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Chemical biology study on auxin inactivation pathway

Research Project

Project/Area Number 19H03253
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionOkayama University of Science

Principal Investigator

Ken-ichiro Hayashi  岡山理科大学, 生命科学部, 教授 (30289136)

Co-Investigator(Kenkyū-buntansha) 笠原 博幸  東京農工大学, (連合)農学研究科(研究院), 教授 (00342767)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2022: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2021: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2020: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2019: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Keywordsオーキシン / 植物ホルモン / 代謝経路 / 不活性化 / ケミカルバイオロジー
Outline of Research at the Start

植物の複雑な形態形成や多様な環境応答にはオーキシンの組織・細胞濃度の動的制御が重要である。オーキシンの組織・細胞濃度は、(1)IAA(内生オーキシン)の生合成,(2) IAAの極性輸送(3)IAAの不活性化の3つのステップにより、協調して精緻に調節されている。オーキシン生合成経路と極性輸送は明らかにされた一方で、IAAの不活性化については、その生理的意義をふくめて、オーキシン不活性化経路の全体像はいまだ決定されていない。本研究ではケミカルバイオロジーと分子遺伝学的手法を統合・活用して,オーキシン不活性化経路の決定とその生理的意義の解明に挑み、食糧生産の改善に期待できる植物の生長制御のしくみを解き明かす。

Outline of Final Research Achievements

Indole-3-acetic acid (IAA), a natural auxin is a potent plant hormone that regulates diverse aspects of plant development and growth. The hormonal activity of IAA is modulated by three pathways: biosynthesis, transport, and inactivation of IAA. To date, the IAA inactivation pathway has not been fully elucidated. In this work, we investigated the IAA inactivating enzymes by using molecular genetic and chemical biology approach to elucidate the metabolic inactivation pathway of IAA. Our study demonstrate that IAA is inactivated via a new auxin metabolic pathway designated as GH3-ILR1-DAO pathway in plants.

Academic Significance and Societal Importance of the Research Achievements

植物の成長の鍵となるホルモン・オーキシンが,どのように不活性化・一時貯蔵・再生・分解されているのか?という植物学や農学において重要な謎が解明されたことで,オーキシンによる植物の複雑な成長調節メカニズムの解明がさらに進むと考えらる。オーキシンは,成長促進剤,発根促進剤さらに除草剤として農業分野で幅広く利用されている。今回の研究成果から,より活性の高い発根促進剤などの開発につながると期待できる。さらに,オーキシンの不活性化・分解をコントロールすることで,農作物,綿花などの衣料原料,樹木バイオマスなどを増産する新たな研究の道が拓かれると期待される。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (38 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (7 results) Journal Article (13 results) (of which Int'l Joint Research: 10 results,  Peer Reviewed: 13 results,  Open Access: 8 results) Presentation (16 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Remarks (2 results)

  • [Int'l Joint Research] Washington University/University of California San Diego(米国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] University of Warwick(英国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] University of California San Diego(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] University of Warwick/University of Leeds(英国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] University of California San Diego(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Warwick/University of Leeds(英国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] カルフォルニア大学サンディエゴ校(米国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Auxin Regulates Apical Stem Cell Regeneration and Tip Growth in the Marine Red Alga Neopyropia yezoensis2022

    • Author(s)
      Taya K, Takeuchi S, Takahashi M, Hayashi KI, Mikami K.
    • Journal Title

      Cells

      Volume: 11 Issue: 17 Pages: 2652-2652

    • DOI

      10.3390/cells11172652

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fluorescent Auxin Analogs Report Two Auxin Binding Sites with Different Subcellular Distribution and Affinities: A Cue for Non-Transcriptional Auxin Signaling2022

    • Author(s)
      Huang X, Maisch J, Hayashi KI, Nick P.
    • Journal Title

      Int J Mol Sci.

      Volume: 23 Issue: 15 Pages: 8593-8593

    • DOI

      10.3390/ijms23158593

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chemical inhibition of the auxin inactivation pathway uncovers the roles of metabolic turnover in auxin homeostasis2022

    • Author(s)
      Fukui K, Arai K, Tanaka Y, Aoi Y, Kukshal V, Jez JM, Kubes MF, Napier R, Zhao Y, Kasahara H, Hayashi KI.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 119 Issue: 32

    • DOI

      10.1073/pnas.2206869119

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The main oxidative inactivation pathway of the plant hormone auxin2021

    • Author(s)
      Hayashi KI, Arai K, Aoi Y, Tanaka Y, Hira H, Guo R, Hu Y, Ge C, Zhao Y, Kasahara H, Fukui K.
    • Journal Title

      Nat Commun

      Volume: 12(1) Issue: 1 Pages: 6752-6752

    • DOI

      10.1038/s41467-021-27020-1

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Local regulation of auxin transport in root-apex transition zone mediates aluminium-induced Arabidopsis root-growth inhibition2021

    • Author(s)
      Li C, Liu G, Geng X, He C, Quan T, Hayashi KI, De Smet I, Robert HS, Ding Z, Yang ZB.
    • Journal Title

      Plant J.

      Volume: 108(1) Issue: 1 Pages: 55-56

    • DOI

      10.1111/tpj.15424

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Chemical Biology in Auxin Research.2021

    • Author(s)
      Hayashi KI.
    • Journal Title

      Cold Spring Harb Perspect Biol

      Volume: 13(5) Issue: 5 Pages: a040105-a040105

    • DOI

      10.1101/cshperspect.a040105

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] UDP-glucosyltransferase UGT84B1 regulates the levels of indole-3-acetic acid and phenylacetic acid in Arabidopsis2020

    • Author(s)
      Aoi Yuki、Hira Hayao、Hayakawa Yuya、Liu Hongquan、Fukui Kosuke、Dai Xinhua、Tanaka Keita、Hayashi Ken-ichiro、Zhao Yunde、Kasahara Hiroyuki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 532 Issue: 2 Pages: 244-250

    • DOI

      10.1016/j.bbrc.2020.08.026

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An evolutionarily primitive and distinct auxin metabolism in the lycophyte Selaginella moellendorffii2020

    • Author(s)
      Kaneko Shutaro、Cook Sam David、Aoi Yuki、Watanabe Akie、Hayashi Ken-Ichiro、Kasahara Hiroyuki
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 10 Pages: 1724-1732

    • DOI

      10.1093/pcp/pcaa098

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Role of Arabidopsis INDOLE-3-ACETIC ACID CARBOXYL METHYLTRANSFERASE 1 in auxin metabolism2020

    • Author(s)
      Takubo Eiko、Kobayashi Makoto、Hirai Shoko、Aoi Yuki、Ge Chennan、Dai Xinhua、Fukui Kosuke、Hayashi Ken-ichiro、Zhao Yunde、Kasahara Hiroyuki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 527 Issue: 4 Pages: 1033-1038

    • DOI

      10.1016/j.bbrc.2020.05.031

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Endogenous auxin determines the pattern of adventitious shoot formation on internodal segments of ipecac2020

    • Author(s)
      Koike I, Watanabe S, Okazaki K, Hayashi KI, Kasahara H, Shimomura K, Umehara M.
    • Journal Title

      Planta . 2020 Mar 5;251(3):73.

      Volume: 251(3) Issue: 3 Pages: 73-73

    • DOI

      10.1007/s00425-020-03367-5

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arogenate dehydratases can modulate the levels of phenylacetic acid in Arabidopsis2020

    • Author(s)
      Aoi Yuki、Oikawa Akira、Sasaki Ryosuke、Huang Jirong、Hayashi Ken-ichiro、Kasahara Hiroyuki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 524 Issue: 1 Pages: 83-88

    • DOI

      10.1016/j.bbrc.2020.01.041

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] GH3 auxin-amido synthetases alter the ratio of indole-3-acetic acid and phenylacetic acid in Arabidopsis2019

    • Author(s)
      Aoi Yuki、Tanaka Keita、Cook Sam David、Hayashi Ken-Ichiro、Kasahara Hiroyuki
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 3 Pages: 596-605

    • DOI

      10.1093/pcp/pcz223

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization2019

    • Author(s)
      Oochi Akihiro、Hajny Jakub、Fukui Kosuke、Nakao Yukio、Gallei Michelle、Quareshy Mussa、Takahashi Koji、Kinoshita Toshinori、Harborough Sigurd Ramans、Kepinski Stefan、Kasahara Hiroyuki、Napier Richard、Friml Ji??、Hayashi Ken-ichiro
    • Journal Title

      Plant Physiology

      Volume: 180 Issue: 2 Pages: 1152-1165

    • DOI

      10.1104/pp.19.00201

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Chemical Biology of Indole Derivatives from Plant Hormone to Medicine2022

    • Author(s)
      Ken-ichiro Hayashi
    • Organizer
      The 37th Symposium on Natural Products
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 胞子植物におけるオーキシン不活化経路の解析2022

    • Author(s)
      菅沼 有紀、丸山 海成、嶋村 正樹、林 謙一郎、笠原 博幸
    • Organizer
      植物化学調節学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 標的細胞・オルガネラへの代謝活性化を利用したオーキシンデリバリー制御系の構築2022

    • Author(s)
      田中佑佳,江田 智彬,前田 雅志,古川 毅,福井康祐,三井亮司,林謙一郎
    • Organizer
      日本農芸化学会中四国支部第61回講演会(例会)
    • Related Report
      2021 Annual Research Report
  • [Presentation] 単子葉・双子葉植物におけるオーキシン代謝産物の分析2022

    • Author(s)
      田中佑佳,新井一司, 福井康祐,青井勇輝,比良 隼, Yunde Zhao,笠原博幸,林謙一郎
    • Organizer
      日本農薬学会第47回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] オーキシンの不活性化経路の再構築に関する研究2022

    • Author(s)
      田中佑佳,新井一司, 福井康祐,Ruipan Guo, Yun Hu, Chennan Ge,青井勇輝,比良 隼, Yunde Zhao,笠原博幸,林謙一郎
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] オーキシンの酸化的代謝経路に関する研究(II)2021

    • Author(s)
      田中 佑佳, 福井 康祐, 新井 一司, 村上 寿基, Ruipan Guo, Yun Hu, Chennan Ge,青井 勇輝, 比良 隼, Yunde Zhao, 笠原 博幸, 林 謙一郎
    • Organizer
      植物化学調節学会 第56回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] シロイヌナズナ根の重力変化に応答したオーキシン不等分形成におけるインドール酪酸輸送体 NPF7.3/NRT1.5 の寄与2021

    • Author(s)
      渡邊 俊介, 高橋 直紀, 菅野 裕理 , 鈴木 洋弥, 青井 勇輝, 武田 紀子, 豊岡 公徳, 笠原 博幸, 林 謙 一郎, 梅田 正明, 瀬尾 光範
    • Organizer
      植物生理学会 2021年大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 2020/09 活性分子の可視化プローブのデザイン プローブでどのような情報を可視化するのか。2020

    • Author(s)
      林謙一郎
    • Organizer
      情報分子可視化技術勉強会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナのオーキシン濃度調節におけるUDP-グルコシル基転移酵素UGT84B1の役割2020

    • Author(s)
      比良隼 , 青井勇輝 , 早川雄也 , Hongquan Liu , 福井康佑 , Xinhua Dai , 田中慧太 , 林謙一郎 , Yunde Zhao ,笠原博幸
    • Organizer
      植物化学調節学会 第55回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] IAA-アミノ酸複合体の植物体内における生理機能の解析2020

    • Author(s)
      新井一司, 田中佑佳, 村上寿基, 川渕 拓, 青井勇輝, 笠原博幸, 福井康祐, 林謙一郎
    • Organizer
      植物化学調節学会 第55回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 細胞内IAA濃度の恒常性はGH3アミノ酸複合体合成酵素によって調節される2020

    • Author(s)
      新井一司, 青井勇輝, 笠原博幸, 福井康祐, 林謙一郎
    • Organizer
      農芸化学会 中四国支部大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物ホルモン・オーキシンのケミカルバイオロジー2019

    • Author(s)
      林謙一郎
    • Organizer
      日本農芸化学会中四国支部第54回講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 植物ホルモン・オーキシンの再活性化酵素に関する研究2019

    • Author(s)
      村上寿基,新井一司,川渕 拓,笠原博幸,福井康祐,林謙一郎
    • Organizer
      日本農芸化学会中四国支部第54回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] オーキシン代謝酵素GH3に対する阻害剤の速度論的解析2019

    • Author(s)
      新井一司,赤嶺孝太, 福井康祐,笠原博幸,林謙一郎
    • Organizer
      日本農芸化学会中四国支部第54回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] オーキシンーアミノ酸複合体合成酵素GH3の 阻害剤を用いたIAA代謝経路の解析2019

    • Author(s)
      福井康祐、新井一司、青井勇輝、竹林裕美子、笠原博幸、林謙一郎
    • Organizer
      植物化学調節学会第54回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] オーキシン-アミノ酸複合体合成酵素GH3の阻害剤の速度論的解析2019

    • Author(s)
      新井一司、青井勇輝、竹林裕美子、笠原博幸、福井康祐、林謙一郎
    • Organizer
      植物化学調節学会第54回大会
    • Related Report
      2019 Annual Research Report
  • [Remarks] 植物ホルモン「オーキシン」が植物の中で生合成・不活性化 される速度を決定

    • URL

      https://www.life.ous.ac.jp/wp-content/uploads/2022/08/KAKEIMIDE.pdf

    • Related Report
      2022 Annual Research Report
  • [Remarks] 植物ホルモン「オーキシン」をリサイクル・分解する 経路を解明

    • URL

      https://www.ous.ac.jp/common/files//202111261122590714616.pdf

    • Related Report
      2021 Annual Research Report

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Published: 2019-04-18   Modified: 2024-01-30  

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