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Maintenance of diatom bloom by viruses

Research Project

Project/Area Number 19H00956
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
Research InstitutionFisheries Research and Education Agency

Principal Investigator

Tomaru Yuji  国立研究開発法人水産研究・教育機構, 水産技術研究所(廿日市), 主任研究員 (10416042)

Co-Investigator(Kenkyū-buntansha) 三木 健  龍谷大学, 先端理工学部, 教授 (00815508)
山口 晴生  高知大学, 教育研究部自然科学系農学部門, 准教授 (10432816)
本郷 悠貴  国立研究開発法人水産研究・教育機構, 水産資源研究所(横浜), 主任研究員 (20737316)
羽野 健志  国立研究開発法人水産研究・教育機構, 水産技術研究所(廿日市), 主任研究員 (30621057)
Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥45,110,000 (Direct Cost: ¥34,700,000、Indirect Cost: ¥10,410,000)
Fiscal Year 2023: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2022: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2021: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2020: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2019: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Keywords珪藻 / ウイルス / ゲノム / ssDNA / ssRNA / 連続培養 / ブルーム / 増殖速度 / 半連続培養 / 代謝産物 / 遺伝子発現
Outline of Research at the Start

珪藻は植物プランクトンの一種であり,その膨大な生産力は豊かな海洋生態系を支える原動力となっている。珪藻は,自身を殺すウイルスが存在しても長期の大増殖を維持することが可能である。その仕組みは未だ謎であるが,一つの仮説として珪藻はウイルス感染に抵抗する術を持ちつつも,分裂不調な細胞をウイルスで速やかに殺し,栄養塩として再利用する可能性が示唆されている。本課題では,海洋珪藻の全く新しい生態戦略「ウイルス感染死を介した珪藻のブルーム維持戦略」を提唱することを最終目的とする。

Outline of Final Research Achievements

We conducted an experiment to clarify the phenomenon in which diatoms, a type of phytoplankton that forms the foundation of marine ecosystems, are able to maintain their populations even in the presence of infectious viruses. The results revealed that the faster growing the diatom cells are less likely to be killed by virus infection, and the slower cells are likely to be killed. From the above, it was inferred that the slower growing cells are eliminated from the population by virus infection, while faster growing cells maintain the population.

Academic Significance and Societal Importance of the Research Achievements

珪藻が全地球上の海洋生態系ダイナミクスを理解する大きなカギになっていることが,近年の研究によって明らかにされつつある。ところが,何故,珪藻はウイルスが存在しても長期ブルームを維持出来るのか?についての全貌は十分に理解されているとは言い難い。我々の研究は,珪藻が分裂不調な細胞はウイルスで速やかに殺し→それら細胞遺骸を珪藻自身の強力な酵素で分解→栄養塩として再利用する,という一連の流れを示唆した。本研究成果に基づき,海洋珪藻の全く新しい生態戦略「ウイルス感染死を介した珪藻のブルーム維持戦略」という独創性の高い仮説が支持された。

Report

(7 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Comments on the Screening Results   Annual Research Report
  • Research Products

    (45 results)

All 2024 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (7 results) Journal Article (23 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 22 results,  Open Access: 18 results) Presentation (14 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (1 results)

  • [Int'l Joint Research] ウプサラ大学(スウェーデン)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] ウプサラ大学(スウェーデン)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] ウプサラ大学(スウェーデン)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] ウプサラ大学(スウェーデン)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] ヘルシンキ大学(フィンランド)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] ウプサラ大学(スウェーデン)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ヘルシンキ大学(フィンランド)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Transcriptional responses of the marine diatom Chaetoceros tenuissimus to phosphate deficiency2023

    • Author(s)
      Hongo Yuki、Hano Takeshi、Yamaguchi Haruo、Tomaru Yuji
    • Journal Title

      Gene

      Volume: 884 Pages: 147695-147695

    • DOI

      10.1016/j.gene.2023.147695

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of a simple method for cryopreservation of the marine diatoms, Chaetoceros and Phaeodactylum2023

    • Author(s)
      Yamaguchi Haruo、Sumida Narumi、Matsukawa Kazutsugu、Adachi Masao、Tomaru Yuji、Ishikawa Kosuke、Hotta Jun-ichi
    • Journal Title

      Journal of Applied Phycology

      Volume: 35 Issue: 5 Pages: 2285-2293

    • DOI

      10.1007/s10811-023-03040-w

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single-Cell Level Raman Molecular Profiling Reveals the Classification of Growth Phases of <i>Chaetoceros tenuissimus</i>2023

    • Author(s)
      Ando Masahiro、Sugiyama Kaori、Kubo Koya、Horii Shumpei、Hano Takeshi、Tomaru Yuji、Takeyama Haruko
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 127 Issue: 22 Pages: 5027-5033

    • DOI

      10.1021/acs.jpcb.3c02152

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chronological age-related metabolome responses in the dinoflagellate Karenia mikimotoi, can predict future bloom demise2023

    • Author(s)
      Hano Takeshi, Tomaru Yuji
    • Journal Title

      Communications Biology

      Volume: 6 Issue: 1

    • DOI

      10.1038/s42003-023-04646-z

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcriptional Responses of the Marine Planktonic Diatom <i>Chaetoceros tenuissimus</i> to Nitrogen- and Phosphorus-deficient States2022

    • Author(s)
      YAMAGUCHI Haruo、INOUE Minoru、TANIMOTO Yuko、SUMIDA Narumi、ADACHI Masao、KIMURA Kei、TOMARU Yuji
    • Journal Title

      Japan Agricultural Research Quarterly: JARQ

      Volume: 56 Issue: 3 Pages: 295-301

    • DOI

      10.6090/jarq.56.295

    • ISSN
      0021-3551, 2185-8896
    • Year and Date
      2022-07-01
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural Insights into Common and Host-Specific Receptor-Binding Mechanisms in Algal Picorna-like Viruses2022

    • Author(s)
      Wang H, Munke A, Tomaru T, Okamoto T
    • Journal Title

      Viruses

      Volume: 14 Issue: 11 Pages: 2369-2369

    • DOI

      10.3390/v14112369

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Primordial Capsid and Spooled ssDNA Genome Structures Unravel Ancestral Events of Eukaryotic Viruses2022

    • Author(s)
      Munke Anna、Kimura Kei、Tomaru Yuji、Wang Han、Yoshida Kazuhiro、Mito Seiya、Hongo Yuki、Okamoto Kenta
    • Journal Title

      mBio

      Volume: 13 Issue: 4

    • DOI

      10.1128/mbio.00156-22

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection and Characterization of RNA Viruses in Red Macroalgae (Bangiaceae) and Their Food Product (Nori Sheets)2022

    • Author(s)
      Mizutani Yukino、Chiba Yuto、Urayama Syun-ichi、Tomaru Yuji、Hagiwara Daisuke、Kimura Kei
    • Journal Title

      Microbes and Environments

      Volume: 37 Issue: 5 Pages: n/a

    • DOI

      10.1264/jsme2.ME21084

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ICTV Virus Taxonomy Profile: Marnaviridae 20212021

    • Author(s)
      Lang Andrew S.、Vlok Marli、Culley Alexander I.、Suttle Curtis A.、Takao Yoshitake、Tomaru Yuji、ICTV Report Consortium
    • Journal Title

      Journal of General Virology

      Volume: 102 Issue: 8 Pages: 001633-001633

    • DOI

      10.1099/jgv.0.001633

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The genome of the diatom Chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus2021

    • Author(s)
      Hongo Yuki、Kimura Kei、Takaki Yoshihiro、Yoshida Yukari、Baba Shuichiro、Kobayashi Genta、Nagasaki Keizo、Hano Takeshi、Tomaru Yuji
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 22877-22877

    • DOI

      10.1038/s41598-021-00565-3

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 藻類ゲノムに伝播したウイルス様遺伝子とその進化的役割2021

    • Author(s)
      本郷悠貴、外丸裕司
    • Journal Title

      日本藻類学会誌

      Volume: 69 Pages: 160-164

    • NAID

      40022749758

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA Viruses in Aquatic Unicellular Eukaryotes.2021

    • Author(s)
      Sadeghi M, Tomaru Y, Ahola T
    • Journal Title

      Viruses

      Volume: 13 Issue: 3 Pages: 362-362

    • DOI

      10.3390/v13030362

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Growth Rate-dependent Cell Death of Diatoms due to Viral Infection and Their Subsequent Coexistence in a Semi-continuous Culture System2021

    • Author(s)
      Tomaru Y, Yamaguchi H, Miki T
    • Journal Title

      Microbes and Environments

      Volume: 36 Issue: 1 Pages: n/a

    • DOI

      10.1264/jsme2.ME20116

    • NAID

      130007964998

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chronological distribution of dino_agellate-infecting RNA virus in marine sediment core.2021

    • Author(s)
      Takahashi, M, Wada, K, Takano, Y, Matsuno, K, Masuda, Y, Arai, K, Murayama, M, Tomaru, Y, Nagasaki, K
    • Journal Title

      Sci Total Env

      Volume: 770 Pages: 20-20

    • DOI

      10.1016/j.scitotenv.2021.145220

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The possibility of using marine diatom-infecting viral promoters for the engineering of marine diatoms.2020

    • Author(s)
      Kadono T, Tomaru Y, Suzuki K, Yamada K, Adachi M
    • Journal Title

      Plant Science

      Volume: - Pages: 110475-110475

    • DOI

      10.1016/j.plantsci.2020.110475

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Viral RNA Genomes Identified from Marine Macroalgae and a Diatom2020

    • Author(s)
      Chiba Yuto、Tomaru Yuji、Shimabukuro Hiromori、Kimura Kei、Hirai Miho、Takaki Yoshihiro、Hagiwara Daisuke、Nunoura Takuro、Urayama Syun-ichi
    • Journal Title

      Microbes and Environments

      Volume: 35 Issue: 3 Pages: n/a

    • DOI

      10.1264/jsme2.ME20016

    • NAID

      130007855486

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel Protocol for Estimating Viruses Specifically Infecting the Marine Planktonic Diatoms.2020

    • Author(s)
      Tomaru Y, Kimura K
    • Journal Title

      Diversity

      Volume: 12 Issue: 6 Pages: 225-225

    • DOI

      10.3390/d12060225

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Previously unknown ssDNA molecules coccurring with CdebDNAV infecting the marine planktonic diatom Chaetoceros debilis2020

    • Author(s)
      Tomaru Y, Toyoda K, Kimura K
    • Journal Title

      Phycological Research

      Volume: 68 Issue: 4 Pages: 269-276

    • DOI

      10.1111/pre.12435

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Capsid structure of a marine algal virus of the order Picornavirales.2020

    • Author(s)
      Munke A, Kimura K, Tomaru Y, Okamoto K
    • Journal Title

      J Virol

      Volume: 94 Issue: 9 Pages: 1-20

    • DOI

      10.1128/jvi.01855-19

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Preliminary Analysis of Diatom-infecting Viruses in Ariake Sound, Japan2019

    • Author(s)
      Tomaru Y, Matsubara T, Mine T, Shikata T, Nagasaki K, Kimura K, Yamaguchi H
    • Journal Title

      Japan Agricultural Research Quarterly: JARQ

      Volume: 53 Issue: 3 Pages: 223-228

    • DOI

      10.6090/jarq.53.223

    • NAID

      130007706259

    • ISSN
      0021-3551, 2185-8896
    • Year and Date
      2019-07-01
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Metabolomics-based approach to explore growth phase-dependent markers in cultured diatom Chaetoceros tenuissimus2019

    • Author(s)
      Hano T, Tomaru Y
    • Journal Title

      Journal of Chromatography B

      Volume: 1128 Pages: 121779-121779

    • DOI

      10.1016/j.jchromb.2019.121779

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel marine diatom ssRNA virus NitRevRNAV infecting Nitzschia reversa.2019

    • Author(s)
      Toyoda K, Kimura K, Osada K, Williams DM, Adachi T, Yamada K, Tomaru Y
    • Journal Title

      Plant Ecology and Evolution

      Volume: 152 Issue: 2 Pages: 178-187

    • DOI

      10.5091/plecevo.2019.1615

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] プランクトン群集における共生・寄生 珪藻ウイルス研究のこれまでとこれから2019

    • Author(s)
      外丸裕司
    • Journal Title

      海洋と生物

      Volume: 41 Pages: 301-305

    • Related Report
      2019 Annual Research Report
  • [Presentation] 海産珪藻Chaetoceros tenuissimusのウイルス感染時の遺伝子発現2024

    • Author(s)
      本郷悠貴、羽野健志、外丸裕司
    • Organizer
      日本藻類学会2024
    • Related Report
      2023 Annual Research Report
  • [Presentation] 珪藻に感染するDNAウイルスの感染特性から評価と感染機構の理解2024

    • Author(s)
      前田伊央莉、吉田和広、外丸裕司、木村圭
    • Organizer
      日本藻類学会2024
    • Related Report
      2023 Annual Research Report
  • [Presentation] 植物プランクトンに感染するウイルスの研究~水産学的な役割を探る~2024

    • Author(s)
      外丸裕司
    • Organizer
      農芸化学会中四国支部大会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 浦ノ内湾における珪藻Chaetoceros tenuissimusおよび同藻感染ウイルスの諸性状2023

    • Author(s)
      山本脩生、外丸裕司、山口晴生
    • Organizer
      土佐湾生物学会2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] Diatom viruses isolated from coastal waters and sediments in western Japan2023

    • Author(s)
      Tomaru Yuji
    • Organizer
      ICHA20
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 珪藻RNAウイルスの感染特異性はウイルス吸着過程に依存するのか?2022

    • Author(s)
      水戸誠也、外丸裕司、木村圭
    • Organizer
      令和4年度日本プランクトン学会ベントス学会合同大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 新たに分離したChaetoceros tenuissimus感染ウイルスクローンの殺藻特性に基づいた同ウイルスー宿主珪藻の生態学的関係2022

    • Author(s)
      佐藤立都、山口晴生、外丸裕司
    • Organizer
      令和4年度日本プランクトン学会ベントス学会合同大会講演要旨集
    • Related Report
      2022 Annual Research Report
  • [Presentation] 海産珪藻Chaetoceros tenuissimusのゲノム中に見られた感染性ウイルス遺伝子の挿入履歴2022

    • Author(s)
      本郷悠貴、羽野健志、外丸裕司、他
    • Organizer
      日本藻類学会第46回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 珪藻と珪藻ウイルスに関する生理生態学的研究Ⅰ.宿主殺藻に及ぼす栄養塩の影響2021

    • Author(s)
      佐藤立都、山口晴生、外丸裕司
    • Organizer
      令和3年度日本水産学会秋季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Integration of ssDNA fragment from an infectious virus in the genome of the diatom Chaetoceros tenuissimus.2021

    • Author(s)
      本郷悠貴、外丸裕司、他
    • Organizer
      Aquatic Virus Workshop 10
    • Related Report
      2021 Annual Research Report
  • [Presentation] 海の牧草を持続的に管理するウイルス2021

    • Author(s)
      外丸裕司
    • Organizer
      高知大学 研究拠点プロジェクト2020年度 公開シンポジウム海の恵みを享受するために
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 珪藻感染性DNAウイルスゲノムに存在する 機能不明遺伝子の発現2019

    • Author(s)
      外丸裕司
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Challenges for detecting diverse viruses infecting marine planktonic diatoms from coastal sediments2019

    • Author(s)
      Tomaru Yuji
    • Organizer
      EastHAB2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 本邦沿岸底泥における珪藻Chaetoceros tenuissimusの分布2019

    • Author(s)
      角田成美
    • Organizer
      令和元年度日本水産学会秋季大会
    • Related Report
      2019 Annual Research Report
  • [Book] 理科年表2020

    • Author(s)
      外丸裕司、長崎慶三
    • Total Pages
      1204
    • Publisher
      丸善出版
    • ISBN
      9784621305607
    • Related Report
      2020 Annual Research Report

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Published: 2019-04-18   Modified: 2025-01-30  

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