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Phloem patterning by CLE peptides

Research Project

Project/Area Number 19H03246
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionOsaka University

Principal Investigator

Kakimoto Tatsuo  大阪大学, 大学院理学研究科, 教授 (70214260)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2021: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywordsphloem / CLE / Arabidopsis / Transcription factor / Dof / receptor / 篩部 / 篩管 / 転写因子 / 受容体 / シロイヌナズナ / ペプチド / 伴細胞 / 篩管要素 / パターン形成 / CLEペプチド / 植物 / 形態形成 / シグナル分子 / 細胞間情報伝達因子
Outline of Research at the Start

維管束は、篩部、木部、前形成層からなる。根においては、根端分裂組織における一次成長によって維管束系の基本パターンが形成される。篩部前駆細胞では複数のDof転写因子群が発現しているが、その下流因子として複数のCLEペプチド遺伝子がある。これらのCLEペプチドは篩部形成の側方阻害因子として働いているらしいことがわかってきた。そこで、維管束パターンの形成におけるCLEペプチドの役割を明らかにし、さらには、その受容体を解明することにより、維管束の組織パターンの決定の仕組みを明らかにする。

Outline of Final Research Achievements

We identified master transcription factors, phloem-Dofs, which are expressed in phloem precursors, and directs the phloem-fate commitment. Phloem-Dofs induce genes that are required for phloem formation, but also induce CLE25, 26, and 45, which inhibit phloem formation. CLE25, 26 and 45 are perceived by BAM-CIK receptor-coreceptor complexes and destabilize phloem-Dof proteins forming negative feedback. These positive and negative regulation creates the proper phloem organization.

Academic Significance and Societal Importance of the Research Achievements

維管束植物において篩部は光合成産物を運ぶ大切な器官ですが、その細胞パタンがどのように作られるのか、篩部細胞はどのようにして分化運命を獲得するのかについてはよくわかっていませんでした。本研究において、篩部形成のマスター因子が同定されたこと、拡散性のCLEペプチドによる負のフィードバックによって適切な篩部パタンが作られることが解明されたのは学術上の大きな意義があります。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (29 results)

All 2022 2021 2020 Other

All Int'l Joint Research (4 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (19 results) (of which Int'l Joint Research: 2 results,  Invited: 9 results) Remarks (1 results)

  • [Int'l Joint Research] Tsinghua University(中国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Max Planck Institut(ドイツ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Max plank fur plant breeding research(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] 清華大学(中国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Pericycle cell division competence underlies various developmental programs2022

    • Author(s)
      Zhang Y, Umeda M, Kakimoto T.
    • Journal Title

      Plant Biotechnology

      Volume: 39 Issue: 1 Pages: 29-36

    • DOI

      10.5511/plantbiotechnology.21.1202a

    • NAID

      130008161264

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2022-03-25
    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Dof-CLE circuit controls phloem organization.2022

    • Author(s)
      Qian P, Song W, Zaizen-Iida M, Kume S, Wang G, Zhang Y, Kinoshita-Tsujimura K, Chai J, Kakimoto T
    • Journal Title

      Nature Plants

      Volume: 8 Issue: 7 Pages: 817-827

    • DOI

      10.1038/s41477-022-01176-0

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] ROP Interactive Partners are Involved in Control of Cell Division Pattern in Arabidopsis Leaves.2022

    • Author(s)
      Hasi Q, Kakimoto T
    • Journal Title

      Plant Cell Physiology

      Volume: 63 Issue: 8 Pages: 1130-1139

    • DOI

      10.1093/pcp/pcac089

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two types of bHLH transcription factor determine the competence of the pericycle for lateral root initiation.2021

    • Author(s)
      Zhang Y, Mitsuda N, Yoshizumi T, Horii Y, Oshima Y, Ohme-Takagi, M, Matsui M, Kakimoto, T.
    • Journal Title

      Nature Plants

      Volume: 7 Issue: 5 Pages: 633-643

    • DOI

      10.1038/s41477-021-00919-9

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Early Endosomal Trafficking Component BEN2/VPS45 Plays a Crucial Role in Internal Tissues in Regulating Root Growth and Meristem Size in Arabidopsis2020

    • Author(s)
      Matsuura Yuki、Fukasawa Narumi、Ogita Kosuke、Sasabe Michiko、Kakimoto Tatsuo、Tanaka Hirokazu
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 1027-1027

    • DOI

      10.3389/fpls.2020.01027

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 側根原基形成における局所的なオーキシン生合成の役割2022

    • Author(s)
      金田紗苗,柿本辰男
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
  • [Presentation] A Dof-CLE circuit controls phloem organization2022

    • Author(s)
      Pingping Qian, Tatsuo Kakimoto
    • Organizer
      第86回 日本植物学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 内鞘細胞の側根形成能を支配するPFA/PFB転写因子の下流遺伝子の解析2022

    • Author(s)
      金田紗苗、柿本辰男
    • Organizer
      第86回 日本植物学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 内鞘細胞の側根形成能を支配するPFA/PFB転写因子の下流遺伝子の解析2022

    • Author(s)
      山本凜、柿本辰男
    • Organizer
      近畿植物学会 第 11 回 講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 側根創始細胞の核の移動におけるオーキシンの役割2022

    • Author(s)
      金田紗苗、柿本辰男
    • Organizer
      近畿植物学会 第 11 回 講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Molecular basis of the competence for lateral root formation2022

    • Author(s)
      Tatsuo Kakimoto
    • Organizer
      29th FAOBMB & the 2022 CSBMB Conference
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] PFAs and PFBs, two types of bHLH proteins, determine the competence of pericycle for lateral root initiation2021

    • Author(s)
      Ye Zhang , Nobutaka Mitsuda , Takeshi Yoshizumi , Yoko Horii , Yoshimi Oshima , Masaru Ohme-Takagi , Minami Matsui , Tatsuo Kakimoto
    • Organizer
      Secrets of stem cells underlying longevity and persistent growth in plants
    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 転写因子-ペプチド-受容体-転写因子フィードバックループによる篩部パターン形 成の仕組み2021

    • Author(s)
      柿本辰男
    • Organizer
      近畿植物学会 第 10 回 講演会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 側根原基形成における局所的なオーキシン生合成の役割2021

    • Author(s)
      金田紗苗 柿本辰男
    • Organizer
      近畿植物学会 第 10 回 講演会
    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
  • [Presentation] シロイヌナズナの側根形成初期に発現するGATA23遺伝子の破壊株の解析2021

    • Author(s)
      山本凜、柿本辰男
    • Organizer
      近畿植物学会 第 10 回 講演会
    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
  • [Presentation] ROP interactive partners (RIPs) regulate microtubule dynamics and orientation of cell division in the leaves2021

    • Author(s)
      ハスチムグ、柿本辰男
    • Organizer
      第62回日本植物生理学会
    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Presentation] 転写因子-ペプチド-受容体-転写因子フィードバックループによる篩部パターン形成の仕組み2021

    • Author(s)
      柿本辰男
    • Organizer
      近畿植物学会 第 10 回 講演会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] PFAs and PFBs, two types of bHLH proteins, determine the competence of pericycle for lateral root initiation2021

    • Author(s)
      Ye Zhang , Nobutaka Mitsuda , Takeshi Yoshizumi , Yoko Horii , Yoshimi Oshima , Masaru Ohme-Takagi , Minami Matsui , Tatsuo Kakimoto
    • Organizer
      国際シンポジウム Secrets of stem cells underlying longevity and persistent growth in plants
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Regulation of vascular development by CLE peptides2020

    • Author(s)
      柿本辰男
    • Organizer
      日本植物学会84回大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Transcription factors that govern the stem-cell-like features of pericycle2020

    • Author(s)
      柿本辰男
    • Organizer
      第61回 日本植物生理学会 サテライトシンポジウム
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナの葉形態形成におけるR I P タンパク質の機能解析2020

    • Author(s)
      哈斯其木格,柿本辰男
    • Organizer
      第61回日本植物生理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Identification of key transcription factors that determine pericycle stem cell identity in Arabidopsis.2020

    • Author(s)
      Ye Zhang, Nobutaka Mitsuda, Takeshi Yoshizumi, Youichi Kondou, Masaru Takagi, Minami Matsui, Tatsuo Kakimoto
    • Organizer
      第61回日本植物生理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Regulation of vascular development by CLE peptides2020

    • Author(s)
      柿本辰男
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Transcription factors that govern the stem-cell-like features of pericycle2020

    • Author(s)
      Tatsuo Kakimoto
    • Organizer
      第61回 日本植物生理学会 サテライトシンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Remarks] Kakimoto Lab

    • URL

      https://kakimoto0.wixsite.com/kakimoto-lab

    • Related Report
      2021 Annual Research Report

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Published: 2019-04-18   Modified: 2024-12-25  

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