The role of Angiotensin II receptor subtypes in retinal ganglion cell

Research Project

Project/Area Number	19791276
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Ophthalmology
Research Institution	Ehime University
Principal Investigator	MIZOUE Shiro Ehime University, 大学院・医学系研究科, 講師 (80423458)
Project Period (FY)	2007 – 2009
Project Status	Completed (Fiscal Year 2009)
Budget Amount *help	¥2,750,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥450,000) Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2007: ¥800,000 (Direct Cost: ¥800,000)
Keywords	アンジオテンシンII受容体 / 網膜神経節細胞 / 緑内障 / アポトーシス / 動物モデル / アンジオテンシンII
Research Abstract	The AT1a angiotensin II receptor has been shown to be expressed in the retinal ganglion cells of wild type mice (C57BL/6J). Using intravitreal injection of NMDA, we were able to create a highly reproducible model of apoptosis in retinal ganglion cells. To examine the association between apoptosis and the angiotensin II system, we compared NMDA-induced retinal ganglion cell damage in AT1a (AT1aKO) and AT2 (AT2KO) receptor knock-out mice with that in wild type mice (C57BL/6J). No significant difference in ganglion cell count or inner plexiform layer (IPL) thickness was observed between the AT1aKO, AT2KO and wild type mice.