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Study on reproductive mechanism in grouper by ecophysiological approach

Research Project

Project/Area Number 19H03034
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40030:Aquatic bioproduction science-related
Research InstitutionNagasaki University

Principal Investigator

Soyano Kiyoshi  長崎大学, 海洋未来イノベーション機構, 教授 (80260735)

Co-Investigator(Kenkyū-buntansha) 莚平 裕次  長崎大学, 海洋未来イノベーション機構, 特任研究員 (20806971)
村田 良介  長崎大学, 海洋未来イノベーション機構, 助教 (40809159)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2020: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2019: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywords繁殖メカニズム / 産卵行動 / 産卵回遊 / 成熟関連ホルモン / ハタ類 / 産卵関連行動 / 成熟誘起ホルモン
Outline of Research at the Start

重要水産対象種であるハタ類の天然資源を末永く利用するためには、次の世代を残すための繁殖のメカニズム(再生産機構)を解明し、ハタ類の資源管理とその生息環境を守る必要がある。本研究では、カンモンハタをモデル種として、天然海域におけるハタ類の繁殖特性と再生産機構を行動生理学・繁殖生理生態学的研究によって解明する。具体的には、魚に発信器を装着し、そこから出される音波を利用して行動を解析する。また、生殖腺の発達を統御するホルモン(体内の情報伝達物質)の遺伝子の発現を解析する。さらに、天然海域における月周期・潮汐・水温などの環境と行動や体内の変化を関連づけ、カンモンハタの繁殖のメカニズムを理解する。

Outline of Final Research Achievements

In order to establish a basis for resource management and development of aquaculture technology for grouper, an important fishery resource in Japan, we conducted an experiment using the honeycomb groupers that inhabit the coral reefs of Okinawa to clarify the relationship between spawning behavior and gonad development in natural waters. We attached a transmitter to a grouper captured in its habitat on the coral reef and re-released it to observe its migration between the habitat and the spawning grounds outside the reef. The results showed that the males migrated first to the spawning grounds and then the females migrated to the spawning grounds. After completing spawning, both males and females were found to return to their original habitat. In addition, the secretion of hormones which regulate gonadal development increased rapidly at the spawning grounds. This was shown to activate the physiological mechanisms for spawning.

Academic Significance and Societal Importance of the Research Achievements

本研究は天然フィールドにおいて捕獲した魚に発信機を装着し、海に設置した受信機でその行動を記録するバイオテレメトリー法を用いて産卵関連行動を調べた数少ない研究である。この研究において、産卵場への移動のタイミングが雄雌で違うことが明らかとなった。また、産卵移動前後の個体を捕獲し、生殖腺の発達を制御するホルモンを測定したところ、産卵場に到達した魚で排卵・産卵を引き起こすホルモンが増加することが分かった。このように産卵場への移動が産卵のための生理機構の活性化には必要であることを初めて明らかにした。これらの知見はハタ類の天然海域における再生産と資源管理に向けた重要な情報である。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (8 results)

All 2022 2021 2020 2019

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Endocrine regulation of maturation and sex change in groupers2022

    • Author(s)
      Kiyoshi Soyano, Takafumi Amagai, Tomofumi Yamaguchi, Yuji Mushirobira, Wen-Gang Xu, Nhan Thanh Pham, Ryosuke Murata
    • Journal Title

      Cells

      Volume: 11 Issue: 5 Pages: 825-825

    • DOI

      10.3390/cells11050825

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Male pheromones induce ovulation in female honeycomb groupers (Epinephelus merra): A comprehensive study of spawning aggregation behavior and Ovarian Development2022

    • Author(s)
      Takafumi Amagai, Daisuke Izumida, Ryosuke Murata, Kiyoshi Soyano
    • Journal Title

      Cells

      Volume: 11 Issue: 3 Pages: 484-484

    • DOI

      10.3390/cells11030484

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Testicular inducing steroidogenic cells trigger sex change in groupers2021

    • Author(s)
      Murata Ryosuke、Nozu Ryo、Mushirobira Yuji、Amagai Takafumi、Fushimi Jun、Kobayashi Yasuhisa、Soyano Kiyoshi、Nagahama Yoshitaka、Nakamura Masaru
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 11117-11117

    • DOI

      10.1038/s41598-021-90691-9

    • NAID

      120007166819

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spawning characteristics of a lunar-synchronized spawner, the honeycomb grouper Epinephelus merra, under artificial conditions.2020

    • Author(s)
      Amagai, T., Murata, R., Soyano, K.
    • Journal Title

      Aquaculture Science

      Volume: 68 Pages: 235-242

    • NAID

      130008090535

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a giant grouper Luteinizing Hormone (LH) Enzyme-Linked Immunosorbent Assay (ELISA) and its use towards understanding sexual development in grouper.2020

    • Author(s)
      Dennis, L., P., Nocillado, J., Palma, P., Amagai, T., Soyano, K., Elizur, A.
    • Journal Title

      General and Comparative Endocrinology

      Volume: 296 Pages: 113542-113542

    • DOI

      10.1016/j.ygcen.2020.113542

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] 天然海域におけるカンモンハタの産卵関連行動とそれに伴う繁殖生理変化2022

    • Author(s)
      池原宏紀・天谷貴史・征矢野清
    • Organizer
      日本水産学会九州支部大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 天然海域における月周産卵魚カンモンハタの産卵特性2019

    • Author(s)
      天谷貴史・泉田大介・征矢野清
    • Organizer
      令和元年度日本水産学会秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Spawning characteristics of the honeycomb grouper Epinephelus merra2019

    • Author(s)
      Amagai, T., Izumida, D., Soyano, K.
    • Organizer
      The 16th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2023-01-30  

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