Middle ear regeneration of grafting of epithelial cell sheet

Research Project

Project/Area Number	19791239
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Otorhinolaryngology
Research Institution	Jikei University School of Medicine
Principal Investigator	YAMAMOTO Kazuhisa Jikei University School of Medicine, 医学部, 助教 (50408449)
Project Period (FY)	2007 – 2009
Project Status	Completed (Fiscal Year 2009)
Budget Amount *help	¥2,920,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥420,000) Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2007: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywords	中耳粘膜 / 粘膜再生 / 中耳真珠腫 / 温度応答性培養皿 / 粘膜移植 / 鼻粘膜 / 家兎
Research Abstract	Regeneration of the middle ear mucosa is important for a good prognosis after middle-ear surgery. It prevents renewed retraction of the tympanic membrane and recurrence of the cholesteatoma. To examine whether implantation of autologus mucosal epithelial cell sheet promotes mucosal regeneration, we reconstructed mucosal epithelial cell sheets using temperature-responsive culture dish and assessed its effectiveness for regeneration in middle ear-related diseases. In the animal study, the mucosal epithelial cell sheet grafting is effective for mucosal regeneration. These experimental findings suggest that the mucosal epithelial cell sheet grafting is as an effective treatment for achieving mucosal regeneration after middle ear surgery. The cell sheet might have the potential to be useful as graft material for post operative middle ear mucosal regeneration.