|Budget Amount *help
¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
The aim of this study was to elucidate the process of healing after transplantationed human ex vivo-produced oral mucosa equivalents by cryopreserved oral keratinocytes (frozen EVPOMEs). Normal EVPOMEs, by not cryopreserved oral keratinocytes, was used as a control. Two groups of EVPOMEs were transplanted into the back of nude mice. Mice were killed on days 1, 7, 14, 21, and 28 posttransplantation. The healing process of the transplanted EVPOMEs was evaluated with histologically. There were differences of the vessel density and of the epithelial stratification between two groups on 14 days posttransplantation. However, There were no difference between two groups on 28 days posttransplantation. The results of this study were indicated that two groups of EVPOMEs was same process of healing posttransplantation.