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High-quality single crystal growth of DNA-functionalized nanoparticles

Research Project

Project/Area Number 19H02555
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 28030:Nanomaterials-related
Research InstitutionNagoya University

Principal Investigator

Tagawa Miho  名古屋大学, 未来材料・システム研究所, 教授 (40512330)

Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2023: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2022: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2021: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2020: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
KeywordsDNA / ナノ粒子 / 結晶成長 / X線小角散乱 / 自己集合 / コロイド結晶成長 / 自己組織化 / Self-assembly / 機能性ナノ材料
Outline of Research at the Start

本研究では下記課題1-3に取り組み、DNA-NP超格子の単結晶成長法の確立とサイズ向上を目指す。
課題1:核生成頻度を下げるため、一旦作製したDNA-NP超格子結晶を種結晶として再度結晶化溶液に入れ、成長させる
課題2:結晶を吊り下げて成長させる
課題3:ポリエチレングリコール(PEG)による枯渇引力を利用した結晶成長
成長させるDNA-NP超格子結晶としては、二元系(互いに相補的なDNAペア)で粒子サイズが同じ場合にできるbcc構造、二元系で粒子サイズが異なる場合にできるCsCl構造、一元系(自己相補的なDNAペア)のfcc構造に取り組む。更に、DNA-NP超格子結晶のX線単結晶解析に取り組む。

Outline of Final Research Achievements

In the crystallization of DNA-functionalized nanoparticles (DNA-NPs), in which synthetic DNA is modified on the surface of metal nanoparticles, a new crystal growth model was developed by considering both colloidal crystal growth and DNA self-assembly, which have been treated as different research fields, and a high-quality single crystal growth method was successfully established. We also developed a new method for the growth of high quality single crystals. In addition, by combining small-angle X-ray scattering and rotational crystal methods, we developed a nano- to mesoscale single-crystal structure analysis method and succeeded in analyzing the internal structure of a single crystal.

Academic Significance and Societal Importance of the Research Achievements

DNAの選択的結合性を利用してナノ粒子を結晶化すると、ナノ粒子種の組み合わせや結晶構造、粒子間距離を自由自在に制御でき、構造自由度の高いナノ粒子超格子が作製できる。しかし、特性の良い物性を発現するための「結晶性」との両立が難しく、また単結晶を大きく成長させることも難しかった。本研究により、バルク材料(金塊等の巨視的な材料)とは異なる新奇な物性を持つナノ粒子が、更に周期構造化して発現する新奇な物性を見出し、応用する道が拓けた。

Report

(3 results)
  • 2023 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (12 results)

All 2021 2019

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Two-step nanoparticle crystallization via DNA-guided self-assembly and non-equilibrium dehydration process2021

    • Author(s)
      Hayato Sumi, Noboru Ohta, Hiroshi Sekiguchi, Shunta Harada, Toru Ujihara, Katsuo Tsukamoto, Miho Tagawa
    • Journal Title

      Crystal Growth & Design

      Volume: 21 Issue: 8 Pages: 4506-4515

    • DOI

      10.1021/acs.cgd.1c00398

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Optimization of crystallization conditions for the enlargement of the size ofDNA-functionalized nanoparticles crystals2021

    • Author(s)
      Lidong Zhang, Xinyi Zhou, Hayato Sumi, Noboru Ohta, Hiroshi Sekiguchi, Shunta Harada, Toru Ujihara, Miho Tagawa
    • Organizer
      ICMaSS2021(International Conference on Materials and Systems for Sustainability)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DNA-guided crystallization of nanoparticles: the effect of solvent composition on crystal structure2021

    • Author(s)
      Miho Tagawa, Maasa Yokomori, Yuji Maeda, Hayato Sumi, Noboru Ohta, Hiroshi Sekiguchi, Shunta Harada, Toru Ujihara
    • Organizer
      ICMaSS2021(International Conference on Materials and Systems for Sustainability)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DNA修飾ナノ粒子超格子の結晶サイズ向上のための成長条件最適化2021

    • Author(s)
      張 力東、横森 真麻、周 幸儀、鷲見 隼人、太田 昇、関口 博史、原田 俊太、宇治原 徹、田川 美穂
    • Organizer
      第82回応用物理学会秋季学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] DNA-guided crystallization of nanoparticles: design strategy of high-crystallinity nanoparticle superlattice2021

    • Author(s)
      田川美穂
    • Organizer
      2021 年度 ナノ構造・物性-ナノ機能・応用部会 合同シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] THE OPTIMUM DESIGN OF DNA-GUIDED NANOPARTICLE SUPERLATTICES FOR DIRECT DEHYDRATION2019

    • Author(s)
      H. Sumi, T. Isogai, S. Kojima, N. Ohta, H. Sekiguchi, S. Harada, T. Ujihara, M. Tagawa
    • Organizer
      the 19th International Conference on Crystal Growth and Epitaxy (ICCGE-19)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DNA-guided crystallization of nanoparticles: optimization of crystallization conditions and structure analysis2019

    • Author(s)
      Miho Tagawa, Shoko Kojima, Hayato Sumi, Noboru Ohta, Hiroshi Sekiguchi, Shunta Harada, Toru Ujihara
    • Organizer
      International Conference on Materials and Systems for Sustainability (ICMaSS) 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structural stability analysis of DNA-guided nanoparticle superlattice by direct dehydration2019

    • Author(s)
      Hayato Sumi, Noboru Ohta, Hiroshi Sekiguchi, Shunta Harada, Toru Ujihara, Miho Tagawa
    • Organizer
      International Conference on Materials and Systems for Sustainability (ICMaSS) 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of polyethylene glycol induced depletionattraction on DNA-functionalized nanoparticle crystalization2019

    • Author(s)
      Shoko Kojima, Hayato Sumi, Noboru Ohta, Hiroshi Sekiguchi, Shunta Harada, Toru Ujihara,Miho Tagawa
    • Organizer
      Okinawa Colloids 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] X線小角散乱法と回転結晶法を用いたコロイド単結晶中 の格子乱れの解析2019

    • Author(s)
      鷲見 隼人, 太田 昇, 関口 博史, 原田 俊太, 宇治原 徹, 田川 美穂
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] DNAガイドのナノ粒子結晶化:結晶化条件の最適化と構造解析2019

    • Author(s)
      田川 美穂、小島 憧子、鷲見 隼人、西部 愛里紗、磯貝 卓巳、横森 真麻、原田 俊太、宇治原 徹、塚本 勝男
    • Organizer
      日本地球惑星科学連合(JpGU2019)
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Patent(Industrial Property Rights)] ナノ粒子結晶及びその製造方法2019

    • Inventor(s)
      田川美穂、横森真麻
    • Industrial Property Rights Holder
      名古屋大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-169737
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

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Published: 2019-04-18   Modified: 2025-01-30  

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