| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2022: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
Macrophages phagocytose hemophagocytes upon triple stimulation with CpG DNA, IFN-γ, and anti-IL-10 receptor antibody.Therefore, by comparing gene expression in macrophages before and after stimulation, we identified 12 cell surface molecules whose expression was strongly upregulated by the triple stimulation. By evaluating inhibitory antibodies against each molecule and the effect of decreased expression in macrophages using CRISPR/Cas9 on hemophagocytosis ability, four molecules were identified as novel hemophagocytosis-related molecules. We also analyzed how the expression of these molecules is regulated by signal crosstalk between CpG DNA and IFN-γ using the CAGE method and found that the transcription start sites of seven specific genes are altered.
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