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Elucidation of the molecular mechanisms that give rise to different neural subtypes by single cell transcriptomics

Research Project

Project/Area Number 19H03204
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 43050:Genome biology-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

Takeo Horie  筑波大学, 生命環境系, 助教 (10455925)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2021: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2020: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywordsホヤ / 単一細胞トランスクリプトーム解析 / 神経細胞 / 細胞分化 / 単一細胞トランスクリプトーム / シングルセルトランスクリプトーム / 遺伝子発現解析 / 神経発生
Outline of Research at the Start

本研究は、シンプルなホヤ幼生の中枢神経系をモデルとして、単一細胞トランスクリプトーム解析などのゲノム生物学的な手法と発生生物学的な手法を組み合わせことにより、中枢神経系に存在する全てのニューロンについて、異なる神経サブタイプを生み出す分子機構を解明する。そして、ホヤにおける知見を他のモデル動物の知見と比較し、進化的に保存された普遍的なニューロンの分化機構の解明を目指す。

Outline of Final Research Achievements

We study the ascidian simple CNS as a model system to elucidate the molecular mechanisms that give rise to different neural subtypes for all neurons in the central nervous system by combining genomic and developmental biological methods, such as single-cell transcriptomics. Our goal is to elucidate the molecular mechanisms that give rise to different neuronal subtypes for all neurons in the CNS.
From the single-cell transcriptomics data, we have elucidated the differentiation mechanisms of dopaminergic neurons, various types of GABAergic neurons, and glutamatergic epidermal sensory neurons. We also performed a comparative analysis of the differentiation mechanisms of sensory neurons in multiple animals to elucidate the differentiation mechanisms of sensory neurons that are conserved between left and right homologous animals.

Academic Significance and Societal Importance of the Research Achievements

本研究では、シンプルな神経系を持つホヤを用いることで様々な神経細胞の分化機構を解明した。さらに、解明した分化機構をもとに様々な神経細胞を人為的に作り出すことにも成功している。今後、これらの研究成果をマウスやヒトなど高等脊椎動物でも検証することにより、iPS細胞やES細胞から人為的に任意の神経細胞の分化を誘導する技術の開発へとつなげたい。そして、損傷した神経回路の修復など医学方面への応用を視野に入れた研究へと発展することが期待される。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (24 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (6 results) Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Open Access: 6 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (2 results)

  • [Int'l Joint Research] プリンストン大学(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] 北京師範大学(中国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] プリンストン大学(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] 北京師範大学(中国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] プリンストン大学(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] 北京師範大学(中国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] d-Serine controls epidermal vesicle release via NMDA receptor, allowing tissue migration during the metamorphosis of the chordate <i>Ciona</i>2022

    • Author(s)
      Krasovec Gabriel、Hozumi Akiko、Yoshida Tomoyuki、Obita Takayuki、Hamada Mayuko、Shiraishi Akira、Satake Honoo、Horie Takeo、Mori Hisashi、Sasakura Yasunori
    • Journal Title

      Science Advances

      Volume: 8 Issue: 10 Pages: 1-22

    • DOI

      10.1126/sciadv.abn3264

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Neuronal identities derived by misexpression of the POU IV sensory determinant in a protovertebrate2022

    • Author(s)
      Prakriti Paul Chacha, Ryoko Horie, Takehiro G. Kusakabe, Yasunori Sasakura, Mona Singh, Takeo Horie, and Michael Levine
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 119 Issue: 4

    • DOI

      10.1073/pnas.2118817119

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A single motor neuron determines the rhythm of early motor behavior in Ciona2021

    • Author(s)
      Taichi Akahoshi, Madoka K. Utsumi, Kouhei Oonuma, Makoto Murakami, Takeo Horie, Takehiro G. Kusakabe, Kotaro Oka, Kohji Hotta
    • Journal Title

      Science Advances

      Volume: 7 Issue: 50 Pages: 1-12

    • DOI

      10.1126/sciadv.abl6053

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evolution of Developmental Programs for the Midline Structures in Chordates: Insights From Gene Regulation in the Floor Plate and Hypochord Homologues of Ciona Embryos2021

    • Author(s)
      Kouhei Oonuma, Maho Yamamoto, Naho Moritsugu, Nanako Okawa, Megumi Mukai, Miku Sotani, Shuto Tsunemi, Haruka Sugimoto, Eri Nakagome, Yuichi Hasegawa, Kotaro Shimai, Takeo Horie, Takehiro G. Kusakabe
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 9 Pages: 1-12

    • DOI

      10.3389/fcell.2021.704367

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vasopressin Promoter Transgenic and Vasopressin Gene-Edited Ascidian, Ciona intestinalis Type A (Ciona robusta): Innervation, Gene Expression Profiles, and Phenotypes2021

    • Author(s)
      Kawada Tsuyoshi、Shiraishi Akira、Matsubara Shin、Hozumi Akiko、Horie Takeo、Sasakura Yasunori、Satake Honoo
    • Journal Title

      Frontiers in Endocrinology

      Volume: 12 Pages: 668564-668564

    • DOI

      10.3389/fendo.2021.668564

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] GABA-Induced GnRH Release Triggers Chordate Metamorphosis.2020

    • Author(s)
      Hozumi A, Matsunobu S, Mita K, Treen N, Sugihara T, Horie T, Sakuma T, Yamamoto T, Shiraishi A, Hamada M, Satoh N, Sakurai K, Satake H, Sasakura Y.
    • Journal Title

      Current Biology

      Volume: 30 Issue: 8 Pages: 1555-1561

    • DOI

      10.1016/j.cub.2020.02.003

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cellular identity and Ca2+ signaling activity of the non-reproductive GnRH system in the Ciona intestinalis type A (Ciona robusta) larva2020

    • Author(s)
      Okawa Nanako、Shimai Kotaro、Ohnishi Kohei、Ohkura Masamichi、Nakai Junichi、Horie Takeo、Kuhara Atsushi、Kusakabe Takehiro G.
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 18590-18590

    • DOI

      10.1038/s41598-020-75344-7

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparison of differentiation gene batteries for migratory mechanosensory neurons across bilaterians2020

    • Author(s)
      Zhao Di、Chen Siyu、Horie Takeo、Gao Yimeng、Bao Hongcun、Liu Xiao
    • Journal Title

      Evolution & Development

      Volume: 22 Issue: 6 Pages: 438-450

    • DOI

      10.1111/ede.12331

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] シングルセルトランスクリプトーム解析から切り込む視床下部相同器官の分化機構2021

    • Author(s)
      堀江健生
    • Organizer
      第41回日本比較内分泌学会大会及びシンポジウム
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] ホヤ幼生の重力を感知する神経回路の構造と機能2021

    • Author(s)
      一寸木明日香、堀江良子、笹倉靖徳、堀江健生
    • Organizer
      第44回日本分子生物学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 単一細胞トランスクリプトーム解析によるホヤ幼生の尾部に存在する双極型感覚神経細胞の分化機構の解析2021

    • Author(s)
      堀江良子、日下部岳広、笹倉靖徳、堀江健生
    • Organizer
      第44回日本分子生物学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] カタユウレイボヤにおけるNK6遺伝子の転写調節領域の解析2021

    • Author(s)
      長谷川達也,堀江良子,笹倉靖徳,堀江健生
    • Organizer
      日本動物学会第92回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 単一細胞トランスクリプトーム解析によるホヤ幼生尾部の双極型感覚神経細胞の分化機構の解析2021

    • Author(s)
      堀江良子、日下部岳広、笹倉靖徳、堀江健生
    • Organizer
      日本動物学会第92回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Regulatory cocktail for dopaminergic neurons in ascidians identified by single cell transcriptomics2020

    • Author(s)
      Takeo Horie
    • Organizer
      JSDB online trial meeting, Symposium
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 単一細胞トランスクリプトームによるホヤ胚における神経細胞分化を制御する遺伝子カクテルの同定2019

    • Author(s)
      堀江 健生
    • Organizer
      日本動物学会 第90回 大阪大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Regulatory cocktail for individual neurons identified by whole embryos single cell transcriptomics in ascidian embryos2019

    • Author(s)
      Takeo Horie
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Remarks] 堀江研究室ホームページ

    • URL

      https://cionaneuron.wixsite.com/labhomepage

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
  • [Remarks] 堀江研究室 ホームページ

    • URL

      http://accafe.jp/Horie_Takeo/index.php

    • Related Report
      2019 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2023-01-30  

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