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Genetic improvement of root system architecture which is robust for soil nutrient and water stresses in rice

Research Project

Project/Area Number 19H02936
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39010:Science in plant genetics and breeding-related
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Uga Yusaku  国立研究開発法人農業・食品産業技術総合研究機構, 作物研究部門, グループ長 (00391566)

Co-Investigator(Kenkyū-buntansha) 井上 晴彦  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (10435612)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2021: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords根系形態 / 環境ストレス / 伝子発現 / 重力屈性 / 水分屈性 / QTL / 環境ストレス耐性 / 水屈性 / 作物開発 / イネ / 作物 / 屈性 / 根系 / シグナル応答 / ゲノム編集 / 可視化
Outline of Research at the Start

不良環境下で作物生産を高く維持するには、環境ストレスに適応した根系を作出する必要がある。しかし、環境ストレスにより、場合によっては、根系は理想の形とは逆の方向に変化することがあり、不良土壌に適応した根系の状態に維持することは困難である。なぜなら、環境ストレスシグナルが根系関連遺伝子の働きに影響するからである。本研究では、環境ストレスシグナル応答経路と根系形態の制御経路に関する遺伝子の改変を通して、環境ストレスに適応的な作物根系を作出することをめざす。本成果によって環境ストレスに適応的な作物根系を作出するための新たな遺伝子リソースと基盤技術の確立が進むことが期待できる。

Outline of Final Research Achievements

Genetic improvement of the root system in crops to adapt to environmental stresses is important for stable crop production. In this study, we attempted to identify genes that act upstream or downstream of the rice QTL for root growth angle (qSOR1). Using rice lines with different alleles of qSOR1, we found that gene expression levels of several heat shock transcription factors (HSFs) at root tips varied with different environmental stimuli such as gravity change and drought stress. Since we speculated that these HSFs are likely to have some roles in both gravitropic and hydrotropic responses, we developed a knockout line by genome editing. In the future, we will clarify the effects of these genes on the regulation of root growth angle.

Academic Significance and Societal Importance of the Research Achievements

イネ根伸長角度QTL(qSOR1)の相同遺伝子は、単子葉植物だけでなく、双子葉植物にも広く存在する。そのため、qSOR1周辺の遺伝子群の同定は、陸上植物の環境適応における根張りの役割を解明するうえで重要な知見を提供する。これまで根系形態が環境ストレス耐性に貢献することが分かっていたが、遺伝的改良は困難であった。本知見は、根系形態を育種対象として扱ううえで、貴重な情報を提供するものであり、今後不安定化する地球規模の農地劣化に対応した農作物の品種改良への寄与が期待できる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (13 results)

All 2021 2020 2019 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (9 results) (of which Int'l Joint Research: 5 results,  Invited: 5 results) Remarks (1 results)

  • [Journal Article] Challenges to design-oriented breeding of root system architecture adapted to climate change2021

    • Author(s)
      Uga Yusaku
    • Journal Title

      Breeding Science

      Volume: 71 Issue: 1 Pages: 3-12

    • DOI

      10.1270/jsbbs.20118

    • NAID

      130007991330

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Root angle modifications by theDRO1homolog improve rice yields in saline paddy fields2020

    • Author(s)
      Kitomi Yuka、Hanzawa Eiko、Kuya Noriyuki、Inoue Haruhiko、Hara Naho、Kawai Sawako、Kanno Noriko、Endo Masaki、Sugimoto Kazuhiko、Yamazaki Toshimasa、Sakamoto Shingo、Sentoku Naoki、Wu Jianzhong、Kanno Hitoshi、Mitsuda Nobutaka、Toriyama Kinya、Sato Tadashi、Uga Yusaku
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 117 Issue: 35 Pages: 21242-21250

    • DOI

      10.1073/pnas.2005911117

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The intersection of nitrogen nutrition and water use in plants: new paths toward improved crop productivity2020

    • Author(s)
      Plett Darren C、Ranathunge Kosala、Melino Vanessa J、Kuya Noriyuki、Uga Yusaku、Kronzucker Herbert J
    • Journal Title

      Journal of Experimental Botany

      Volume: 71 Issue: 15 Pages: 4452-4468

    • DOI

      10.1093/jxb/eraa049

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Challenges to design-oriented breeding of root system architecture adapted to abiotic stress2021

    • Author(s)
      宇賀優作
    • Organizer
      11th Symposium of the International Society of Root Research and Rooting 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Comprehensive search for gravity-responsive genes of rice root tip after simulated microgravity condition generated by a three-dimensional clinostat2021

    • Author(s)
      久家徳之, 木富悠花, 西嶋遼, 川勝泰二, 宇賀優作
    • Organizer
      11th Symposium of the International Society of Root Research and Rooting 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Towards designed genetic improvement of root system architecture for developing of climate-resilient rice2021

    • Author(s)
      宇賀優作
    • Organizer
      10th Asian Crop Science Association Conference
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 3D クリノスタットによる微小重力処理後のイネ根端における重力刺激応答遺伝子の網羅的探索2021

    • Author(s)
      久家徳之, 木富悠花, 西嶋遼, 川勝泰二, 宇賀優作
    • Organizer
      日本育種学会第139回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 地表根形成遺伝子qSOR1はイネの根系形態を制御する重要な遺伝子ファミリーに属する2020

    • Author(s)
      宇賀優作, 木富悠花, 半澤栄子, 久家徳之, 井上晴彦, 原奈穂, 河合佐和子, 菅野徳子, 遠藤真咲, 杉本和彦, 山崎俊正, 坂本真吾, 千徳直樹, 呉健忠, 光田展隆, 佐藤雅志
    • Organizer
      日本育種学会第138回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Towards genetic improvement of root system architecture for developing of climate-resilient rice2019

    • Author(s)
      宇賀優作
    • Organizer
      2019 KSBS & SABRAO International Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] イネの根の形はDRO1 ファミリー遺伝子によってデザインすることができる2019

    • Author(s)
      木富悠花, 原奈穂・半澤栄子, 河合佐和子, 菅野徳子, 藤澤弘子, 金森裕之, 呉健忠, 佐藤雅志, 宇賀優作
    • Organizer
      イネ遺伝学・分子生物学ワークショップ2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] Desired control of root system architecture to develop climate-resilient rice2019

    • Author(s)
      宇賀優作
    • Organizer
      6th International Symposium on Genomics and Crop Genetic Improvement - Molecular Breeding
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 干ばつ耐性向上をめざした稲の根系改良の試みと今後の展望2019

    • Author(s)
      宇賀優作
    • Organizer
      日本熱帯農業学会第126回講演会公開シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Remarks] (研究成果) 世界初、根の改良により塩害に強いイネを開発

    • URL

      https://www.naro.go.jp/publicity_report/press/laboratory/nics/136355.html

    • Related Report
      2020 Annual Research Report

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Published: 2019-04-18   Modified: 2023-01-30  

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