Author(s): H. Kuramochi, K. Hayashi, H. Kamikozuru, G. Nakajima, T. Hatori, M. Yamamoto
  • Organizer: IPRF Forum 2009
  • Place of Presentation: Tokyo, Japan
• [Presentation] The relationship between epidermal growth factor receptor (EGFR) mRNA levels and protein expression in primary colorectal cancer and corresponding liver metastases.
  • Author(s): H. Kuramochi, K. Hayashi, G. Nakajima, K.D. Danenberg, P.V. Danenberg, M. Yamamoto
  • Organizer: 2008 ASCO Annual Meeting Abstract No. 15021
  • Place of Presentation: Chicago, USA
• [Presentation] Epidermal growth factor receptor (EGFR) mRNA expression levels are strongly correlated between primary colorectal cancer and corresponding liver metastases.
  • Author(s): H. Kuramochi, K. Hayashi, K. Uchida, M. Yamamoto, K.D. Danenberg, P.V. Danenberg
  • Organizer: 2007 ASCO Annual Meeting No. 4106
  • Place of Presentation: Chicago, USA