| Budget Amount *help |
¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000)
Fiscal Year 2022: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2021: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2020: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2019: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Outline of Final Research Achievements |
To innovate the translational function of E. coli, heterologous 23S rRNA gene was PCR amplified from the environmental metagenomes. The amplicon was incorporated into an expression vector, and E. coli strains deficient in the 23S rRNA gene were screened by means of genetic complementation as, and mutants with faster growth rates were accumulated. The 23S rRNA gene sequence was inserted at a homologous site with an intron containing a homing endonuclease (HE) in the 23S rRNA gene of Rhodothermus marinus, which was discovered previously, but failed to replace the E. coli 23S rRNA genes in the genome.
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