Imaging analyses of chromatin dynamics in plant regeneration

• Project/Area Number: 19H03259
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 44040:Morphology and anatomical structure-related
• Research Institution: The University of Tokyo
• Principal Investigator: Matsunaga Sachihiro 東京大学, 大学院新領域創成科学研究科, 教授 (40323448)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2021: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2019: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: イメージング / ヒストン修飾 / 再生 / エピジェネティクス / 転写活性化 / プライミング / クロマチン / エピジェネティックス / カルス

Outline of Research at the Start

植物の強力な再分化能力を制御する遺伝子プライミング現象を発見し、その制御因子であるヒストン脱メチル化酵素LDL3を同定した（Nature Communications 2019)。シュート再生前のカルスの段階で、プライミング遺伝子群が転写スタンバイになりシュート形成時に一気に遺伝子発現がONになる。TOMEIを基盤としたディープイメージングや遺伝子座特異的クロマチン可視化技術を用いて、多細胞塊のカルスを研究材料にして解析する。この解析により従来の研究枠組みを突破し、組織内の細胞運命転換という形態形成の根本メカニズムを裏打ちするクロマチン構造変換の実態を明らかにする。

Outline of Final Research Achievements

The molecular mechanism of epigenetic priming, a phenomenon in which plants put gene expression on standby when they regenerate, has been identified. We found that this epigenetic priming is regulated by the histone demethylase LYSINE-SPECIFIC DEMETHYLASE 1-LIKE 3 (LDL3) (Nature Commun., 2019). We also developed a method for live imaging analysis of the phosphorylation at Ser2 for activation of RNA polymerase II (RNAPII) (Commun. Biol., 2021). As a result, we were able to verify that RNAPII is activated in the shoot apical meristem of the callus.

Academic Significance and Societal Importance of the Research Achievements

植物は切断しても器官を新たに再生できる非常に強力な再生能力を持つ。この再生能力のメカニズムは、将来、器官再生するために、あらかじめ遺伝子の発現のスイッチをONにする手前の待機状態にしていることがわかった。この待機状態の解明により、植物の強靭な再生力のメカニズムの一端が明らかになり、植物の生きざまの理解を促進した学術的意義がある。また、遺伝子の転写がONになった時を知るライブイメージング解析技術や、器官や組織のディープイメージングを可能にする透明化手法を開発することで、植物科学の研究推進することに貢献する技術開発を行った。同時に、これらの技術は、農作物の品種改良などの社会的貢献も期待される。

Research Products

• [Int'l Joint Research] Institute of Experimental Botany/Masaryk University(チェコ)
• [Int'l Joint Research] Zurich University(スイス)
• [Int'l Joint Research] チューリッヒ大学(スイス)
• [Int'l Joint Research] カリフォルニア工科大学(米国)
• [Journal Article] Improved clearing method contributes to deep imaging of plant organs (2022)
  • Author(s): Sakamoto Yuki、Ishimoto Anna、Sakai Yuuki、Sato Moeko、Nishihama Ryuichi、Abe Konami、Sano Yoshitake、Furuichi Teiichi、Tsuji Hiroyuki、Kohchi Takayuki、Matsunaga Sachihiro
  • Journal Title: Communications Biology Volume: 5 Issue: 1 Pages: 12-12
  • DOI: 10.1038/s42003-021-02955-9
  • Peer Reviewed / Open Access
• [Journal Article] Arabidopsis TBP-ASSOCIATED FACTOR 12 ortholog NOBIRO6 controls root elongation with unfolded protein response cofactor activity (2022)
  • Author(s): Kim June-Sik、Sakamoto Yuki、Takahashi Fuminori、Shibata Michitaro、Urano Kaoru、Matsunaga Sachihiro、Yamaguchi-Shinozaki Kazuko、Shinozaki Kazuo
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 119 Issue: 6
  • DOI: 10.1073/pnas.2120219119
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sustained defense response via volatile signaling and its epigenetic transcriptional regulation (2022)
  • Author(s): Onosato, Haruki; Fujimoto, Genya; Higami, Tomota; Sakamoto, Takuya; Yamada, Ayaka; Suzuki, Takamasa; Ozawa, Rika; Matsunaga, Sachihiro; Seki, Motoaki; Ueda, Minoru; Sako, Kaori; Galis, Ivan; Arimura, Gen-ichiro
  • Journal Title: Plant Physiology Volume: － Issue: 2 Pages: 922-933
  • DOI: 10.1093/plphys/kiac077
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Next Generation Sequence-based Technologies for Analyzing DNA Strand Breaks (2021)
  • Author(s): Y. Fujiwara, S. Matsunaga, and T. Sakamoto
  • Journal Title: CYTOLOGIA Volume: 86 Issue: 1 Pages: 3-9
  • DOI: 10.1508/cytologia.86.3
  • NAID: 130008002777
  • ISSN: 0011-4545, 1348-7019
  • Year and Date: 2021-03-25
  • Peer Reviewed / Open Access
• [Journal Article] Thiazoline-related innate fear stimuli orchestrate hypothermia and anti-hypoxia via sensory TRPA1 activation (2021)
  • Author(s): Matsuo Tomohiko、Isosaka Tomoko、Hayashi Yuichiro、Tang Lijun、Doi Akihiro、Yasuda Aiko、Hayashi Mikio、Lee Chia-Ying、Cao Liqin、Kutsuna Natsumaro、Matsunaga Sachihiro、Matsuda Takeshi、Yao Ikuko、Setou Mitsuyoshi、Kanagawa Dai、Higasa Koichiro、Ikawa Masahito、Liu Qinghua、Kobayakawa Reiko、Kobayakawa Ko
  • Journal Title: Nature Communications Volume: 12 Issue: 1 Pages: 2074-2074
  • DOI: 10.1038/s41467-021-22205-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A live imaging system to analyze spatiotemporal dynamics of RNA polymerase II modification in Arabidopsis thaliana (2021)
  • Author(s): M. Shibuta, T. Sakamoto, T. Yamaoka, M. Yoshikawa, S. Kasamatsu, N. Yagi, S. Fujimoto, T. Suzuki, S. Uchino, Y. Sato. H. Kimura, and S. Matsunaga
  • Journal Title: Communications Biology Volume: 4 Issue: 1 Pages: 580-580
  • DOI: 10.1038/s42003-021-02106-0
  • Peer Reviewed / Open Access
• [Journal Article] An anchoring complex recruits katanin for microtubule severing at the plant cortical nucleation sites (2021)
  • Author(s): Noriyoshi Yagi , Takehide Kato , Sachihiro Matsunaga , David W. Ehrhardt , Masayoshi Nakamura , Takashi Hashimoto
  • Journal Title: Nature Communications Volume: 12 Issue: 1 Pages: 3684-3684
  • DOI: 10.1038/s41467-021-24067-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Whole-tissue three-dimensional imaging of rice at single-cell resolution (2021)
  • Author(s): Moeko Sato, Hiroko Akashi, Yuki Sakamoto, Sachihiro Matsunaga, Hiroyuki Tsuji
  • Journal Title: International Journal of Molecular Sciences Volume: 23 Issue: 1 Pages: 40-40
  • DOI: 10.3390/ijms23010040
  • Peer Reviewed / Open Access
• [Journal Article] SQAP, an acyl sulfoquinovosyl derivative, suppresses expression of histone deacetylase and induces cell death of cancer cells under hypoxic conditions (2021)
  • Author(s): Kawakubo Hirofumi、Kamisuki Shinji、Suzuki Kei、Izaguirre-Carbonell Jesus、Saito Shiki、Murata Hiroshi、Tanabe Atsushi、Hongo Ayumi、Murakami Hironobu、Matsunaga Sachihiro、Sakaguchi Kengo、Sahara Hiroeki、Sugawara Fumio、Kuramochi Kouji
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 85 Issue: 1 Pages: 85-91
  • DOI: 10.1093/bbb/zbaa015
  • Peer Reviewed
• [Journal Article] Common architectures in cyanobacteria Prochlorococcus cells visualized by X-ray diffraction imaging using X-ray free electron laser (2021)
  • Author(s): Kobayashi Amane、Takayama Yuki、Hirakawa Takeshi、Okajima Koji、Oide Mao、Oroguchi Tomotaka、Inui Yayoi、Yamamoto Masaki、Matsunaga Sachihiro、Nakasako Masayoshi
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 3877-3877
  • DOI: 10.1038/s41598-021-83401-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A live imaging system to analyze spatiotemporal dynamics of RNA polymerase II modification in Arabidopsis thaliana. (2021)
  • Author(s): Shibuta, M. K., Sakamoto, T., Yamaoka, T., Yoshikawa, M., Kasamatsu, S., Yagi, N., Fujimoto, S., Suzuki, T., Uchino, S., Sato, Y., Kimura, H. and Matsunaga, S.
  • Journal Title: Commun. Biol Volume: -
  • Peer Reviewed / Open Access
• [Journal Article] Roles of BRAHMA and Its Interacting Partners in Plant Chromatin Remodeling (2020)
  • Author(s): S. Nishioka, T. Sakamoto, and S. Matsunaga
  • Journal Title: CYTOLOGIA Volume: 85 Issue: 4 Pages: 263-267
  • DOI: 10.1508/cytologia.85.263
  • NAID: 130007957724
  • ISSN: 0011-4545, 1348-7019
  • Year and Date: 2020-12-25
  • Peer Reviewed / Open Access
• [Journal Article] Mitotic Karyotype of the Primitive Red Alga <i>Cyanidioschyzon merolae</i> 10D (2020)
  • Author(s): T. Kuroiwa, F. Yagisawa, T. Fujiwara, Y. Inui, T.M. Matsunaga, S. Katoi, S. Matsunaga, N. Nagata, Y. Imoto, H. Kuroiwa
  • Journal Title: CYTOLOGIA Volume: 85 Issue: 2 Pages: 107-113
  • DOI: 10.1508/cytologia.85.107
  • NAID: 130007864023
  • ISSN: 0011-4545, 1348-7019
  • Year and Date: 2020-06-25
  • Peer Reviewed
• [Journal Article] Deep Imaging Analysis in VISUAL Reveals the Role of YABBY Genes in Vascular Stem Cell Fate Determination (2020)
  • Author(s): Nurani Alif Meem、Ozawa Yasuko、Furuya Tomoyuki、Sakamoto Yuki、Ebine Kazuo、Matsunaga Sachihiro、Ueda Takashi、Fukuda Hiroo、Kondo Yuki
  • Journal Title: Plant and Cell Physiology Volume: 61 Issue: 2 Pages: 255-264
  • DOI: 10.1093/pcp/pcaa002
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The formation of perinucleolar bodies is important for normal leaf development and requires the zinc‐finger DNA‐binding motif in Arabidopsis ASYMMETRIC LEAVES2 (2020)
  • Author(s): Luo Lilan、Ando Sayuri、Sakamoto Yuki、Suzuki Takanori、Takahashi Hiro、Ishibashi Nanako、Kojima Shoko、Kurihara Daisuke、Higashiyama Tetsuya、Yamamoto Kotaro T.、Matsunaga Sachihiro、Machida Chiyoko、Sasabe Michiko、Machida Yasunori
  • Journal Title: The Plant Journal Volume: 101 Issue: 5 Pages: 1118-1134
  • DOI: 10.1111/tpj.14579
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Two combinatorial patterns of telomere histone marks in plants with canonical and non‐canonical telomere repeats (2020)
  • Author(s): Adamusov? Kate?ina、Khosravi Solmaz、Fujimoto Satoru、Houben Andreas、Matsunaga Sachihiro、Fajkus Ji??、Fojtov? Miloslava
  • Journal Title: The Plant Journal Volume: 102 Issue: 4 Pages: 678-687
  • DOI: 10.1111/tpj.14653
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Subnuclear gene positioning through lamina association affects copper tolerance (2020)
  • Author(s): Sakamoto Yuki、Sato Mayuko、Sato Yoshikatsu、Harada Akihito、Suzuki Takamasa、Goto Chieko、Tamura Kentaro、Toyooka Kiminori、Kimura Hiroshi、Ohkawa Yasuyuki、Hara-Nishimura Ikuko、Takagi Shingo、Matsunaga Sachihiro
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 5914-5914
  • DOI: 10.1038/s41467-020-19621-z
  • Peer Reviewed / Open Access
• [Journal Article] Visualization of extracellular vesicles in the regenerating caudal fin blastema of zebrafish using in vivo electroporation. (2020)
  • Author(s): Ohgo, S., Sakamoto, T., Nakajima,W., Matsunaga, S. and Wada, N.
  • Journal Title: Biochemical and biophysical research communications Volume: 533 Issue: 4 Pages: 1371-1377
  • DOI: 10.1016/j.bbrc.2020.10.024
  • Peer Reviewed
• [Journal Article] エピジェネティック・プライミングによる植物再生制御 (2020)
  • Author(s): 三浦理奈、松永幸大
  • Journal Title: ケミカルエンジニヤリング Volume: 65 Pages: 405-409
• [Journal Article] Plant regeneration by epigenetic priming (2020)
  • Author(s): 松永幸大
  • Journal Title: PLANT MORPHOLOGY Volume: 32 Issue: 1 Pages: 53-57
  • DOI: 10.5685/plmorphol.32.53
  • NAID: 130008006484
  • ISSN: 0918-9726, 1884-4154
  • Peer Reviewed
• [Journal Article] Lysine-Specific Demethylase Epigenetically Regulates Human and Plant Phenomena (2019)
  • Author(s): Toyoda, Y. and Matsunaga, S.
  • Journal Title: CYTOLOGIA Volume: 84 Issue: 4 Pages: 295-298
  • DOI: 10.1508/cytologia.84.295
  • NAID: 130007769556
  • ISSN: 0011-4545, 1348-7019
  • Year and Date: 2019-12-25
  • Peer Reviewed
• [Journal Article] 2A Peptides Contribute to the Co-Expression of Proteins for Imaging and Genome Editing (2019)
  • Author(s): Shibuta, M. K., Matsuoka, M. and Matsunaga S.
  • Journal Title: CYTOLOGIA Volume: 84 Issue: 2 Pages: 107-111
  • DOI: 10.1508/cytologia.84.107
  • NAID: 130007666602
  • ISSN: 0011-4545, 1348-7019
  • Year and Date: 2019-06-25
  • Peer Reviewed
• [Journal Article] Seasonal and Diurnal Regulation of Flowering <i>via</i> an Epigenetic Mechanism in <i>Arabidopsis thaliana</i> (2019)
  • Author(s): Shibuta, K. M. and Matsunaga, S.
  • Journal Title: CYTOLOGIA Volume: 84 Issue: 1 Pages: 3-8
  • DOI: 10.1508/cytologia.84.3
  • NAID: 130007628780
  • ISSN: 0011-4545, 1348-7019
  • Year and Date: 2019-03-25
  • Peer Reviewed
• [Journal Article] The Progression of Xylem Vessel Cell Differentiation is Dependent on the Activity Level of VND7 in Arabidopsis thaliana (2019)
  • Author(s): Hirai Risaku、Higaki Takumi、Takenaka Yuto、Sakamoto Yuki、Hasegawa Junko、Matsunaga Sachihiro、Demura Taku、Ohtani Misato
  • Journal Title: Plants Volume: 9 Issue: 1 Pages: 39-39
  • DOI: 10.3390/plants9010039
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Homologous pairing activities of Arabidopsis thaliana RAD51 and DMC1. (2019)
  • Author(s): Kobayashi, W., Liu, E., Ishii, H., Matsunaga, S., Schlogelhofer, P., and Kurumizaka, H.
  • Journal Title: J. Biochem. Volume: 165 Issue: 3 Pages: 289-295
  • DOI: 10.1093/jb/mvy105
  • NAID: 40021845884
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Acetic Acid Treatment Enhances Drought Avoidance in Cassava (Manihot esculenta Crantz) (2019)
  • Author(s): Utsumi Yoshinori、Utsumi Chikako、Tanaka Maho、Ha Chien Van、Takahashi Satoshi、Matsui Akihiro、Matsunaga Tomoko M.、Matsunaga Sachihiro、Kanno Yuri、Seo Mitsunori、Okamoto Yoshie、Moriya Erika、Seki Motoaki
  • Journal Title: Frontiers in Plant Science Volume: 10 Pages: 521-521
  • DOI: 10.3389/fpls.2019.00521
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Plant condensin II is required for the correct spatial relationship between centromeres and rDNA arrays (2019)
  • Author(s): Sakamoto Takuya、Sugiyama Tomoya、Yamashita Tomoe、Matsunaga Sachihiro
  • Journal Title: Nucleus Volume: 10 Issue: 1 Pages: 116-125
  • DOI: 10.1080/19491034.2019.1616507
  • Peer Reviewed / Open Access
• [Journal Article] The 26S Proteasome Is Required for the Maintenance of Root Apical Meristem by Modulating Auxin and Cytokinin Responses Under High-Boron Stress (2019)
  • Author(s): Sakamoto Takuya、Sotta Naoyuki、Suzuki Takamasa、Fujiwara Toru、Matsunaga Sachihiro
  • Journal Title: Frontiers in Plant Science Volume: 10 Pages: 590-590
  • DOI: 10.3389/fpls.2019.00590
  • Peer Reviewed / Open Access
• [Journal Article] Abnormal leaf development of rpt5a mutant under zinc deficiency reveals important role of DNA damage alleviation for normal leaf development (2019)
  • Author(s): Sotta Naoyuki、Sakamoto Takuya、Matsunaga Sachihiro、Fujiwara Toru
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 9369-9369
  • DOI: 10.1038/s41598-019-44789-w
  • Peer Reviewed / Open Access
• [Journal Article] The SMC5/6 Complex Subunit NSE4A Is Involved in DNA Damage Repair and Seed Development (2019)
  • Author(s): D?az Mariana、Pe?inkov? Petra、Nowicka Anna、Baroux C?lia、Sakamoto Takuya、Gandha Priscilla Yuliani、Je??bkov? Hana、Matsunaga Sachihiro、Grossniklaus Ueli、Pecinka Ales
  • Journal Title: The Plant Cell Volume: 31 Issue: 7 Pages: 1579-1597
  • DOI: 10.1105/tpc.18.00043
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of DNA Repair Foci in Root Cells of Arabidopsis in Response to DNA Damage (2019)
  • Author(s): Hirakawa Takeshi、Matsunaga Sachihiro
  • Journal Title: Frontiers in Plant Science Volume: 10 Pages: 990-990
  • DOI: 10.3389/fpls.2019.00990
  • Peer Reviewed / Open Access
• [Journal Article] Intracellular localization of histone deacetylase HDA6 in plants. (2019)
  • Author(s): Kurita, K., Sakamoto, Y., Naruse, S., Matsunaga, T. M., Arata, H., Higashiyama, T., Habu, Y., Utsumi, Y., Utsumi, C., Tanaka, M., Takahashi, S., Kim, J. M., Seki, M., Sakamoto, T., and Matsunaga, S.
  • Journal Title: J PLANT RES Volume: 132 Issue: 5 Pages: 629-640
  • DOI: 10.1007/s10265-019-01124-8
  • NAID: 40022011626
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] LSD1-LIKE1-Mediated H3K4me2 Demethylation Is Required for Homologous Recombination Repair (2019)
  • Author(s): Hirakawa Takeshi、Kuwata Keiko、Gallego Maria E.、White Charles I.、Nomoto Mika、Tada Yasuomi、Matsunaga Sachihiro
  • Journal Title: Plant Physiology Volume: 181 Issue: 2 Pages: 499-509
  • DOI: 10.1104/pp.19.00530
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Primed histone demethylation regulates shoot regenerative competency. (2019)
  • Author(s): Ishihara H, Sugimoto K, Tarr PT, Temman H, Kadokura S, Inui Y, Sakamoto T, Sasaki T, Aida M, Suzuki T, Inagaki S, Morohashi K, Seki M, Kakutani T, Meyerowitz EM, Matsunaga S.
  • Journal Title: Nat Commun Volume: 16 Issue: 1 Pages: 1786-1786
  • DOI: 10.1038/s41467-019-09386-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cyanidioschyzon merolae aurora kinase phosphorylates evolutionarily conserved sites on its target to regulate mitochondrial division (2019)
  • Author(s): Kato Shoichi、Okamura Erika、Matsunaga Tomoko M.、Nakayama Minami、Kawanishi Yuki、Ichinose Takako、Iwane Atsuko H.、Sakamoto Takuya、Imoto Yuuta、Ohnuma Mio、Nomura Yuko、Nakagami Hirofumi、Kuroiwa Haruko、Kuroiwa Tsuneyoshi、Matsunaga Sachihiro
  • Journal Title: Communications Biology Volume: 2 Issue: 1 Pages: 477-477
  • DOI: 10.1038/s42003-019-0714-x
  • Peer Reviewed / Open Access
• [Journal Article] Pyrenocine A induces monopolar spindle formation and suppresses proliferation of cancer cells (2019)
  • Author(s): Myobatake Yusuke、Kamisuki Shinji、Tsukuda Senko、Higashi Tsunehito、Chinen Takumi、Takemoto Kenji、Hachisuka Masami、Suzuki Yuka、Takei Maya、Tsurukawa Yukine、Maekawa Hiroaki、Takeuchi Toshifumi、Matsunaga Tomoko M.、Sahara Hiroeki、Usui Takeo、Matsunaga Sachihiro、Sugawara Fumio
  • Journal Title: Bioorganic & Medicinal Chemistry Volume: 27 Issue: 23 Pages: 115149-115149
  • DOI: 10.1016/j.bmc.2019.115149
  • Peer Reviewed / Open Access
• [Presentation] シロイヌナズナの核膜孔複合体によるセントロメア配置制御機構の解析 (2022)
  • Author(s): 伊藤ななみ、坂本卓也、坂本勇貴、松永幸大
  • Organizer: 第63回日本植物生理学会年会
• [Presentation] カルスを介したシュート再生系にLDL2が与える影響の解析 (2022)
  • Author(s): 堀江綾香、坂本卓也、Diaz Mariana、鈴木孝柾、松永幸大
  • Organizer: 第63回日本植物生理学会年会
• [Presentation] ガンマ線照射によるシュート再生能力向上のメカニズム解析 (2021)
  • Author(s): 東海林朋佳, 坂本勇貴, 鈴木孝征, 乾弥生, 坂本卓也, 松永幸大
  • Organizer: 日本植物形態学会第33回大会
• [Presentation] シロイヌナズナのカルスを介したシュート再生系にLDL2が与える影響の解析 (2021)
  • Author(s): 堀江綾香, 坂本卓也, Diaz Mariana, 鈴木孝柾, 松永幸大
  • Organizer: 日本植物形態学会第33回大会
• [Presentation] 核膜孔複合体によるセントロメア配置制御機構の解析 (2021)
  • Author(s): 伊藤ななみ, 坂本卓也, 坂本勇貴, 松永幸大
  • Organizer: 日本植物形態学会第33回大会
• [Presentation] 植物の核膜内膜に存在する新奇タンパク質の同定 (2021)
  • Author(s): 秋山義樹, 澁田未央, 坂本勇貴, 乾弥生, 松永朋子, 坂本卓也, 松永幸大
  • Organizer: 日本植物形態学会第33回大会
• [Presentation] 植物の根のDNA損傷における光受容体の機能の解析 (2021)
  • Author(s): 福田智代, 坂本卓也, 藤原唯, 乾弥生, 鈴木孝征, 松永幸大
  • Organizer: 日本植物形態学会第33回大会
• [Presentation] カルスの再生能獲得に関与するエピジェネティック制御因子の機能解析 (2021)
  • Author(s): 三浦 理奈, 坂本 卓也, 澁田 未央, 大矢 恵代, 稲垣 宗一, 鈴木 穣, 角谷 徹仁, 松永 幸大
  • Organizer: 日本植物形態学会第33回大会
• [Presentation] シロイヌナズナの新規器官再生系に関与する青色光受容体CRY1の機能解析 (2021)
  • Author(s): 山本一成, 坂本卓也, 乾弥生, 鈴木穣, 花田耕介, 松下智直, 松永幸大
  • Organizer: 日本植物形態学会第33回大会
• [Presentation] シュート再生における光受容体CRY1の機能解析 (2021)
  • Author(s): 山本一成, 坂本卓也, 乾弥生, 鈴木穣, 花田耕介, 松下智直, 松永幸大
  • Organizer: 日本植物学会第85回大会
• [Presentation] 植物の根のDNA損傷における光受容体の機能の解析 (2021)
  • Author(s): 福田智代, 坂本卓也, 藤原唯, 乾弥生, 鈴木孝征, 松永幸大
  • Organizer: 日本植物学会第85回大会
• [Presentation] シロイヌナズナにおいて核膜孔複合体はセントロメア配置制御をする (2021)
  • Author(s): 伊藤ななみ, 坂本卓也, 坂本勇貴, 松永幸大
  • Organizer: 日本植物学会第85回大会
• [Presentation] γ線照射によるシュート再生能力向上のメカニズム解析 (2021)
  • Author(s): 東海林朋佳, 坂本勇貴, 鈴木孝征, 坂本卓也, 松永幸大
  • Organizer: 日本植物学会第85回大会
• [Presentation] シロイヌナズナにおける、LDL2がカルスを介した再生に与える影響の解析 (2021)
  • Author(s): 堀江綾香, 坂本卓也, Diaz Mariana, 鈴木孝柾, 松永幸大
  • Organizer: 日本植物学会第85回大会
• [Presentation] カルスの再生能の獲得に関与するエピジェネティック制御因子の機能解析 (2021)
  • Author(s): 三浦 理奈, 坂本 卓也, 澁田 未央, 大矢 恵代, 稲垣 宗一, 鈴木 穣, 角谷 徹仁, 松永 幸大
  • Organizer: 日本植物学会第85回大会
• [Presentation] 植物での新奇核膜内膜タンパク質の同定 (2021)
  • Author(s): 秋山義樹, 澁田未央, 坂本勇貴, 乾弥生, 松永朋子, 坂本卓也, 松永幸大
  • Organizer: 日本植物学会第85回大会
• [Presentation] 植物の再生能の獲得に関与するエピジェネティック制御因子の機能解析 (2021)
  • Author(s): 三浦 理奈, 坂本 卓也, 澁田 未央, 大矢 恵代, 稲垣 宗一, 鈴木 穣, 角谷 徹仁, 松永 幸大
  • Organizer: 生物環境イノベーション研究部門第2回シンポジウム
• [Presentation] ガンマ線照射によるシュート再生能向上のメカニズム解析 (2021)
  • Author(s): 東海林朋佳, 坂本勇貴, 鈴木孝征, 乾弥生, 坂本卓也, 松永幸大
  • Organizer: 生物環境イノベーション研究部門第2回シンポジウム
• [Presentation] 植物の核膜孔複合体によるセントロメア配置制御機構の解析 (2021)
  • Author(s): 伊藤ななみ, 坂本卓也, 坂本勇貴, 松永幸大
  • Organizer: 第44回日本分子生物学会年会
• [Presentation] カルスの再生能力の獲得に関与するエピジェネティック制御因子の機能解析 (2021)
  • Author(s): 三浦 理奈, 坂本 卓也, 澁田 未央, 大矢 恵代, 稲垣 宗一, 鈴木 穣, 角谷 徹仁, 松永 幸大
  • Organizer: 第44回日本分子生物学会年会
• [Presentation] ガンマ線照射がもたらすシュート再生能力向上のメカニズム解析 (2021)
  • Author(s): 東海林朋佳, 坂本勇貴, 鈴木孝征, 坂本卓也, 松永幸大
  • Organizer: 第44回日本分子生物学会年会
• [Presentation] Analysis of centromere distribution mechanism by plant nuclear pore complex (2021)
  • Author(s): Nanami Ito, Takuya Sakamoto, Yuki Sakamoto, Sachihiro Matsunaga
  • Organizer: Plant genome stability and change 2020
  • Int'l Joint Research
• [Presentation] シロイヌナズナの核膜孔複合体はセントロメア配置制御を行う (2021)
  • Author(s): 伊藤 ななみ、坂本 卓也、坂本 勇貴、松永 幸大
  • Organizer: 第62回日本植物生理学会
• [Presentation] γ線照射によるシュート再生能向上機構の解析 (2021)
  • Author(s): 東海林朋佳、坂本勇貴、鈴木孝征、坂本卓也、松永幸大
  • Organizer: 第62回日本植物生理学会
• [Presentation] 植物の再生能力の獲得に関与するエピジェネティック制御因子の機能解析 (2021)
  • Author(s): 三浦理奈、坂本卓也、澁田未央、大矢恵代、稲垣宗一、鈴木穣、角谷徹二、松永幸大
  • Organizer: 第62回日本植物生理学会
• [Presentation] 植物の核膜孔複合体はセントロメア配置制御を行う (2020)
  • Author(s): 伊藤 ななみ、坂本 卓也、坂本 勇貴、松永 幸大
  • Organizer: 第43回日本分子生物学会
• [Presentation] Nuclear lamina protein CRWN regulates gene positioning of a copper transport family gene locus (2019)
  • Author(s): Yuki Sakamoto、Mayuko Sato、Takamasa Suzuki、Kiminori Toyooka、Shingo Takagi、Sachihiro Matsunaga
  • Organizer: 日本植物生理学会
• [Presentation] Elucidation of chromosome structure and epigenetic mechanism of gene regulation in primitive red alga Cyanidioschyzon merolae (2019)
  • Author(s): Minami Nakayama、Takuya Sakamoto、Tomoko Matsunaga、Miyako Kitagawa、Kan Tanaka、Tokiaki Takemura、Takamasa Suzuki、Sachihiro Matsunaga
  • Organizer: 日本植物生理学会
• [Presentation] Histone demethylase LDL1 promotes homologous recombination repair via demethylation of H3K4me2 (2019)
  • Author(s): Takeshi Hirakawa、Keiko Kuwata、Sachihiro Matsunaga
  • Organizer: 日本植物生理学会
• [Presentation] Analysis of relationship between maintenance of heat stress memory and chromosome higher order structure (2019)
  • Author(s): Yui Fujiwara、Takuya Sakamoto、Yuki Sakamoto、Nobutoshi Yamaguchi、Toshiro Ito、Sachihiro Matsunaga
  • Organizer: 日本植物生理学会
• [Presentation] Chromatin remodeling factors are required for de-novo shoot regeneration in Arabidopsis thaliana (2019)
  • Author(s): Mariana Diaz、Kaoru Sugimoto、Takuya Sakamoto、Sachihiro Matsunaga
  • Organizer: 日本植物生理学会
• [Presentation] Histone demethylation is involved in gene priming for plant regeneration (2019)
  • Author(s): Sachihiro Matsunaga、Hiroya Ishihara、Haruka Tenman、Satoshi Kadokura、Soichi Inagaki、Yayoi Inui、Takuya Sakamoto、Takamasa Suzuki、Kengo Morohashi、Tetsuji Kakutani、Kaoru Sugimoto
  • Organizer: 日本植物生理学会
• [Presentation] ASHH2 Regulates Arabidopsis callus formation and shoot regeneration through photosynthesis/light and glucose metabolism pathways (2019)
  • Author(s): Ryosuke Makino、Kaoru Sugimoto、Yuki Katsuyama、Hiroya Ishihara、Satoshi Kadokura、Takamasa Suzuki、Takuya Sakamoto、Sachihiro Matsunaga
  • Organizer: 日本植物生理学会
• [Presentation] Visualization of histone modification using live-imaging tool in plant (2019)
  • Author(s): Megumi Matsuoka、Takuya Sakamoto、Mio Shibuta、Noriyoshi Yagi、Hiroshi Kimura、Sachihiro Matsunaga
  • Organizer: 日本植物生理学会
• [Presentation] Transcriptome analysis reveals dynamic expression changes during tuberous root formation in Arabidopsis thaliana (2019)
  • Author(s): Sakiko Nishioka、Takuya Sakamoto、Takamasa Suzuki、Sachihiro Matsunaga
  • Organizer: 日本植物生理学会
• [Presentation] Analysis of relationship between maintenance of heat stress memory and chromosome higher order structure (2019)
  • Author(s): Sayuri Ando, Takumi Ogawa, Shoko Kojima, Yuki Sakamoto, Sachihiro Matsunaga, Yasunori Machida, and Chiyoko Machida
  • Organizer: Taiwan-Japan 2019 Plant Biology Conference
  • Int'l Joint Research
• [Presentation] シロイヌナズナの新規染色体構造構築制御因子の探索 (2019)
  • Author(s): 坂本卓也, 坂本勇貴, 御子侑香, 伊藤ななみ, 松永幸大
  • Organizer: 日本植物形態学会
• [Presentation] シロイヌナズナにおける条件特異的な塊根様組織形成メカニズムの解析 (2019)
  • Author(s): 西岡咲子, 坂本卓也, 鈴木孝征, 安江啓人, 諸橋賢吾, 松永幸大
  • Organizer: 日本植物形態学会
• [Presentation] 植物核ラミナ CRWN は核内の遺伝子配置に関与する (2019)
  • Author(s): 坂本勇貴, 高木慎吾, 松永幸大
  • Organizer: 日本植物形態学会
• [Presentation] Primed histone demethylation regulates shoot regenerative competency. (2019)
  • Author(s): 松永 幸大
  • Organizer: 日本植物形態学会
• [Presentation] 茎頂組織における体細胞胚形成の解析 (2019)
  • Author(s): 伊藤ななみ, 角倉慧, 杉本薫, 坂本卓也, 鈴木 孝征, 松永幸大
  • Organizer: 日本植物形態学会
• [Presentation] 植物の根の DNA 損傷応答に与える光の影響の解析 (2019)
  • Author(s): 藤原維, 坂本卓也, 松永幸大
  • Organizer: 日本植物形態学会
• [Presentation] 遺伝子プライミングによる再生メカニズム (2019)
  • Author(s): 松永 幸大，松岡 慈，澁田 未央，石原 弘也，天満 春花，角倉 慧，稲垣宗一，乾 弥生，鈴木 孝征，諸橋 賢吾，角谷 徹仁，杉本 薫，坂本 卓也
  • Organizer: 日本植物学会
• [Presentation] シロイヌナズナの新規染色体構造構築制御因子の解析 (2019)
  • Author(s): 坂本 卓也，坂本 勇貴，御子 侑香，伊藤 ななみ，松永 幸大
  • Organizer: 日本植物学会
• [Presentation] シロイヌナズナ茎頂組織における体細胞胚形成の解析 (2019)
  • Author(s): 伊藤 ななみ，角倉 慧，杉本 薫，鈴木 孝征，松永 幸大
  • Organizer: 日本植物学会
• [Presentation] シロイヌナズナ 19S プロテアソーム変異体における塊根様組織形成を制御する因子の探索 (2019)
  • Author(s): 西岡 咲子，坂本 卓也，鈴木 孝征，諸橋 賢吾，安江 啓人，松永 幸大
  • Organizer: 日本植物学会
• [Presentation] DNA 損傷応答に与える光の影響の解析 (2019)
  • Author(s): 藤原 維，坂本 卓也，松永 幸大
  • Organizer: 日本植物学会
• [Presentation] 核ラミナタンパク質 CRWN が環境応答遺伝子の発現を制御するメカニズムの解析 (2019)
  • Author(s): 坂本 勇貴，佐藤 繭子，豊岡 公徳，佐藤 良勝，高木 慎吾，松永 幸大
  • Organizer: 日本植物学会
• [Presentation] 植物における新奇核膜内膜局在タンパク質の同定 (2019)
  • Author(s): 渡邉水音、坂本勇貴、坂本卓也、松永幸大
  • Organizer: 日本分子生物学会
• [Presentation] シロイヌナズナの間期セントロメア配置制御に核膜孔複合体が関与する (2019)
  • Author(s): 御子侑香、坂本卓也、伊藤ななみ、坂本勇貴、松永幸大
  • Organizer: 日本分子生物学会
• [Presentation] Involvements of nuclear pore complex proteins in the regulation of spatial arrangement of chromatin domains in Arabidopsis (2019)
  • Author(s): Takuya Sakamoto、Yuka Oko、 Nanami Ito、Yuki Sakamoto、 Sachihiro Matsunaga
  • Organizer: SEB conference on Imapct of chromatin domains on plant phenotypes"
  • Int'l Joint Research
• [Presentation] FASCIATA1 is required for de-novo shoot regeneration in Arabidopsis thaliana (2019)
  • Author(s): Mariana Diaz、Takuya Sakamoto、 Sachihiro Matsunaga
  • Organizer: SEB conference on Imapct of chromatin domains on plant phenotypes"
  • Int'l Joint Research
• [Presentation] Live imaging system to tracking post-translational modification dynamics in Arabidopsis thaliana (2019)
  • Author(s): Mio K. Shibuta, Megumi Matsuoka, Mayu Yoshikawa, Kazuki Kurita, Tamako Yamaoka, Takuya Sakamoto, Hiroshi Kimura, Sachihiro Matsunaga
  • Organizer: IMPACT OF CHROMATIN DOMAINS ON PLANT PHENOTYPES
  • Int'l Joint Research
• [Remarks] 松永研究室
  • URL: http://park.itc.u-tokyo.ac.jp/matsunaga_lab/
• [Remarks] 環境記憶
  • URL: https://www.rs.tus.ac.jp/plantmemory/
• [Remarks] クロマチン・ダイナミクスから植物の生きざまを知る
  • URL: https://interview.plant-resilience.jp/sachihiro_matsunaga/